Macromolecular Transport in Jejunal Mucosa of Children with Severe Malnutrition

Heyman, Martine; Boudraa, G.; Sarrut, S; Giraud, Maud; Evans, Lynleigh; Touhami, M.; Desjeux, Jehan-François

doi:10.1097/00005176-198406000-00009

Cited by 61 publications

(33 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The electrical parameters (Table 3) obtained in control biopsies were similar to those obtained previously in children recovering from malnutrition (8), and in healthy children previously studied (19). The 95% confidence interval for Isc was 2 1.5 to 39.05 PA. ~m -~.…”

Section: Resultssupporting

confidence: 79%

“…Control children absorbed HRP by the two functional pathways previously described (7,8) to a similar extent in the age range from 2 months to 11 yr. A large increase in HRP endocytosis, associated with an increase in short-circuit current, was found during the initial period of CMA when infants less than 3 months old underwent inadvertent or deliberate exposure to cow's milk antigens. These alterations were not associated with any significant change in electrical tissue conductance.…”

Section: Discussionmentioning

confidence: 61%

“…HRP (Sigma type VI, Sigma Chemical Co., St. Louis, MO) was used as described earlier (7,8) to quantitate permeability to the protein in its intact and degraded forms. Briefly, HRP at a final concentration of 10 pM (0.4 mg/ml) was added to the mucosal compartment and [3H]HRP, prepared and stored as previously reported (7), was also added as a tracer (1.33 pCi/ml).…”

Section: Methodsmentioning

confidence: 99%

“…40,000). We showed that this protein was transported across the intestinal epithelium by endocytosis along two functional pathways: a minor route allowing transfer of the intact protein and a major route involving lysosomal degradation during transport (7,8). In infants, serial intestinal biopsies have been recommended before and after open challenges to further confirm the diagnosis of CMA (9-1 1).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Antigen Absorption by the Jejunal Epithelium of Children with Cow's Milk Allergy

Grasset²,

et al. 1988

Self Cite

View full text Add to dashboard Cite

ABSTRACT. To establish if intestinal permeability to exogenous antigens is involved in cow's milk allergy (CMA) in infants, 33 children 1 to 24 months old (18 controls and 1 5 with CMA) were tested for intestinal permeability to the protein marker horseradish peroxidase (HRP). Jejunal biopsies were performed either during the initial period of diagnosis, at the mean (and SE) age of 3 f 1 months, and/or 1 yr later, at the age of 13 f 2 months, just before and after a milk challenge. A small fragment of the biopsy was studied for histology and the remainder was mounted in an Ussing chamber for simultaneous measurement of mucosal to serosal transport of H R P in its intact and degraded forms and electrical parameters including short-circuit current and conductance. No modification in H R P absorption was noted in control children aged from 2 months to 11 yr, indicating that gut closure probably occurred earlier in life. During the initial period of CMA, transepithelial H R P fluxes were significantly higher, about 8-fold, in children with CMA (intact H R P flux = 48.5 f 15.2, 95% confidence interval, 11.2 to 85.7 versus 5.9 + 1.2, 95% confidence interval 2.9 to 8.3, in control children) In addition, short-circuit current was increased but conductance was unchanged. After several months on a milkfree diet, H R P flux and short-circuit current returned to control values. Just after the milk challenge and independently of -the clinical issue, a slight rise in H R P permeability was observed but it was not significant and remained within control values. These results suggest that increased permeability to proteins is probably not the primary cause of CMA, and that the increase in protein endocytosis noted during the initial period of CMA seems rather to be a secondary effect of an abnormal immunological response leading to mucosal inflammation and impairment of the endocytic process. (Pediatr Res 24:197-202, 1988)

show abstract

Section: Resultssupporting

confidence: 79%

Section: Discussionmentioning

confidence: 61%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Antigen Absorption by the Jejunal Epithelium of Children with Cow's Milk Allergy

Grasset²,

et al. 1988

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the sheep fetus the adrenal produces cortisol in response to hypophyseal ACTH from about 120 days onwards (Wintour, 1984). Plasma cortisol concentrations reach their maximum levels prior to birth, and at birth are still much higher than those in the adult (see Nathanielsz, 1976 (Heyman et al, 1984 ;Guiraldes and Hamilton 19811, thyroxine (see Yeh and Moog, 1977), insulin (Malo and Menard, 1983 ;Arsenault and Menard, 1984), cortisol (Herbst and Sunshine, 1969 …”

Section: Introductionmentioning

confidence: 99%

Perinatal development of the small intestine of the sheep

Trahair¹,

Robinson²

1986

Reprod. Nutr. Dévelop.

View full text Add to dashboard Cite

Impaired gut barrier function in malnourished patients

Reynolds¹,

O’Farrelly²,

Feighery³

et al. 1996

Br. J. Surg.

View full text Add to dashboard Cite

The gastrointestinal tract is essential to host defence, acting as a barrier to absorption and translocation of gut antigens, including bacteria. In experimental models, protein malnutrition is permissive to gut barrier failure and endogenous infection. A clinical correlate has not been described. Intestinal morphology and barrier function to food protein antigens was studied in malnourished patients. Thirty-five individuals were evaluated, 20 malnourished patients and 15 well nourished hospital controls. Morphology was assessed from endoscopic biopsies of the second part of the duodenum, and serum immunoglobulin (Ig) G antibodies to gliadin and beta-lactoglobulin were measured. No antibody to food proteins was evident in the control group. In contrast, serum IgG antibodies to at least one antigen were present in 15 malnourished patients and to both antigens in ten (P < 0.0001 versus controls). Severely malnourished patients were more likely to have both antibodies present than those with mild or moderate malnutrition (P < 0.05). Antibody-positive malnourished patients had significantly better nutritional status than antibody-negative patients with malnutrition (P < 0.05). In no group of patients was there morphological evidence of abnormal mucosa or an immunological infiltrate. Gut barrier function is compromised in malnourished patients which suggests a mechanism that may facilitate gut-derived infection and sepsis.

show abstract

Macromolecular Transport in Jejunal Mucosa of Children with Severe Malnutrition

Cited by 61 publications

References 0 publications

Antigen Absorption by the Jejunal Epithelium of Children with Cow's Milk Allergy

Antigen Absorption by the Jejunal Epithelium of Children with Cow's Milk Allergy

Perinatal development of the small intestine of the sheep

Impaired gut barrier function in malnourished patients

Contact Info

Product

Resources

About