Macrophage uptake of cylindrical microparticles investigated with correlative microscopy

Tscheka, Clemens; Hittinger, Marius; Lehr, Claus‐Michael; Schneider-Daum, Nicole; Schneider, Marc

doi:10.1016/j.ejpb.2015.03.010

Cited by 19 publications

(19 citation statements)

References 10 publications

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“…For example, Sindbis virus exit pathway [19] and macrophage uptake of cylindrical microparticles [25] were investigated by CLEM. These studies were performed without causing damages to the ultrastructure of the cell and tissue.…”

Section: Discussionmentioning

confidence: 99%

Visualization of Neutrophil Extracellular Traps and Fibrin Meshwork in Human Fibrinopurulent Inflammatory Lesions: III. Correlative Light and Electron Microscopic Study

Onouchi

Shiogama

Mizutani

et al. 2016

Acta Histochemica et Cytochemica

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Neutrophil extracellular traps (NETs) released from dead neutrophils at the site of inflammation represent webs of neutrophilic DNA stretches dotted with granule-derived antimicrobial proteins, including lactoferrin, and play important roles in innate immunity against microbial infection. We have shown the coexistence of NETs and fibrin meshwork in varied fibrinopurulent inflammatory lesions at both light and electron microscopic levels. In the present study, correlative light and electron microscopy (CLEM) employing confocal laser scanning microscopy and scanning electron microscopy was performed to bridge light and electron microscopic images of NETs and fibrin fibrils in formalin-fixed, paraffin-embedded, autopsied lung sections of legionnaire’s pneumonia. Lactoferrin immunoreactivity and 4'-6-diamidino-2-phenylindole (DAPI) reactivity were used as markers of NETs, and fibrin was probed by fibrinogen gamma chain. Of note is that NETs light microscopically represented as lactoferrin and DAPI-colocalized dots, 2.5 μm in diameter. CLEM gave super-resolution images of NETs and fibrin fibrils: “Dotted” NETs were ultrastructurally composed of fine filaments and masses of 58 nm-sized globular materials. A fibrin fibril consisted of clusters of smooth-surfaced filaments. NETs filaments (26 nm in diameter) were significantly thinner than fibrin filaments (295 nm in diameter). Of note is that CLEM was applicable to formalin-fixed, paraffin-embedded sections of autopsy material.

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Section: Discussionmentioning

confidence: 99%

Visualization of Neutrophil Extracellular Traps and Fibrin Meshwork in Human Fibrinopurulent Inflammatory Lesions: III. Correlative Light and Electron Microscopic Study

Onouchi

Shiogama

Mizutani

et al. 2016

Acta Histochemica et Cytochemica

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“…The correlation was performed by using Shuttle & Find™ (S&F; Zeiss, Jena, Germany). S&F includes a sample holder and a software package that permits the combination of different microscopes (12).…”

Section: Deposition Of Airborne Plga Particles On the Co-culture Withmentioning

confidence: 99%

Autologous Co-culture of Primary Human Alveolar Macrophages and Epithelial Cells for Investigating Aerosol Medicines. Part I: Model Characterisation

