Macromolecule Adsorption to Hemodialysis Membranes Depends on Molecular Size

Leypoldt, John K.; Gilson, Janice F.; Blindauer, Kim M.; Cheung, Alfred K.

doi:10.1159/000170074

Cited by 9 publications

(4 citation statements)

References 5 publications

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“…A proportion of the peptide is likely to be absorbed into the surface or channels of the cellulose membrane because of its affinity for hydrophilic film. A similar phenomenon has already been reported [80][81][82][83].…”

Section: Encapsulation Efficiency Of Ghk-cu In Liposomessupporting

confidence: 87%

Liposomes as Carriers of GHK-Cu Tripeptide for Cosmetic Application

Dymek,

Olechowska,

Hąc-Wydro

et al. 2023

Pharmaceutics

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Liposomes are self-assembled spherical systems composed of amphiphilic phospholipids. They can be used as carriers of both hydrophobic and hydrophilic substances, such as the anti-aging and wound-healing copper-binding peptide, GHK-Cu (glycyl-L-histidyl-L-lysine). Anionic (AL) and cationic (CL) hydrogenated lecithin-based liposomes were obtained as GHK-Cu skin delivery systems using the thin-film hydration method combined with freeze–thaw cycles and the extrusion process. The influence of total lipid content, lipid composition and GHK-Cu concentration on the physicochemical properties of liposomes was studied. The lipid bilayer fluidity and the peptide encapsulation efficiency (EE) were also determined. Moreover, in vitro assays of tyrosinase and elastase inhibition were performed. Stable GHK-Cu-loaded liposome systems of small sizes (approx. 100 nm) were obtained. The bilayer fluidity was higher in the case of cationic liposomes. As the best carriers, 25 mg/cm3 CL and AL hydrated with 0.5 mg/cm3 GHK-Cu were selected with EE of 31.7 ± 0.9% and 20.0 ± 2.8%, respectively. The obtained results confirmed that the liposomes can be used as carriers for biomimetic peptides such as copper-binding peptide and that the GHK-Cu did not significantly affect the tyrosinase activity but led to 48.90 ± 2.50% elastase inhibition, thus reducing the rate of elastin degeneration and supporting the structural integrity of the skin.

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Section: Encapsulation Efficiency Of Ghk-cu In Liposomessupporting

confidence: 87%

Liposomes as Carriers of GHK-Cu Tripeptide for Cosmetic Application

Dymek,

Olechowska,

Hąc-Wydro

et al. 2023

Pharmaceutics

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“…The adsorption capacity of AN69 membranes seems larger than that of other synthetic membranes, presumably because of its negatively charged surface and because of its symmetrical structure (32)(33)(34). The AN69 membrane has been shown to efficiently adsorb anaphylatoxins (C3a, C5a), bradykinin, endotoxins, and cytokines (IL-1, TNF), all factors that play a role in the pathophysiology of sepsis (35)(36)(37)(38).…”

Section: Discussionmentioning

confidence: 99%

Comparison of polyacrylonitrile (AN69) and polysulphone membrane during hemofiltration in canine endotoxic shock

Rogiers

Zhang

Pauwels

et al. 2003

Critical Care Medicine

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In this acute endotoxic shock model, CVVH with the polyacrylonitrile membrane improved cardiac performance when compared with the polysulphone membrane. These effects could be caused by a more effective adsorption of inflammatory mediators other than tumor necrosis factor. Whether the polyacrylonitrile membrane should be preferred over the polysulphone membrane for CVVH in severe sepsis warrants further experimental and clinical study.

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“…Dextrans are useful markers for characterizing the transport of large solutes during haemodialysis because they are inert and do not significantly interact or bind readily to haemodialysis membranes [28]. While dextrans are useful in describing general transport principles, their rates of removal may not be relevant for a particular uraemic toxin that is significantly bound either to plasma proteins or to haemodialysis membranes.…”

Section: Discussionmentioning

confidence: 99%

Removal of high-molecular-weight solutes during high-efficiency and high-flux haemodialysis

1996

Nephrology Dialysis Transplantation

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Background. Although urea clearance is often increased during high-efficiency and high-flux haemodialysis to compensate for short treatment times, the impact of these treatment modalities on the removal of larger uraemic toxins has not been thoroughly investigated. Methods. We compared solute removal rates for five haemodialysis treatment strategies in vitro using neutral dextrans (molecular radii between 15 and 50 A) as marker macromolecules. Removal rates were assessed by the decrease in dextran concentration within the reservoir of a model circuit using outdated human plasma as the test solution. Results for high-efficiency haemodialysis (CA110 dialyser at a blood flow rate of 400ml/min and TAF175 dialyser at a blood flow rate of 300ml/min) and high-flux haemodialysis (CT190G dialyser at a blood flow rate of 300 ml/min and F60 dialyser at a blood flow rate of 300 ml/min) were compared with those for conventional haemodialysis (CA110 dialyser at a blood flow rate of 200 ml/min). Results. Dextran clearances were dependent on the dialyser employed, and they decreased with molecular size and time for each treatment strategy. Removal rates were greatest using the CT190G and F60 dialysers, intermediate for the TAF175 dialyser, and lowest for the CA110 dialyser at either blood flow rate. Conclusions. The results of this study demonstrate that increasing blood flow rates alone to increase urea clearance may not provide adequate removal of highmolecular-weight solutes. The use of high-flux or large surface area, high-efficiency dialysers are more effective in maintaining the removal of high-molecular-weight solutes when treatment time is shortened.

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Macromolecule Adsorption to Hemodialysis Membranes Depends on Molecular Size

Cited by 9 publications

References 5 publications

Liposomes as Carriers of GHK-Cu Tripeptide for Cosmetic Application

Liposomes as Carriers of GHK-Cu Tripeptide for Cosmetic Application

Comparison of polyacrylonitrile (AN69) and polysulphone membrane during hemofiltration in canine endotoxic shock

Removal of high-molecular-weight solutes during high-efficiency and high-flux haemodialysis

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