Macrophage/particle interactions: Effect of size, composition and surface area

Shanbhag, Arun S.; Jacobs, Joshua J.; Black, Jonathan; Galante, Jorge O.; Glant, Tibor T.

doi:10.1002/jbm.820280111

Cited by 389 publications

(246 citation statements)

References 23 publications

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“…Our study extends these results and shows for the first time that not only TiAlV-, mixed-, or PMMA-particles [35,40], but also polyethylene-particles induce an activation of NF-KB signalling and the TNFa-promoter. Numerous studies of wear debris from periprosthetic tissues have underlined the key role of PE-particles demonstrating that 70-90% of the recovered particles are submicrometer polyethylene-particles [12, 24,34,46,47]. Metal-on-polyethylene and ceramic-on-polyethylene components are the most common bearing surfaces used in joint replacement leading predominantly to generation of polyethylene-particles.…”

Section: Discussionmentioning

confidence: 99%

Activation of NF-KB signalling and TNFα-expression in THP-1 macrophages by TiAlV- and polyethylene-wear particles

et al. 2005

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Wear particles are believed to induce periprosthetic inflammation which contributes to periprosthetic osteolysis. TNFu plays a pivotal role in the pathogenesis of this process. The molecular mechanisms leading to the development of periprosthetic inflammation with upregulated TNFa expression in monocytic cells in response to different wear particles have yet to be defined. 1n:this study we evaluated the effects of polyethylene-and TiAIV-particles on activation of NF-KB signalling pathways and TNFa biosynthesis and release in monocytic cells with respect to periprosthetic osteoclastogenesis. THP-1 monocytic cells were differentiated to macrophage-like cells and exposed to LPS-detoxified polyethylene and prosthesis-derived TiAlV-particles. TNFa release was analyzed in culture supernatant by ELISA. NF-KB activation was examined by electrophoretic mobility shift assay (EMSA), and NF-KB target promoter activities including transactivation of the TNFa promoter were determined by luciferase reporter gene assays. Differentiated THP-1 macrophages were exposed to increasing numbers of particles for 0, 60, 180 and 360 min. Both, polyethylene-and TiAIV-particles induced a significant activation of both NF-KB and TNFa promoters at 180 min. A significant TNFu release was detected after 360 min exposure to polyethylene-and TiAlV-particles in a dose dependent manner. In comparison, LPS induced a much greater activation of NF-KB and TNFa promoters, and TNFa secretion into the supernatant was strongly induced. These results provide evidence that induction of the NF-KB signal transduction pathway in macrophages plays a major role in initiating and mediating the inflammatory response leading to periprosthetic osteolysis.

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Section: Discussionmentioning

confidence: 99%

Activation of NF-KB signalling and TNFα-expression in THP-1 macrophages by TiAlV- and polyethylene-wear particles

et al. 2005

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“…Previous studies demonstrated that cells were sensitive to the surface of material present in the cell culture [10,[24][25].…”

Section: Particles Characteristicsmentioning

confidence: 99%

MMP-2, MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles

Laquerrière

Grandjean-Laquerriere²,

Addadi-Rebbah

et al. 2004

Biomaterials

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After calcium-phosphates biomaterials based implantation like hydroxyapatite coating, particles are released in the periprosthetic tissues. Fibrous tissue around wear debris are mainly made of monocytes that can be produced metalloprotease (MMP), considered to be the key enzymes in extracellular matrix turnover. Tissue inhibitors of metalloproteinases are important regulator of MMPs activity. Interleukin-1 mainly produced by monocytes can also regulate MMPs production. In the present work, we have evaluated the effect of hydroxyapatite particles characteristics (size, shape and sintering temperature) on the MMP-2, -9 and their inhibitors TIMP-2, -1 production. Our results demonstrate that sintering temperature (that modify crystal size and surface area) have few effect on MMPs and TIMPs production. Not-phagocytable particles induced more MMP-9, altough phagocytable particles induced more IL-1β production. The shape of the particles was the most important factor since needle particles induced the most important quantity of MMPs and IL-1β.

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“…To date, methods developed to assess the biologic reactivity to wear debris have considered the sizes, compositions, and surface areas of the particles [22,30,33,44,45]. However, a careful reevaluation of the biologic activity for crosslinked PE should take into account the overall volume in addition to the particle size, composition, and area and should be based on a highly precise method for obtaining purified and well-characterized PE particles.…”

Section: Introductionmentioning

confidence: 99%

The John Charnley Award: An Accurate and Sensitive Method to Separate, Display, and Characterize Wear Debris: Part 1: Polyethylene Particles

Billi

Benya

Kavanaugh

et al. 2012

Clinical Orthopaedics &Amp; Related Research

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Background Numerous studies indicate highly crosslinked polyethylenes reduce the wear debris volume generated by hip arthroplasty acetabular liners. This, in turns, requires new methods to isolate and characterize them. Questions/purposes We describe a method for extracting polyethylene wear particles from bovine serum typically used in wear tests and for characterizing their size, distribution, and morphology.Methods Serum proteins were completely digested using an optimized enzymatic digestion method that prevented the loss of the smallest particles and minimized their clumping. Density-gradient ultracentrifugation was designed to remove contaminants and recover the particles without filtration, depositing them directly onto a silicon wafer. This provided uniform distribution of the particles and high contrast against the background, facilitating accurate, automated, morphometric image analysis. The accuracy and precision of the new protocol were assessed by recovering and characterizing particles from wear tests of three types of polyethylene acetabular cups (no crosslinking and 5 Mrads and 7.5 Mrads of gamma irradiation crosslinking). Results The new method demonstrated important differences in the particle size distributions and morphologic parameters among the three types of polyethylene that could not be detected using prior isolation methods. Conclusion The new protocol overcomes a number of limitations, such as loss of nanometer-sized particles and artifactual clumping, among others. Clinical Relevance The analysis of polyethylene wear particles produced in joint simulator wear tests of prosthetic joints is a key tool to identify the wear mechanisms that produce the particles and predict and evaluate their effects on periprosthetic tissues.

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Macrophage/particle interactions: Effect of size, composition and surface area

Cited by 389 publications

References 23 publications

Activation of NF-KB signalling and TNFα-expression in THP-1 macrophages by TiAlV- and polyethylene-wear particles

Activation of NF-KB signalling and TNFα-expression in THP-1 macrophages by TiAlV- and polyethylene-wear particles

MMP-2, MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles

The John Charnley Award: An Accurate and Sensitive Method to Separate, Display, and Characterize Wear Debris: Part 1: Polyethylene Particles

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