Macrophage Migration Inhibitory Factor Levels in Gingival Crevicular Fluid, Saliva, and Serum of Chronic Periodontitis Patients

Ortiz-García, Yveth Marlene; García-Iglesias, Trinidad; Morales-Velazquez, Gabriela; Lazalde-Ramos, Blanca Patricia; Zúñiga‐González, Guillermo Moisés; Ortiz-García, Ramón Guillermo; Zamora‐Pérez, Ana Lourdes

doi:10.1155/2019/7850392

Cited by 14 publications

(19 citation statements)

References 43 publications

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“…Plastin-2 (LCP1) regulates leukocyte adhesion to integrin and has been described among salivary proteins associated with periodontitis in patients with type 2 diabetes mellitus [ 24 ]. Finally, macrophage migration inhibitory factor (MIF), another important effector cytokine of the innate immune system, has been also associated with the disease severity in chronic periodontitis [ 25 ]. Noteworthy, none of our patients presented a clinically evident periodontitis thus we hypothesized that salivary EVs cargo may represent a useful source of early biomarkers able to reflect initial alterations of the gingival crevicular fluid and subclinical abnormalities of the oral cavity.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Extracellular Vesicle Cargo in Sjögren’s Syndrome through a SWATH-MS Proteomics Approach

Finamore¹,

Cecchettini

Ceccherini³

et al. 2021

IJMS

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Primary Sjögren’s syndrome (pSS) is a complex heterogeneous disease characterized by a wide spectrum of glandular and extra-glandular manifestations. In this pilot study, a SWATH-MS approach was used to monitor extracellular vesicles-enriched saliva (EVs) sub-proteome in pSS patients, to compare it with whole saliva (WS) proteome, and assess differential expressed proteins between pSS and healthy control EVs samples. Comparison between EVs and WS led to the characterization of compartment-specific proteins with a moderate degree of overlap. A total of 290 proteins were identified and quantified in EVs from healthy and pSS patients. Among those, 121 proteins were found to be differentially expressed in pSS, 82% were found to be upregulated, and 18% downregulated in pSS samples. The most representative functional pathways associated to the protein networks were related to immune-innate response, including several members of S100 protein family, annexin A2, resistin, serpin peptidase inhibitors, azurocidin, and CD14 monocyte differentiation antigen. Our results highlight the usefulness of EVs for the discovery of novel salivary-omic biomarkers and open novel perspectives in pSS for the identification of proteins of clinical relevance that could be used not only for the disease diagnosis but also to improve patients’ stratification and treatment-monitoring. Data are available via ProteomeXchange with identifier PXD025649.

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Section: Discussionmentioning

confidence: 99%

Characterization of Extracellular Vesicle Cargo in Sjögren’s Syndrome through a SWATH-MS Proteomics Approach

Finamore¹,

Cecchettini

Ceccherini³

et al. 2021

IJMS

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“…Deserves special attention the idea of [6,16] bacterial contamination as a factor of the development of experimental periodontitis, which was proposed in 2015 [14]. There is proposed the concept of an increase in the role of bacterial contamination in patients with periodontitis against the background of a decrease in the expression of antimicrobial peptides in the epithelium of oral cavity, which can further lead to invasion of initiation of chronic systemic inflammation [17,18]. Our study of the spectrum of cytokines showed that the concentration of pro-inflammatory cytokines significantly increases at the 60th and 90th day of the experiment (concentration of IL-1β and TNF-α and anti-inflammatory IL-10 significantly decreased.…”

Section: Discussionmentioning

confidence: 99%

Study of influence of bacterial lysate on cytokine indicators in experimental periodontis in rats

