Macrophage reaction against biomaterials in the mouse model – Phenotypes, functions and markers

Klopfleisch, Robert

doi:10.1016/j.actbio.2016.07.003

Cited by 256 publications

(212 citation statements)

References 130 publications

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“…Additionally, biomaterial‐induced MNGCs share morphological and biological properties with the pathological inflammatory giant cells observed in sarcoidosis and tuberculosis that are similarly formed in response to persistent chronic inflammation . Consequently, proinflammatory MNGCs and macrophages contribute to biomaterial degradation and enhance vascularization . The histomorphometrical results of the vascularization rate underlined this statement.…”

Section: Discussionmentioning

confidence: 81%

Biomaterial‐induced multinucleated giant cells express proinflammatory signaling molecules: A histological study in humans

Zhang

Al‐Maawi

Wang

et al. 2018

J Biomedical Materials Res

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The biomaterials physicochemical characteristics influence their cellular reaction, degradation and regenerative capacities. Macrophages and multinucleated giant cells (MNGCs) are observed in the augmentation area of biomaterials. This study, for the first time, evaluated the polarization pattern of macrophages and MNGCs in response to two different bone substitute materials (synthetic bone substitute material [SBSM] = NanoBone vs. xenogeneic bone substitute material [XBSM] = Bio‐Oss) in human bone biopsies compared to non‐augmented bone (control). Histomorphometrical analysis of the polarization in proinflammatory (M1) and anti‐inflammatory (M2) cells was performed using different immunohistochemical markers: CD‐68 = macrophages; CCR‐7 and Cox‐2 (M1) and CD‐206 and CD‐163 (M2) and tartrate‐resistant acid phosphatase (TRAP). The macrophage polarization pattern in SBSM showed a significantly higher number of M1 cells than did XBSM and non‐augmented bone. XBSM induced a significantly higher number of CD‐206‐positive macrophages than SBSM did. No significant difference was found between XBSM and the non‐augmented bone. MNGCs expressed CD‐68 and TRAP. In both test‐groups, MNGCs showed a high proinflammatory character (CCR‐7 and Cox‐2‐positive) and their number in the SBSM group was significantly higher than that of XBSM. The tissue distribution showed a significantly low percentage of the remaining biomaterial in SBSM compared to XBSM. Within the limitations of this study, these findings show that MNGCs exhibit a rather proinflammatory character and lead to biomaterial degradation, once they are induced in a high number. The premature degradation of bone substitute materials is compensated with a high percentage of connective tissue and not new bone formation. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 780–790, 2019.

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Section: Discussionmentioning

confidence: 81%

Biomaterial‐induced multinucleated giant cells express proinflammatory signaling molecules: A histological study in humans

Zhang

Al‐Maawi

Wang

et al. 2018

J Biomedical Materials Res

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“…Macrophages play a key role to stimulate fibroblasts in the formation of granulation tissue and fibrosis, and their role in foreign body reactions is a very actual topic [32]. While macrophage presence and key role seem evident, different subtypes and reactions might be causal to the actual formation of capsular contracture.…”

Section: Discussionmentioning

confidence: 99%

The Histological Composition of Capsular Contracture Focussed on the Inner Layer of the Capsule: An Intra-Donor Baker-I Versus Baker-IV Comparison

et al. 2018

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“…Surface properties of biomaterials have been shown to influence the phenotype of macrophages, that are key modulators in the foreign body and wound healing responses . For promotion of bone repair, an increase in anti‐inflammatory macrophages following an initial phase with pre‐dominantly pro‐inflammatory macrophages has been suggested to be important .…”

Section: Introductionmentioning

confidence: 99%

Biphasic calcium phosphate with submicron surface topography in an Ovine model of instrumented posterolateral spinal fusion

Dijk

Duan

Luo³

et al. 2018

JOR Spine

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As spinal fusions require large volumes of bone graft, different bone graft substitutes are being investigated as alternatives. A subclass of calcium phosphate materials with submicron surface topography has been shown to be a highly effective bone graft substitute. In this work, a commercially available biphasic calcium phosphate (BCP) with submicron surface topography (MagnetOs; Kuros Biosciences BV) was evaluated in an Ovine model of instrumented posterolateral fusion. The material was implanted stand‐alone, either as granules (BCP granules ) or as granules embedded within a fast‐resorbing polymeric carrier (BCP putty ) and compared to autograft bone (AG). Twenty‐five adult, female Merino sheep underwent posterolateral fusion at L2‐3 and L4‐5 levels with instrumentation. After 6, 12, and 26 weeks, outcomes were evaluated by manual palpation, range of motion (ROM) testing, micro‐computed tomography, histology and histomorphometry. Fusion assessment by manual palpation 12 weeks after implantation revealed 100% fusion rates in all treatment groups. The three treatment groups showed a significant decrease in lateral bending at the fusion levels at 12 weeks ( P < 0.05) and 26 weeks ( P < 0.001) compared to the 6 week time‐point. Flexion‐extension and axial rotation were also reduced over time, but statistical significance was only reached in flexion‐extension for AG and BCP putty between the 6 and 26 week time‐points ( P < 0.05). No significant differences in ROM were observed between the treatment groups at any of the time‐points investigated. Histological assessment at 12 weeks showed fusion rates of 75%, 92%, and 83% for AG, BCP granules and BCP putty , respectively. The fusion rates were further increased 26 weeks postimplantation. Similar trends of bone growth were observed by histomorphometry. The fusion mass consisted of at least 55% bone for all treatment groups 26 weeks after implantation. These results suggest that this BCP with submicron surface topography, in granules or putty form, is a promising alternative to autograft for spinal fusion.

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Macrophage reaction against biomaterials in the mouse model – Phenotypes, functions and markers

Cited by 256 publications

References 130 publications

Biomaterial‐induced multinucleated giant cells express proinflammatory signaling molecules: A histological study in humans

Biomaterial‐induced multinucleated giant cells express proinflammatory signaling molecules: A histological study in humans

The Histological Composition of Capsular Contracture Focussed on the Inner Layer of the Capsule: An Intra-Donor Baker-I Versus Baker-IV Comparison

Biphasic calcium phosphate with submicron surface topography in an Ovine model of instrumented posterolateral spinal fusion

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