Macrophage phagocytosis of wound neutrophils

Abstract

Resolution of acute inflammation is thought to require the recognition and phagocytosis of apoptotic neutrophils (PMN) through receptorligand interactions with macrophages (M ). This hypothesis was tested in rat wounds by quantifying apoptosis in freshly harvested and aged-in-culture PMN taken from wounds 1-3 days after injury and by using these wound PMN as phagocytic targets for wound, immune-activated peritoneal, and resident peritoneal M . Less than 6% of freshly harvested PMN exhibited characteristics of apoptosis. On aging in culture, day 1 PMN did not undergo apoptosis, whereas 41 ؎ 1 and 29 ؎ 1% of day 2 and 3 PMN, respectively, developed apoptosis, which corresponded to increased ingestion by M .

Macrophages in Kidney Repair and Regeneration

“…In human populations, the use of Genome Wide Association Screening (GWAS) has identified a number of loci associated with CKD. 1,2 Further refinements to GWAS techniques using admixture-mapping linkage disequilibrium also allowed for the identification of a locus on chromosome 22q12 that predisposes individuals of African descent to nondiabetic glomerular injury, 3,4 and recent analyses strongly indicate that the affected gene is ApoL1. 5,6 Undoubtedly, other genes are involved in development of progressive kidney disease.…”

“…Macrophages are particularly adept at clearing debris, extracellular matrix, immune complexes, and dead cell products of tissue injury and most of the time perform such tasks silently. 3 The same sort of macrophages also seem to have the capacity to release almost every cytokine and growth factor described in the literature. Coordinated release of many of these factors promotes organizedtissueregenerationincludingbasementmembranesynthesis, cell proliferation, cell migration, and dampening of the inflammatory response.…”

A molecular profile of the mouse gastric parietal cell with and without exposure to Helicobacter pylori

“…VEGF-B stimulates angiogenesis in growing tissues and could help PCs maintain blood supply in isthmal and other regions of gastric units. CD36 is a membrane-bound lipid scavenger that functions in cell-cell communication and phagocytosis of apoptotic bodies (29,30).…”

Gene transfer into inflamed glomeruli using macrophages transfected with adenovirus

“…3 Although responsible for injury in models of inflammation, macrophages are also crucial to resolution of injury and for tissue re-modeling and repair. The processes that control these events are increasingly being understood: (1) macrophage infiltration is required for appropriate wound repair; [4][5][6] (2) macrophage uptake of apoptotic infiltrating neutrophils, lymphocytes and eosinophils is important in resolution of pulmonary inflammation in animal models 7,8 and human disease; 9,10 to localise to inflamed glomeruli after direct injection into the renal artery of rats with an experimentally induced glomerular inflammation caused by nephrotoxic nephritis. The injection of transfected macrophages reduces the severity of injury in this model of glomerulonephritis as shown by a reduction in the degree of albuminuria.…”