Macrophage prostaglandin E2 and oxidative responses to endotoxin during immunosuppression associated with anaesthesia and transfusion

Ross, William; Leaver, H.A.; Yap, Peng Lee; Raab, Gillian M.; Su, Bo; Carter, D.C.; Mao, Jian‐Hua; Wu, Qian; Prescott, R. J.

doi:10.1016/0952-3278(93)90180-5

Cited by 8 publications

(5 citation statements)

References 27 publications

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“…I t has been suggested that intracellular sequestration limits dichlorofluorescein availability and reactivity [40]. However, comparison of the dichlorofluorescein probe with other indicators of cellular oxidative activity do not indicate large inaccessible pools of unoxidized probe [26,28,38]. In accord with previous studies using human granulocytes [11,13] we detected a steady rate of 2/,7/-dichlorofluorescein oxidation in unstimulated human monocytes and human umbilical vein derived endothelial cells [23].…”

Section: Discussionsupporting

confidence: 88%

Peroxides in human leucocytes in acute septic shock: a preliminary study of acute phase changes and mortality

Leaver

Yap

Rogers

et al. 1995

Eur J Clin Investigation

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Peroxidation by peripheral blood leucocytes was measured in 15 patients in acute septic shock and 15 uninfected controls, using the probe dichloroflorescein. Mortality in septic subjects was 40%. In 14 of 15 patients from whom serial samples were analysed, periods of increased oxidative activity were detected. Increased peroxidation occurred early in the sequence of clinical changes, at the same time as increases in temperature, blood pressure and C-reactive protein. Peak peroxide production preceded increases in acute phase reactants and changes in leucocyte distribution. Mean peroxide production in leukocytes from patients who died was significantly higher (P < 0.001) than paired controls, and greater (P < 0.05) than peroxide production in patients who survived. The in vitro oxidative response to endotoxin was upregulated in infected patients. This supports the hypothesis that systemic mediators and leucocyte-derived reactive oxygen are involved in the vascular and organ damage associated with fatal septic shock.

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Section: Discussionsupporting

confidence: 88%

Peroxides in human leucocytes in acute septic shock: a preliminary study of acute phase changes and mortality

Leaver

Yap

Rogers

et al. 1995

Eur J Clin Investigation

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“…It has been proposed that the underlying mechanism is due to a post‐operative reduction in circulating T lymphocytes reactive to monocytes, rather than to impaired capacity of individual T cells to respond to activation stimuli. Surgery is also associated with an increase in phagocyte secretion of prostaglandin E 2 (PGE 2 ),which has local immunosuppressive activity [ 34, 45]. Lymphocyte responses such as the mixed lymphocyte reaction are inhibited by local PGE 2 production [ 46] and PGE 2 inhibits spleen cell cytotoxicity in a Lewis lung carcinoma model [ 47].…”

Section: Discussionmentioning

confidence: 99%

Lymphocyte responses following open and minimally invasive thoracic surgery

Craig

et al. 2000

Eur J Clin Investigation

136

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“…Prostaglandin E 2 production by recipient monocytes is increased after transfusion and may induce suppression by activated suppressor cells or by direct suppression of IL-2 [31, 32]. Interestingly, a humoral factor dependent on duration of blood storage, seems to be involved in this mechanism [33]. …”

Section: Discussionmentioning

confidence: 99%

Immunomodulating Effect of Blood Transfusion: Is Storage Time Important?

Mynster¹,

Dybkjær²,

Kronborg³

et al. 1998

Vox Sanguinis

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Objectives: TNF-α and IL-2 are important cytokines in macrophage and T-lymphocyte activity against infection and dissemination of malignant cells. We studied the influence of supernatants from stored whole blood and buffy-coat-depleted SAGM (saline, adenine, glucose and mannitol) blood in stimulating TNF-α and IL-2 release in an ex vivo assay. Methods: Supernatants of 10 units of whole blood and 10 units of SAGM blood were collected after 1, 21 and 35 days of standard blood bank storage. Heparinized blood from 20 healthy volunteers (as ‘recipients’), corresponding in ABO and Rh type to the stored blood, were used in a culture system with LPS and PHA as stimulators of TNF-α and IL-2 release. The effect of added supernatants, from either stored whole blood or SAGM blood, on cytokine release was evaluated compared to saline as control. TNF-α concentration was analyzed by ELISA after culture for 24 h and IL-2 after 72 h, respectively. Results: Supernatants from both stored whole blood and SAGM blood showed a significant decrease in both LPS- and PHA-stimulated TNF-α release that was dependent on storage time. IL-2 was not detected in response to LPS stimulation. PHA-stimulated IL-2 release was significantly reduced and related to storage time of both whole blood and SAGM blood. Conclusions: Recipient cytokine release induced by blood transfusion seems to be dependent on storage time. This may have implications in transfusion-induced immune modulation.

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Macrophage prostaglandin E2 and oxidative responses to endotoxin during immunosuppression associated with anaesthesia and transfusion

Cited by 8 publications

References 27 publications

Peroxides in human leucocytes in acute septic shock: a preliminary study of acute phase changes and mortality

Peroxides in human leucocytes in acute septic shock: a preliminary study of acute phase changes and mortality

Lymphocyte responses following open and minimally invasive thoracic surgery

Immunomodulating Effect of Blood Transfusion: Is Storage Time Important?

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