Macrophage polarity and wound age determination

Kuninaka, Yumi; Ishida, Yuko; Ishigami, Akiko; Nosaka, Mizuho; Matsuki, Jumpei; Yasuda, Haruki; Kofuna, Ayumi; Kimura, Akihiko; Furukawa, Fukumi; Kondo, Toshikazu

doi:10.1038/s41598-022-24577-9

Cited by 20 publications

(9 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The balance between M1 and M2 macrophage during wound healing was quite important for accelerated wound repair 68–70 . At the beginning of wound healing, M1 macrophage was quickly augmented and dominated in the wound bed, which contributed to the inflammation in the wound bed to remove infections and pathogens.…”

Section: Resultsmentioning

confidence: 99%

“…The balance between M1 and M2 macrophage during wound healing was quite important for accelerated wound repair. [68][69][70] At the beginning of wound healing, M1 macrophage was quickly augmented and dominated in the wound bed, which contributed to the In the repairing stage, M1 macrophage decreased while M2 macrophage significantly increased to accelerate wound healing and remodeling. Therefore, the macrophage polarization and balance were further studied to investigate the healing effect of TA/PVA fiber through the immunofluorescence staining of CD86 and CD206, which were the typical marker of M1 and M2, respectively.…”

Section: Ta/pva Fiber Attenuates Inflammationmentioning

confidence: 99%

See 1 more Smart Citation

Drug cross‐linking electrospun fiber for effective infected wound healing

Luo

Zheng

Wang

et al. 2023

Bioengineering & Transla Med

View full text Add to dashboard Cite

The management of infected wounds is still an intractable challenge in clinic. Development of antibacterial wound dressing is of great practical significance for wound management. Herein, a natural‐derived antibacterial drug, tannic acid (TA), was incorporated into the electrospun polyvinyl alcohol (PVA) fiber (TA/PVA fiber, 952 ± 40 nm in diameter). TA worked as a cross‐linker via hydrogen bonding with PVA to improve the physicochemical properties of the fiber and to reach a sustained drug release (88% release of drug at 48 h). Improved mechanical property (0.8–1.2 MPa) and computational simulation validated the formation of the hydrogen bonds between TA and PVA. Moreover, the antibacterial and anti‐inflammatory characteristics of TA laid the foundation for the application of TA/PVA fiber in repairing infected wounds. Meanwhile, in vitro studies proved the high hemocompatibility and cytocompatibility of TA/PVA fiber. Further in vivo animal investigation showed that the TA/PVA fiber promoted the repair of infected wound by inhibiting the bacterial growth, promoting granulation formation, and collagen matrix deposition, accelerating angiogenesis, and inducing M2 macrophage polarization within 14 days. All the data demonstrated that the TA cross‐linked fiber would be a potent dressing for bacteria‐infected wound healing.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Ta/pva Fiber Attenuates Inflammationmentioning

confidence: 99%

Drug cross‐linking electrospun fiber for effective infected wound healing

Luo

Zheng

Wang

et al. 2023

Bioengineering & Transla Med

View full text Add to dashboard Cite

show abstract

“…Within a chronic inflammatory microenvironment, pro-inflammatory M1 macrophages take on a central role as the primary generators of inflammatory cytokines (such as IL-6 and IL-1β) [ 66 , 67 , 68 ]. The balance between M1 and M2 macrophages in skin wounds offers valuable insights into determining the age of the wound [ 69 ]. During the initial 1 to 7 days after injury, the population of M1 macrophages predominates.…”

Section: Discussionmentioning

confidence: 99%

“…Dr. Wang’s research highlighted distinct distributions of M1 and M2 macrophages in the reparative area of non-traumatic osteonecrosis of the femoral head [ 70 ]. Notably, M2 macrophages exhibited accumulation around perivascular regions (with an approximate M1/M2 ratio of 0.05), while M1 macrophages were predominantly present in avascular zones (with an approximate M1/M2 ratio of 12) [ 69 ]. In our future studies, we will aim to delve more deeply into the precise distribution and roles of individual M1 and M2 macrophages, as well as the M1/M2 ratio.…”

Section: Discussionmentioning

confidence: 99%

Anatomical Anal Stenosis after PPH: Insights from a Retrospective Study and Rat Model

Wen,

Huang,

Wang

2024

IJMS

View full text Add to dashboard Cite

High-grade hemorrhoids are usually recommended to receive operational treatments. However, these traditional surgeries are associated with severe postoperative pain. A procedure for prolapse and hemorrhoids (PPH), a circular staple device, has been developed to improve short-term outcomes, including reducing the severity of postoperative pain. PPH, compared to conventional surgery, has been associated with the incidence of anatomical anal stenosis. The causes of stenosis after PPH are not yet clear. We first analyzed the complications of our patients with PPH, and then developed a rat model to verify the tension force of PPH using Hematoxylin-eosin, Masson’s trichrome, immunohistochemistry, and immunofluorescence staining. Our clinical data showed that PPH significantly improved postoperative pain, but that it resulted in higher incidences of complications, including anal stenosis, than hemorrhoidectomy. We simulated the status of PPH and developed a rat model to verify PPH’s tension force, including the scarring area and the deposition of proinflammatory factors, angiogenic factors, and fibrotic factors. The tension wound histological data showed more extensive granulation tissue and inflammatory cell infiltration and a thicker epidermis than the control group on day 12 post-operation and tension treatment. In addition to IL-1β and IL-10 cytokines on day 3 and IL-1β, IL-6, and IL-10 cytokines on day 12 post-operation in the tension group, two angiogenic factors, CD31 and VEGF-A, were found to have a more significant expression on day 7 post-operation in the tension group. The mean scar area was larger and the distribution of fibrotic proteins (collagen 1, α-SMA, CTGF, and MMP2) in the tension group was significantly broader than in the control on day 12 post-operation and tension treatment. Based on the findings of our animal model, the development of a lesser tensile force for PPH to decrease the deposition of proinflammatory factors, angiogenic factors, and fibrotic factors is urgently required.

show abstract

“…48 On the other hand, in Mus , both M1 and M2 macrophages are present throughout all connective tissues of the injured area. 53 Interestingly, the depletion of macrophages through clodronate liposome injections severely delayed ear hole closure in Acomys. 50 The neutrophil response in Acomys is delayed compared to Mus and differences were found in the number of neutrophils in various compartments, such as blood and bone marrow, when comparing several species of Acomys to Mus.…”

Section: Acomys African Spiny Mouse a Mammal Capable Of Regenerationmentioning

confidence: 99%