Macrophage polarization in THP-1 cell line and primary monocytes: A systematic review

Yasin, Zahidah Nasuha Mohd; Idrus, F.N. Mohd; Hoe, Chee Hock; Yvonne-Tee, Get Bee

doi:10.1016/j.diff.2022.10.001

Cited by 51 publications

(21 citation statements)

References 96 publications

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“…In different microenvironments, macrophages are polarized into two subtypes: classically activated macrophages (M1) or alternatively activated macrophages (M2) [ 23 , 24 ]. We used the human monocytic cell line THP-1, which is useful for studying monocyte/macrophage differentiation [ 25 , 26 ], and examined the expression levels of Rab44 compared with M1 markers, including IL-6, CD80, and NOS2, and M2 markers, including interleukin-10 (IL-10), CD206, and arginase. A real-time PCR analysis of mRNA levels revealed that Rab44 was transiently upregulated at an early phase in both M1 ( Figure 1 a, left panel and b) and M2 differentiation ( Figure 1 a, right panel and b).…”

Section: Resultsmentioning

confidence: 99%

Rab44 Deficiency Induces Impaired Immune Responses to Nickel Allergy

Noguromi

Yamaguchi²,

Satô³

et al. 2023

IJMS

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Rab44 was recently identified as an atypical Rab GTPase that possesses EF-hand and coiled-coil domains at the N-terminus, and a Rab-GTPase domain at the C-terminus. Rab44 is highly expressed in immune-related cells such as mast cells, macrophages, osteoclasts, and granulocyte-lineage cells in the bone marrow. Therefore, it is speculated that Rab44 is involved in the inflammation and differentiation of immune cells. However, little is known about the role of Rab44 in inflammation. In this study, we showed that Rab44 was upregulated during the early phase of differentiation of M1- and M2-type macrophages. Rab44-deficient mice exhibited impaired tumor necrosis factor alpha and interleukin-10 production after lipopolysaccharide (LPS) stimulation. The number of granulocytes in Rab44-deficient mice was lower, but the lymphocyte count in Rab44-deficient mice was significantly higher than that in wild-type mice after LPS stimulation. Moreover, Rab44-deficient macrophages showed impaired nickel-induced toxicity, and Rab44-deficient mice showed impaired nickel-induced hypersensitivity. Upon nickel hypersensitivity induction, Rab44-deficient mice showed different frequencies of immune cells in the blood and ears. Thus, it is likely that Rab44 is implicated in immune cell differentiation and inflammation, and Rab44 deficiency induces impaired immune responses to nickel allergies.

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Section: Resultsmentioning

confidence: 99%

Rab44 Deficiency Induces Impaired Immune Responses to Nickel Allergy

Noguromi

Yamaguchi²,

Satô³

et al. 2023

IJMS

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“…This human cell line has been widely used in similar studies as indicated above, and is widely used in cellular inflammation studies with similar functionality to human monocytes/macrophages. This cell line has several advantages, including: i) a more homogenous genetic background; ii) the ability to grow in unlimited passages providing high yield of cells; and iii) the ability to be transfected with non‐viral systems (Mohd Yasin et al., 2022 ). We used two concentrations of EVs as previously described (Trelis et al., 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Extracellular vesicles from the trematodes Fasciola hepatica and Dicrocoelium dendriticum trigger different responses in human THP‐1 macrophages

Sánchez-López

González-Arce

Soler

et al. 2023

J of Extracellular Vesicle

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Extracellular vesicles (EVs) released by the helminths Dicrocoelium dendriticum and Fasciola hepatica are important modulators of the host immune response, contributing to the establishment of the infection. Monocytes and, in particular, macrophages are major regulators of the inflammatory response and are likely responsible for the phagocytosis of most of the parasite EVs. In this study, we isolated EVs from F. hepatica (FhEVs) and D. dendriticum (DdEVs) by size exclusion chromatography (SEC) and characterized them by nanoparticle tracking analysis, transmission electron microscopy and LC-MS/MS, and analyzed the cohort of proteins. The treatment of monocytes/macrophages with FhEVs, DdEVs or EV-depleted fractions from SEC, demonstrated species-specific effects of the EVs. In particular, FhEVs reduce the migratory capacity of monocytes and the analysis of the cytokine profile showed that they induce a mixed M1/M2 response, exerting anti-inflammatory properties in Lipopolysaccharide-activated macrophages. In contrast, DdEVs do not affect monocyte migration and seem to have pro-inflammatory properties. These results correlate with the differences in the life cycle of both parasites, suggesting different host immune responses. Only F. hepatica migrates to the bile duct through the liver parenchyma, driving the host immune response to heal deep erosions. Furthermore, the proteomic analysis of the macrophages upon FhEV treatment identified several proteins that might be involved in FhEV-macrophage interactions.

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“…Alternatively, macrophages derived from the human monocytic cell line THP‐1 were extensively used to study the functions and mechanisms of human primary macrophages. [ 52 ] Herein, THP‐1 cells were employed to investigate the immunostimulatory effect of Mn@BSA NCs and their NWs. Initially, THP‐1 cells were differentiated into resting macrophages (M0) using phorbol‐12‐myristate‐13‐acetate (PMA) at 100 ng mL −1 for 48 h, accompanied by a macrophage‐like morphology.…”

Section: Resultsmentioning

confidence: 99%

Manganese@Albumin Nanocomplex and Its Assembled Nanowire Activate TLR4‐Dependent Signaling Cascades of Macrophages

Huang,

Gao,

et al. 2023

Advanced Materials

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The immunomodulatory effect of divalent manganese cations (Mn2+), such as activation of the cGAS−STING pathway or NLRP3 inflammasomes, positions them as adjuvants for cancer immunotherapy. In this study, it is found that trace Mn2+ ions, bound to bovine serum albumin (BSA) to form Mn@BSA nanocomplexes, stimulate pro‐inflammatory responses in human‐ or murine‐derived macrophages through TLR4‐mediated signaling cascades. Building on this, the assembly of Mn@BSA nanocomplexes to obtain nanowire structures enables stronger and longer‐lasting immunostimulation of macrophages by regulating phagocytosis. Furthermore, Mn@BSA nanocomplexes and their nanowires efficiently activate peritoneal macrophages, reprogramme tumor‐associated macrophages, and inhibit the growth of melanoma tumors in vivo. They also show better biosafety for potential clinical applications compared to typical TLR4 agonists such as lipopolysaccharides. Accordingly, the findings provide insights into the mechanism of metalloalbumin complexes as potential TLR agonists that activate macrophage polarization and highlight the importance of their nanostructures in regulating macrophage‐mediated innate immunity.

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Macrophage polarization in THP-1 cell line and primary monocytes: A systematic review

Cited by 51 publications

References 96 publications

Rab44 Deficiency Induces Impaired Immune Responses to Nickel Allergy

Rab44 Deficiency Induces Impaired Immune Responses to Nickel Allergy

Extracellular vesicles from the trematodes Fasciola hepatica and Dicrocoelium dendriticum trigger different responses in human THP‐1 macrophages

Manganese@Albumin Nanocomplex and Its Assembled Nanowire Activate TLR4‐Dependent Signaling Cascades of Macrophages

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