2021

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The aim of the research: to optimize the treatment of patients with a disturbed cytokine profile by a bacterial lysate in an experimental model of periodontitis against the background of changes in the local and systemic immune response. Materials and methods: prospective study has been conducted on experimental periodontitis in 42 rats for 90 days. The animals were treated by solution of «Respibron» and the reference drug «Imudon». The concentration of cytokines in the blood serum was determined by the enzyme immunoassay, circulating immune complexes – precipitation method is measured on a spectrophotometer, C-reactive protein – using a latex diagnosticum. All interventions and euthanasia of animals were carried out in compliance with the European principles. Results: the obtained data from the study indicate a high degree a state of decompensation of experimental periodontitis. An immuno-inflammatory reaction preceding periodontitis is accompanied by the disturbances of the cytokine balance, local and systemic immune response. Applying of bacterial lysates led to the compensation of bacterial dysbiosis, restoration of the balance of the activity of pro- and anti-inflammatory cytokines, and a decrease the hyperactivity of immunological markers. Conclusion: the studies carried out have shown that the use of bacterial lysate is a pathogenetically substantiated component of therapeutic tactics. The normalizing effect of the lysate on the parameters of the immune response and cytokine profile has been reliably shown. Considering the immuno-inflammatory reaction at the experimental periodontitis, accompanied by the cytokine imbalance, study the pharmacological correction by bacterial lysate should continue from the point of view of establishing the mechanisms of development, protection, and restoration of tissues. It has been proved that by the end of the 90-day period of observation of the course of experimental periodontitis, the power of natural compensatory mechanisms acquired a state of decompensation. It has manifested by increased pro-inflammatory cytokine IL-1β in 1.7 times, and TNF-α in 3 times, decreased anti-inflammatory cytokine IL-10 in 1.6 times; increased the concentration of CIC in the blood in 2 times, C-reactive protein in 4.4 times at the local level in the gum tissue homogenate of the control pathology group of animals. The powerful corrective effect of bacterial lysates «Respibron» and «Imudon» on the indicators of the cytokine series on the model of chronic experimental periodontitis has been reliably established. The normalizing effect is due to the compensation of bacterial dysbiosis, restoration of the balance of the activity of pro- and anti-inflammatory cytokines, and a decrease in the hyperactivity of immunological markers. The therapeutic effect of «Respibron» (bacterial lysate of 13 strains of microorganisms) can be assessed as expressive and more powerful in comparison with «Imudon». The obtained results make it possible to continue the study of the course of experimental periodontitis from the point of view of establishing the mechanisms of development, protection, and restoration of tissues under conditions of pharmacological correction with bacterial lysate «Respibron».

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“…Moreover, gene inactivation of MIF receptor (CD74) or the use of MIF agonist ISO-1 resulted in a significant reduction in the proliferation of gastric cancer cells [ 84 ]. Therefore, the higher concentrations of serum and salivary MIF observed in PD might stimulate the proliferation, metastasis and invasion, of oral cancer cells into other tissues [ 85 ].…”

Section: Oscc Immune Permissive Environment Induced By Periodontal Diseasementioning

confidence: 99%

The Crossroads of Periodontitis and Oral Squamous Cell Carcinoma: Immune Implications and Tumor Promoting Capacities

Elebyary

Barbour

Fine

et al. 2021

Front. Oral. Health

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Periodontitis (PD) is increasingly considered to interact with and promote a number of inflammatory diseases, including cancer. In the case of oral squamous cell carcinoma (OSCC) the local inflammatory response associated with PD is capable of triggering altered cellular events that can promote cancer cell invasion and proliferation of existing primary oral carcinomas as well as supporting the seeding of metastatic tumor cells into the gingival tissue giving rise to secondary tumors. Both the immune and stromal components of the periodontium exhibit phenotypic alterations and functional differences during PD that result in a microenvironment that favors cancer progression. The inflammatory milieu in PD is ideal for cancer cell seeding, migration, proliferation and immune escape. Understanding the interactions governing this attenuated anti-tumor immune response is vital to unveil unexplored preventive or therapeutic possibilities. Here we review the many commonalities between the oral-inflammatory microenvironment in PD and oral-inflammatory responses that are associated with OSCC progression, and how these conditions can act to promote and sustain the hallmarks of cancer.

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Macrophage Migration Inhibitory Factor Levels in Gingival Crevicular Fluid, Saliva, and Serum of Chronic Periodontitis Patients

Cited by 14 publications

References 43 publications

Characterization of Extracellular Vesicle Cargo in Sjögren’s Syndrome through a SWATH-MS Proteomics Approach

Characterization of Extracellular Vesicle Cargo in Sjögren’s Syndrome through a SWATH-MS Proteomics Approach

Study of influence of bacterial lysate on cytokine indicators in experimental periodontis in rats

The Crossroads of Periodontitis and Oral Squamous Cell Carcinoma: Immune Implications and Tumor Promoting Capacities

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