Macromolecular synthesis in cells infected by frog virus — VIII. The nucleus is a site of frog virus 3 DNA and RNA synthesis

Goorha, Rakesh; Murti, Gopal; Granoff, Allan; Tirey, Ramona

doi:10.1016/0042-6822(78)90216-7

Cited by 79 publications

(54 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They possess a linear double-stranded DNA (dsDNA) genome with circular permutation and terminal redundancy (6,13), and replication of the viral genome includes distinct nuclear and cytoplasmic phases (12). The genomes are encapsidated within an icosahedral shell ranging between 120 and 180 nm in diameter and comprised predominantly of a 50-kDa major capsid protein (MCP).…”

mentioning

confidence: 99%

Genomic and Proteomic Analysis of Invertebrate Iridovirus Type 9

Wong

Young

Kleffmann

et al. 2011

J Virol

View full text Add to dashboard Cite

Iridoviruses (IV) are nuclear cytoplasmic large DNA viruses that are receiving increasing attention as sublethal pathogens of a range of insects. Invertebrate iridovirus type 9 (IIV-9; Wiseana iridovirus) is a member of the major phylogenetic group of iridoviruses for which there is very limited genomic and proteomic information. The genome is 205,791 bp, has a G؉C content of 31%, and contains 191 predicted genes, with approximately 20% of its repeat sequences being located predominantly within coding regions. The repeated sequences include 11 proteins with helix-turn-helix motifs and genes encoding related tandem repeat amino acid sequences. Of the 191 proteins encoded by IIV-9, 108 are most closely related to orthologs in IIV-3 (Chloriridovirus genus), and 114 of the 126 IIV-3 genes have orthologs in IIV-9. In contrast, only 97 of 211 IIV-6 genes have orthologs in IIV-9. There is almost no conservation of gene order between IIV-3, IIV-6, and IIV-9. Phylogenetic analysis using a concatenated sequence of 26 core IV genes confirms that IIV-3 is more closely related to IIV-9 than to IIV-6, despite being from a different genus of the Iridoviridae. An interaction between IIV and small RNA regulatory systems is supported by the prediction of seven putative microRNA (miRNA) sequences combined with XRN exonuclease, RNase III, and double-stranded RNA binding activities encoded on the genome. Proteomic analysis of IIV-9 identified 64 proteins in the virus particle and, when combined with infected cell analysis, confirmed the expression of 94 viral proteins. This study provides the first full-genome and consequent proteomic analysis of group II IIV.

show abstract

mentioning

confidence: 99%

Genomic and Proteomic Analysis of Invertebrate Iridovirus Type 9

Wong

Young

Kleffmann

et al. 2011

J Virol

View full text Add to dashboard Cite

show abstract

“…Whilst it is clear that a large proportion of total virus DNA is synthesized in association with the cell nucleus, there is no evidence that this is eventually relocated in the cytoplasm a.s demonstrated with frog virus-3 (Goorha et aL, 1978). However, it is established that vaccinia virus-specific DNA synthesis is highly redundant and that the bulk is not incorporated into progeny virus particles but remains susceptible to experimental degradation by DNase L.…”

Section: Discussionmentioning

confidence: 90%

Synthesis of Full-length, Virus Genomic DNA by Nuclei of Vaccinia-infected HeLa Cells

Archard

1983

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYIt is well established that vaccinia virus infection induces the synthesis of virusspecific DNA in cytoplasmic 'factories', which are sites of virus-specific transcription. The present study demonstrates that vaccinia virus-specific DNA is synthesized also in the nuclei of infected cells with a similar time course. Direct observation and radiolabelling confirm the integrity of isolated nuclei. Reconstitution experiments and inhibitor studies demonstrate that virus-induced DNA is synthesized de novo within nuclei and does not result from cytoplasmic contamination. Cell-specific DNA synthesis is inhibited completely after infection and nuclei of infected cells then synthesize DNA which co-sediments with virus genomic DNA in denaturing gradients. Restriction endonuclease cleavage and hybridization with a virus-specific probe indicate that this is full-length, virus genomic DNA. The biological implications of this are discussed.

show abstract

“…Frog virus 3, also a member of the family Iridoviridae, has been reported to replicate in the cytoplasm and in the nucleus of the host cell and viral transcription depends on cellular RNA polymerase II (Goorha et al, 1978). No DdRP of this virus has been described so far.…”

Section: Discussionmentioning

confidence: 99%