Macrophage apoptosis using alendronate in targeted nanoarchaeosomes

Jerez, Horacio Emanuel; Altube, María Julia; Gándola, Yamila B.; González, Lorena; González, Marina; Morilla, María José; Romero, Eder Lilia

doi:10.1016/j.ejpb.2021.01.001

Cited by 12 publications

(12 citation statements)

References 71 publications

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“…The endocytosis of archaeolipids induces effects different from those of ordinary lipids composing nanoliposomes. On THP-1 macrophages, for instance, the endocytosis of archaeolipids is suggested to induce autophagy [ 73 ]. Physiological levels of autophagy are key for a correct wound healing and epithelial repair of the alveoli [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

Reparation of an Inflamed Air-Liquid Interface Cultured A549 Cells with Nebulized Nanocurcumin

et al. 2021

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The anti-inflammatory, antifibrotic and antimicrobial activities of curcumin (CUR) are missed because of its low solubility in aqueous media, low bioavailability, and structural lability upon oral intake. Soft nanoparticles such as nanoliposomes are not efficient as CUR carriers, since crystalline CUR is expelled from them to physiological media. Nanostructures to efficiently trap and increase the aqueous solubility of CUR are needed to improve both oral or nebulized delivery of CUR. Here we showed that SRA1 targeted nanoarchaeosomes (nATC) [1:0.4 w:w:0.04] archaeolipids, tween 80 and CUR, 155 ± 16 nm sized of −20.7 ± 3.3 z potential, retained 0.22 mg CUR ± 0.09 per 12.9 mg lipids ± 4.0 (~600 μM CUR) in front to dilution, storage, and nebulization. Raman and fluorescence spectra and SAXS patterns were compatible with a mixture of enol and keto CUR tautomers trapped within the depths of nATC bilayer. Between 20 and 5 µg CUR/mL, nATC was endocytosed by THP1 and A549 liquid–liquid monolayers without noticeable cytotoxicity. Five micrograms of CUR/mL nATC nebulized on an inflamed air–liquid interface of A549 cells increased TEER, normalized the permeation of LY, and decreased il6, tnfα, and il8 levels. Overall, these results suggest the modified pharmacodynamics of CUR in nATC is useful for epithelia repair upon inflammatory damage, deserving further deeper exploration, particularly related to its targeting ability.

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Section: Discussionmentioning

confidence: 99%

Reparation of an Inflamed Air-Liquid Interface Cultured A549 Cells with Nebulized Nanocurcumin

et al. 2021

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“…Hence, the number ratio of wear particles to cells was 10:1 or so. Additionally, previous studies showed that bisphosphonates, such as ALN, could inhibit the proliferation and induce the death of macrophages when the concentration was within a certain range (10 −9 –10 −4 M) [ 23 , 24 ]. It was calculated that the cumulative release of ALN in the first 14 d was within the above effective concentration range (up to 1.2 × 10 −5 M) according to the profile of ALN release in our previous study [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Insight into the Molecule Impact of Critical-Sized UHMWPE-ALN Wear Particles on Cells by the Alginate-Encapsulated Cell Reactor

Liu

Shi

2023

IJMS

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Wear particles of ultra-high molecular weight polyethylene (UHMWPE) are inevitable during service as joint prosthesis, and particles ≤ 10 μm with critical size could cause serious osteolysis and aseptic loosening of joint prosthesis. The aim of this study is to adopt the alginate-encapsulated cell reactor to investigate the molecular impact of critical-sized wear particles of UHMWPE loaded with alendronate sodium (UHMWPE-ALN) on cells. Results showed that compared with UHMWPE wear particles, UHMWPE-ALN wear particles inhibited the proliferation of macrophages significantly after being co-cultured for 1, 4, 7, and 14 d. Furthermore, the released ALN promoted early apoptosis, suppressed the secretion of TNF-α and IL-6 of macrophages, and down-regulated relative gene expressions of TNF-α, IL-6, and IL-1β and RANK. In addition, compared with UHMWPE wear particles, UHMWPE-ALN wear particles promoted the ALP activity of osteoblasts, down-regulated the gene expression of RANKL, and up-regulated gene expression of osteoprotegerin. There were mainly two approaches of the effects of critical-sized UHMWPE-ALN wear particles on cells, one of which was cytology and the other was cytokine signal pathway. The former mainly affected the proliferation and activity of macrophages and osteoblasts. The latter would inhibit osteoclasts via cytokine and RANKL/RANK signal pathway. Thus, UHMWPE-ALN had the potential application in clinics to treat osteolysis induced by wear particles.

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“…Moreover, only a handful of studies have been performed to elucidate the role of NA as a stable drug carrier for cancer therapeutics. [15][16][17] In this aspect, in our study, we have synthesized and characterized the NA as a thermostable drug carrier system for cancer therapeutics, investigated their sustained drug release potential, and unravelled the cytotoxic mechanism in MCF-7 breast cancer cells. The NA was prepared by using archaeal lipid derived from the hyperthermophilic archaeon Aeropyrum pernix K1.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, only a handful of studies have been performed to elucidate the role of NA as a stable drug carrier for cancer therapeutics. 15–17…”

Section: Introductionmentioning

confidence: 99%

Doxorubicin loaded thermostable nanoarchaeosomes: a next-generation drug carrier for breast cancer therapeutics

Babunagappan,

Seetharaman,

Ariraman

et al. 2024

Nanoscale Adv.

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Macrophage apoptosis using alendronate in targeted nanoarchaeosomes

Cited by 12 publications

References 71 publications

Reparation of an Inflamed Air-Liquid Interface Cultured A549 Cells with Nebulized Nanocurcumin

Reparation of an Inflamed Air-Liquid Interface Cultured A549 Cells with Nebulized Nanocurcumin

Insight into the Molecule Impact of Critical-Sized UHMWPE-ALN Wear Particles on Cells by the Alginate-Encapsulated Cell Reactor

Doxorubicin loaded thermostable nanoarchaeosomes: a next-generation drug carrier for breast cancer therapeutics

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