Macromolecules, X-Ray Diffraction of Biological

Messerschmidt, Albrecht; Huber, Robert

doi:10.1002/3527600906.mcb.200400058

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2012

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“…Assuming a protein density of 1.35 g/cm 3 (41) and taking the measured protein/phospholipid/CHS molar ratio into account, the density of the lipid matrix of proteoliposomes is estimated to be ϳ1.04 g/cm 3 , which is in reasonable agreement with expectations from density measurements on protein-free liposomes (42)(43)(44).…”

Section: Resultssupporting

confidence: 80%

Recombinant Cannabinoid Type 2 Receptor in Liposome Model Activates G Protein in Response to Anionic Lipid Constituents

Kimura

Yeliseev

Vukoti

et al. 2012

Journal of Biological Chemistry

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Background:Structural and functional studies on G protein-coupled receptor (GPCR) benefit from reconstitution of pure GPCR into lipid bilayers. Results: Cannabinoid CB 2 receptor was successfully reconstituted and the effect of anionic lipids and cholesterol on G protein activation was studied. Conclusion: G protein activation increased with anionic lipid content up to ϳ50 mol %. Significance: Anionic lipids regulate signal transduction by CB 2 receptor and possibly other class A GPCR.

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