Hittinger

Janke

Huwer³

et al. 2016

Altern Lab Anim

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CitationAutologous co-culture of primary human alveolar macrophages and epithelial cells for investigating aerosol medicines. Part I: model characterisation. 2016, 44 (4):337-347 Altern Lab Anim Journal Alternatives to laboratory animals : ATLA Download date 10/07/2020 16:43:37 Item License http://creativecommons.org/licenses/by-nc-sa/4.0/ Link to ItemSummary -The development of new formulations for pulmonary drug delivery is a challenge on its own. New in vitro models which address the lung are aimed at predicting and optimising the quality, efficacy and safety of inhaled drugs, to facilitate the more rapid translation of such products into the clinic.Reducing the complexity of the in vivo situation requires that such models reproducibly reflect essential physiological factors in vitro. The choice of cell types, culture conditions and the experimental set-up, can affect the outcome and the relevance of a study. In the alveolar space of the lung, epithelial cells and alveolar macrophages are the most important cell types, forming an efficient cellular barrier to aerosols. Our aim was to mimic this barrier with primary human alveolar cells. Cell densities of alveolar macrophages and epithelial cells, isolated from the same human donor, were optimised, with a focus on barrier properties. The combination of 300,000 epithelial cells/cm 2 together with 100,000 macrophages/cm 2 showed a functional barrier (transepithelial electrical resistance > 500Ω.cm 2 ). This cell model was combined with the Pharmaceutical Aerosol Deposition Device on Cell Cultures. The functionality of the in vitro system was investigated with spray-dried fluorescently labelled poly(lactic-co-glycolic) acid particles loaded with ovalbumin as a model drug.

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“…In this approach, the cylindrical pores of a membrane serve as templates for the formation of elongated NPs, which can be released upon membrane's dissolution. Despite the templateassisted deposition is specific for conducting materials, which are usually insoluble in water and thus are difficult to be employed for drug delivery applications, it represents an innovative and promising approach to prepare NEMs with elongated shape, which are expected to better deposit in deeper lung regions than spherical NPs (Kohler et al, 2011;Tscheka et al, 2015).…”

Section: Nanotechnology For Cf Treatmentmentioning

confidence: 99%

Nanomedicine Approaches for the Pulmonary Treatment of Cystic Fibrosis

Velino

Carella

Adamiano

et al. 2019

Front. Bioeng. Biotechnol.

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Cystic fibrosis (CF) is a genetic disease affecting today nearly 70,000 patients worldwide and characterized by a hypersecretion of thick mucus difficult to clear arising from the defective CFTR protein. The overproduction of the mucus secreted in the lungs, along with its altered composition and consistency, results in airway obstruction that makes the lungs susceptible to recurrent and persistent bacterial infections and endobronchial chronic inflammation, which are considered the primary cause of bronchiectasis, respiratory failure, and consequent death of patients. Despite the difficulty of treating the continuous infections caused by pathogens in CF patients, various strategies focused on the symptomatic therapy have been developed during the last few decades, showing significant positive impact on prognosis. Moreover, nowadays, the discovery of CFTR modulators as well as the development of gene therapy have provided new opportunity to treat CF. However, the lack of effective methods for delivery and especially targeted delivery of therapeutics specifically to lung tissues and cells limits the efficiency of the treatments. Nanomedicine represents an extraordinary opportunity for the improvement of current therapies and for the development of innovative treatment options for CF previously considered hard or impossible to treat. Due to the peculiar environment in which the therapies have to operate characterized by several biological barriers (pulmonary tract, mucus, epithelia, bacterial biofilm) the use of nanotechnologies to improve and enhance drug delivery or gene therapies is an extremely promising way to be pursued. The aim of this review is to revise the currently used treatments and to outline the most recent progresses about the use of nanotechnology for the management of CF.

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Macrophage uptake of cylindrical microparticles investigated with correlative microscopy

Abstract: CitationMacrophage uptake of cylindrical microparticles investigated with correlative microscopy. 2015, 95 (Pt A):151-5 Eur J Pharm Biopharm

Cited by 19 publications

References 10 publications

Visualization of Neutrophil Extracellular Traps and Fibrin Meshwork in Human Fibrinopurulent Inflammatory Lesions: III. Correlative Light and Electron Microscopic Study

Visualization of Neutrophil Extracellular Traps and Fibrin Meshwork in Human Fibrinopurulent Inflammatory Lesions: III. Correlative Light and Electron Microscopic Study

Autologous Co-culture of Primary Human Alveolar Macrophages and Epithelial Cells for Investigating Aerosol Medicines. Part I: Model Characterisation

Nanomedicine Approaches for the Pulmonary Treatment of Cystic Fibrosis

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