Macromolecular Crowding Regulates Assembly of mRNA Stress Granules after Osmotic Stress

Bounedjah, Ouissame; Hamon, Loïc; Savarin, Philippe; Desforges, Bénédicte; Curmi, Patrick A.; Pastré, David

doi:10.1074/jbc.m111.292748

Cited by 94 publications

(79 citation statements)

References 76 publications

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“…Despite their inability to be affected by CHX or puromycin after they are formed, NaCl-induced foci are prevented by pretreatment with CHX (Kedersha et al, 2016), suggesting that they are more stable than canonical SGs. As the proposed mechanism of their formation is driven by molecular crowding due to osmotic stress (Bounedjah et al, 2012) and as their formation is independent of p-eIF2α (Bevilacqua et al, 2010;Kedersha et al, 2016), their stability may indicate an energetic barrier to SG disassembly.…”

Section: Discussionmentioning

confidence: 99%

Stress-specific differences in assembly and composition of stress granules and related foci

Aulas

Fay

Lyons

et al. 2017

Journal of Cell Science

252

301

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Cells have developed different mechanisms to respond to stress, including the formation of cytoplasmic foci known as stress granules (SGs). SGs are dynamic and formed as a result of stress-induced inhibition of translation. Despite enormous interest in SGs due to their contribution to the pathogenesis of several human diseases, many aspects of SG formation are poorly understood. SGs induced by different stresses are generally assumed to be uniform, although some studies suggest that different SG subtypes and SG-like cytoplasmic foci exist. Here, we investigated the molecular mechanisms of SG assembly and characterized their composition when induced by various stresses. Our data revealed stress-specific differences in composition, assembly and dynamics of SGs and SGlike cytoplasmic foci. Using a set of genetically modified haploid human cells, we determined the molecular circuitry of stress-specific translation inhibition upstream of SG formation and its relation to cell survival. Finally, our studies characterize cytoplasmic stress-induced foci related to, but distinct from, canonical SGs, and also introduce haploid cells as a valuable resource to study RNA granules and translation control mechanisms.

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Section: Discussionmentioning

confidence: 99%

Stress-specific differences in assembly and composition of stress granules and related foci

Aulas

Fay

Lyons

et al. 2017

Journal of Cell Science

252

301

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“…The results indicate that nature probably can only use osmolyte for protein stabilization as a broad stroke tool for the universal peptide backbone but individual proteins and tissues will also need other mechanisms for the complete counteraction (e. g., protein-genetic evolution, other situation specific osmolytes and presence of salts (Rahman et al, 2015) etc). In addition, osmolytic compounds can act as nano-crowders (Bounedjah et al, 2012;Cho et al, 2011;Ma et al, 2014) and thus may help to make different ratios appropriate in different cells. These hypotheses need more careful study to come to the conclusions.…”

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confidence: 99%

A current perspective on the compensatory effects of urea and methylamine on protein stability and function

Rahman

Warepam

Singh

et al. 2015

Progress in Biophysics and Molecular Biology

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“…In general, understanding the influence of mechanical stress on cancer growth could shed new light on tumour development and progression. Studies present in the literature also use hypertonic conditions, but the effects are always observed at high osmotic pressure [12,13].…”

Section: Introductionmentioning

confidence: 99%

Osmotic stress affects functional properties of human melanoma cell lines

et al. 2015

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Abstract. Understanding the role of microenvironment in cancer growth and metastasis is a key issue for cancer research. Here, we study the effect of osmotic pressure on the functional properties of primary and metastatic melanoma cell lines. In particular, we experimentally quantify individual cell motility and transmigration capability. We then perform a circular scratch assay to study how a cancer cell front invades an empty space. Our results show that primary melanoma cells are sensitive to a low osmotic pressure, while metastatic cells are less. To better understand the experimental results, we introduce and study a continuous model for the dynamics of a cell layer and a stochastic discrete model for cell proliferation and diffusion. The two models capture essential features of the experimental results and allow to make predictions for a wide range of experimentally measurable parameters. PACS. PACS-key discribing text of that key -PACS-key discribing text of that key

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Macromolecular Crowding Regulates Assembly of mRNA Stress Granules after Osmotic Stress

Cited by 94 publications

References 76 publications

Stress-specific differences in assembly and composition of stress granules and related foci

Stress-specific differences in assembly and composition of stress granules and related foci

A current perspective on the compensatory effects of urea and methylamine on protein stability and function

Osmotic stress affects functional properties of human melanoma cell lines

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