2022
DOI: 10.1021/acs.nanolett.2c02560
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Macrophage-Membrane-Camouflaged Nonviral Gene Vectors for the Treatment of Multidrug-Resistant Bacterial Sepsis

Abstract: Sepsis is a life-threatening disease caused by systemic bacterial infections, with high morbidity and mortality worldwide. As the standard treatment for sepsis, antibiotic therapy faces the challenge of impaired macrophages and drug-resistant bacteria. In this study, we developed a membrane-camouflaged metal−organic framework (MOF) system for plasmid DNA (pDNA) delivery to combat sepsis. The antimicrobial gene LL37 was efficiently encapsulated in the pH-sensitive MOF, and the nanoparticles were decorated with … Show more

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Cited by 37 publications
(30 citation statements)
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“…This method facilitated the production of antimicrobial peptides in a continuous fashion. The constructed system significantly increased the survival rates of immunosuppressed septic mice infected with MRSA via effective gene therapy and sequester of inflammatory cytokines (Figure 4; Cao et al, 2022), demonstrating the potential of macrophage membrane coating in gene therapy. Soracha et al reported a biomimetic nanoparticle composed of PLGA core and macrophage membrane surface.…”
Section: White Blood Cell Membrane-coating Nanoparticlesmentioning
confidence: 90%
See 1 more Smart Citation
“…This method facilitated the production of antimicrobial peptides in a continuous fashion. The constructed system significantly increased the survival rates of immunosuppressed septic mice infected with MRSA via effective gene therapy and sequester of inflammatory cytokines (Figure 4; Cao et al, 2022), demonstrating the potential of macrophage membrane coating in gene therapy. Soracha et al reported a biomimetic nanoparticle composed of PLGA core and macrophage membrane surface.…”
Section: White Blood Cell Membrane-coating Nanoparticlesmentioning
confidence: 90%
“…The constructed MMD-LL37 can sequester pro-inflammatory cytokines, realize the targeted delivery of plasmid, generate antibacterial LL37, and eradicate bacteria in circulation and those hidden inside cells. Reproduced with permission (Cao et al, 2022; Copyright© 2022, American Chemical Society). Streptococcus mutans reside in a self-producing matrix of extracellular polymeric substances (EPS).…”
Section: Bacterial Membrane-derived Nanoparticlesmentioning
confidence: 99%
“…Additionally, macrophage-or neutrophil-like biomimetic NPs were also shown to simultaneously absorb PAMPs and proinflammatory cytokines, which implies a more potent effect for sepsis management (Thamphiwatana et al, 2017;Wu et al, 2020). Other leukocytebased biomimetic NPs, nanocarriers, and nanovesicles were demonstrated to enhance inflammation targeting in bacterial sepsis (Zinger et al, 2021;Cao et al, 2022) or otherwise enable anti-inflammation and targeted antiviral treatment in sepsisassociated diseases such as COVID-19 (Tan et al, 2021). Of note, macrophage-membrane-coated polymer NPs (i.e., leukosomes) were shown in a recent work to enhance the anti-inflammatory effect of dexamethasone in a murine endotoxemia model, along with their inherent anti-inflammatory activity due to the contact with macrophages, which skews them into immunosuppressive phenotypes (Molinaro et al, 2020).…”
Section: Anti-inflammationmentioning
confidence: 99%
“…For example, a recent study demonstrated the fabrication of a metal-organic framework encapsulated with a plasmid expressing antimicrobial gene LL37 and coated with macrophage membranes. This type of biomimetic NP boosted the antimicrobial response and conferred protection in septic mice bearing immunodeficiency (Cao et al, 2022). Other researchers described the coating of viral mimicking molecules, which enhanced cross-presentation to ameliorate infectious diseases (Molino et al, 2013).…”
Section: Reversing Immunosuppressionmentioning
confidence: 99%
“…[ 17 ] In addition, the immunocell membrane encapsulation strategy can also enhance the enrichment effect of therapeutics at the infected site. [ 18 ] 4) The diagnostic and antibacterial performances of theranostic nanoplatforms can be activated upon encountering infected tissue. [ 19 ] More specific markers from infected tissue still need to be screened and discovered and can be used as a trigger source for targeted delivery of diagnostic, antibacterial, and theranostic nanoagents.…”
Section: Introductionmentioning
confidence: 99%