MACROMOLECULE SYNTHESIS AND GERMINATION OF CONIDIA IN TEMPERATURE SENSITIVE MUTANTS OF &lt;i&gt;NEUROSPORA CRASSA&lt;/i&gt;

Inoue, Hirokazu; Ishikawa, Tatsuo

doi:10.1266/jjg.45.357

Cited by 31 publications

(21 citation statements)

References 25 publications

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“…The un-17 strain (FGSC2356) described as TS25 (T51M171) in the previous paper (Inoue and Ishikawa, 1970) was used. For his-3 targeted transformation of N. crassa, a strain his-3A (FGSC6103) was used as a host.…”

Section: Strains and Plasmidsmentioning

confidence: 99%

“…Effects of forced expression of the PAP-encoding genes on the phenotypes of wild-type strain or un-17 mutant The un-17 mutant shows a pleiotropic phenotype: heat sensitivity (Inoue and Ishikawa, 1970), female sterility (Perkins, unpublished), altered phospholipid synthesis (Goodrich-Tanrikulu, personal communication), cold sensitivity (Munkres, 1979), and deficiency of exocellular SOD, SOD4, activity (Munkres, 1992). We examined whether the forced expression of putative PAPencoding genes affected phenotypes of either the wildtype strain or the un-17mutant.…”

Section: Forced Expression Of the Flag-tagged Pap-encoding Genesmentioning

confidence: 99%

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Genetic and molecular analysis of the temperature-sensitive mutant un-17 carrying a mutation in the gene encoding poly(A)-polymerase in Neurospora crassa

et al. 2007

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The un-17 mutant was originally isolated as an irreparable temperature-sensitive (ts) mutant in Neurospora crassa. Early experiments showed that cells of this mutant immediately stopped growing and died when the temperature of the culture was shifted from a permissive temperature (25°C) to non-permissive temperature (35°C). This ts phenotype is suppressed by addition of cycloheximide or in some conditions of growth repression. Even at the permissive temperature, it shows a female sterile phenotype and is deficient in production of exocellular superoxide dismutase SOD4 (EC 1.15.1.1). By searching for a DNA fragment that complements the ts phenotype of the un-17 mutant from a N. crassa genome library, we found the un-17 gene. The cloned un-17 gene encodes a homolog of the Saccharomyces cerevisiae poly(A) polymerase (PAP). The un-17 mutant had a one-base substitution mutation in the gene. The cloned un-17 genes from the wild-type strain and the un-17 mutant were introduced into both the un-17 mutant and wild-type strain. The un-17 mutant introduced by un-17 DNA from the wild-type strain showed recovery of both the ts and female sterile phenotypes. Moreover, the purified product derived from the wild-type strain showed PAP activity in vitro. These findings indicate that the un-17 mutant carries a ts mutation in the gene encoding PAP.

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Section: Strains and Plasmidsmentioning

confidence: 99%

Section: Forced Expression Of the Flag-tagged Pap-encoding Genesmentioning

confidence: 99%

Genetic and molecular analysis of the temperature-sensitive mutant un-17 carrying a mutation in the gene encoding poly(A)-polymerase in Neurospora crassa

et al. 2007

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“…Conidia of wild-type strain 74A of Neurospora crassa were prepared as described previously.2) About 2 x 105 conidia were inoculated onto dialysis tubing (6.5 cm i.d., Union Carbide Co.) in a 100 ml conical flask containing 15 ml of glycerol complete agar medium (GCM), 4) followed by incubation at 26°C in the dark. At intervals, conidia and mycelia were harvested by means of suspension in distilled water, and the suspensions were filtered through two layers of cheese cloth to remove the mycelia, and the filtrates were centrifuged at 3000 rpm for 5 min to obtain the conidia.…”

Section: Responsiveness Of Neurospora Crassamentioning

confidence: 99%

Responsiveness ofNeurospora crassaConidia to Auxin and Gibberellin in Relation to Culture Age

Kawanabe

1986

Agricultural and Biological Chemistry

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“…crassa was used. Conidia, produced ona glycerol complete agar medium 9 ) .after 7 days of incubation of mycelia, were collected by suspending in distilled water, and washed twice by distilled water. These were referred to as conidia at the initial stage.…”

Section: Conid Ial Germinat Ionmentioning

confidence: 99%

Identification of GA₃inNeurospora crassaand Its Changes during Conidial Germination and Mycelial Growth

Kawanabe

Yamane²,

Takahashi³

et al. 1985

Agricultural and Biological Chemistry

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MACROMOLECULE SYNTHESIS AND GERMINATION OF CONIDIA IN TEMPERATURE SENSITIVE MUTANTS OF <i>NEUROSPORA CRASSA</i>

Cited by 31 publications

References 25 publications

Genetic and molecular analysis of the temperature-sensitive mutant un-17 carrying a mutation in the gene encoding poly(A)-polymerase in Neurospora crassa

Genetic and molecular analysis of the temperature-sensitive mutant un-17 carrying a mutation in the gene encoding poly(A)-polymerase in Neurospora crassa

Responsiveness ofNeurospora crassaConidia to Auxin and Gibberellin in Relation to Culture Age

Identification of GA₃inNeurospora crassaand Its Changes during Conidial Germination and Mycelial Growth

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MACROMOLECULE SYNTHESIS AND GERMINATION OF CONIDIA IN TEMPERATURE SENSITIVE MUTANTS OF <i>NEUROSPORA CRASSA</i>

Cited by 31 publications

References 25 publications

Genetic and molecular analysis of the temperature-sensitive mutant un-17 carrying a mutation in the gene encoding poly(A)-polymerase in Neurospora crassa

Genetic and molecular analysis of the temperature-sensitive mutant un-17 carrying a mutation in the gene encoding poly(A)-polymerase in Neurospora crassa

Responsiveness ofNeurospora crassaConidia to Auxin and Gibberellin in Relation to Culture Age

Identification of GA3inNeurospora crassaand Its Changes during Conidial Germination and Mycelial Growth

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Identification of GA₃inNeurospora crassaand Its Changes during Conidial Germination and Mycelial Growth