Macrophage-derived small extracellular vesicles promote biomimetic mineralized collagen-mediated endogenous bone regeneration

Liu, Anqi; Jin, Shanshan; Fu, Cuicui; Cui, Shengji; Zhang, Ting; Zhu, Lisha; Wang, Yu; Shen, Steve Guofang; Jiang, Nan; Liu, Yan

doi:10.1038/s41368-020-00100-6

Cited by 44 publications

(37 citation statements)

References 41 publications

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“… 14 The regulatory effect of macrophages on bone stromal cells has been confirmed. 15 , 16 Mice depleted of macrophages showed an osteoporotic phenotype. 17 In aged mice that received bone marrow macrophages transplantation from young mice, transplanted macrophage infiltration was observed at the fracture site and the healing of fracture was accelerated.…”

Section: Introductionmentioning

confidence: 99%

Mapping the immune microenvironment for mandibular alveolar bone homeostasis at single-cell resolution

Lin

Zhang

et al. 2021

Bone Res

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Alveolar bone is the thickened ridge of jaw bone that supports teeth. It is subject to constant occlusal force and pathogens invasion, and is therefore under active bone remodeling and immunomodulation. Alveolar bone holds a distinct niche from long bone considering their different developmental origin and postnatal remodeling pattern. However, a systematic explanation of alveolar bone at single-cell level is still lacking. Here, we construct a single-cell atlas of mouse mandibular alveolar bone through single-cell RNA sequencing (scRNA-seq). A more active immune microenvironment is identified in alveolar bone, with a higher proportion of mature immune cells than in long bone. Among all immune cell populations, the monocyte/macrophage subpopulation most actively interacts with mesenchymal stem cells (MSCs) subpopulation. Alveolar bone monocytes/macrophages express a higher level of Oncostatin M (Osm) compared to long bone, which promotes osteogenic differentiation and inhibits adipogenic differentiation of MSCs. In summary, our study reveals a unique immune microenvironment of alveolar bone, which may provide a more precise immune-modulatory target for therapeutic treatment of oral diseases.

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Section: Introductionmentioning

confidence: 99%

Mapping the immune microenvironment for mandibular alveolar bone homeostasis at single-cell resolution

Lin

Zhang

et al. 2021

Bone Res

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“…These genes play important roles in osteogenic differentiation and can directly reflect the level of osteogenic differentiation. 42 , 43 For example, Runx2 is associated with the content of basic transcription factors in osteogenic differentiation. Col I is associated with the formation of extracellular matrix.…”

Section: Resultsmentioning

confidence: 99%

Construction of hollow polydopamine nanoparticle based drug sustainable release system and its application in bone regeneration

et al. 2021

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Nanomaterial-based drug sustainable release systems have been tentatively applied to bone regeneration. They, however, still face disadvantages of high toxicity, low biocompatibility, and low drug-load capacity. In view of the low toxicity and high biocompatibility of polymer nanomaterials and the excellent load capacity of hollow nanomaterials with high specific surface area, we evaluated the hollow polydopamine nanoparticles (HPDA NPs), in order to find an optimal system to effectively deliver the osteogenic drugs to improve treatment of bone defect. Data demonstrated that the HPDA NPs synthesized herein could efficiently load four types of osteogenic drugs and the drugs can effectively release from the HPDA NPs for a relatively longer time in vitro and in vivo with low toxicity and high biocompatibility. Results of qRT-PCR, ALP, and alizarin red S staining showed that drugs released from the HPDA NPs could promote osteogenic differentiation and proliferation of rat bone marrow mesenchymal stem cells (rBMSCs) in vitro. Image data from micro-CT and H&E staining showed that all four osteogenic drugs released from the HPDA NPs effectively promoted bone regeneration in the defect of tooth extraction fossa in vivo, especially tacrolimus. These results suggest that the HPDA NPs, the biodegradable hollow polymer nanoparticles with high drug load rate and sustainable release ability, have good prospect to treat the bone defect in future clinical practice.

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“…Bone defects are frequently occurring diseases due to tumor, trauma, infection, fracture, and osteoporosis. Traditional treatments mainly include autograft, allograft bone transplantation, prostheses, and artificial bone replacement [ 82 , 83 ]. However, all these methods suffer from some limitations, such as short duration, immune rejection, low biocompatibility, and poor mechanical properties [ 84 ].…”

Section: Specific Application: Chitosan Alginate Agarose Scaffolmentioning

confidence: 99%

Polysaccharide-Based Aerogel Production for Biomedical Applications: A Comparative Review

2021

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A comparative analysis concerning bio-based gels production, to be used for tissue regeneration, has been performed in this review. These gels are generally applied as scaffolds in the biomedical field, thanks to their morphology, low cytotoxicity, and high biocompatibility. Focusing on the time interval 2015–2020, the production of 3D scaffolds of alginate, chitosan and agarose, for skin and bone regeneration, has mainly been investigated. Traditional techniques are critically reviewed to understand their limitations and how supercritical CO2-assisted processes could overcome these drawbacks. In particular, even if freeze-drying represents the most widespread drying technique used to produce polysaccharide-based cryogels, supercritical CO2-assisted drying effectively allows preservation of the nanoporous aerogel structure and removes the organic solvent used for gel preparation. These characteristics are essential for cell adhesion and proliferation.

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Macrophage-derived small extracellular vesicles promote biomimetic mineralized collagen-mediated endogenous bone regeneration

Cited by 44 publications

References 41 publications

Mapping the immune microenvironment for mandibular alveolar bone homeostasis at single-cell resolution

Mapping the immune microenvironment for mandibular alveolar bone homeostasis at single-cell resolution

Construction of hollow polydopamine nanoparticle based drug sustainable release system and its application in bone regeneration

Polysaccharide-Based Aerogel Production for Biomedical Applications: A Comparative Review

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