Macrophages directed approaches are paramount for effective cancer immunotherapies

Nadella, Vinod; Singh, Sandhya; Prakash, Hridayesh

doi:10.15761/icst.1000189

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…Among proteins that were uniquely up-regulated in M1 macrophages, seven proteins including IFIT1, IFIT2, IFIT3, CD14, CD38, CD40, and CXCL10 had been reported as M1 biomarkers or cytokines/chemokines in previous studies (33)(34)(35). Similarly, the uniquely up-regulated protein in M2 macrophage, TGM2, had also been used as a M2 biomarker (30).…”

Section: Differentially Expressed Proteins Among Three Subtypes Of Human Thp-1 Macrophagesmentioning

confidence: 99%

Comparative Proteomic Analysis of Polarized Human THP-1 and Mouse RAW264.7 Macrophages

Zou

et al. 2021

Front. Immunol.

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Macrophages can be polarized into classically activated macrophages (M1) and alternatively activated macrophages (M2) in the immune system, performing pro-inflammatory and anti-inflammatory functions, respectively. Human THP-1 and mouse RAW264.7 cell line models have been widely used in various macrophage-associated studies, while the similarities and differences in protein expression profiles between the two macrophage models are still largely unclear. In this study, the protein expression profiles of M1 and M2 phenotypes from both THP-1 and RAW264.7 macrophages were systematically investigated using mass spectrometry-based proteomics. By quantitatively analyzing more than 5,000 proteins among different types of macrophages (M0, M1 and M2) from both cell lines, we identified a list of proteins that were uniquely up-regulated in each macrophage type and further confirmed 43 proteins that were commonly up-regulated in M1 macrophages of both cell lines. These results revealed considerable divergences of each polarization type between THP-1 and RAW264.7 macrophages. Moreover, the mRNA and protein expression of CMPK2, RSAD2, DDX58, and DHX58 were strongly up-regulated in M1 macrophages for both macrophage models. These data can serve as important resources for further studies of macrophage-associated diseases in experimental pathology using human and mouse cell line models.

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Section: Differentially Expressed Proteins Among Three Subtypes Of Human Thp-1 Macrophagesmentioning

confidence: 99%

Comparative Proteomic Analysis of Polarized Human THP-1 and Mouse RAW264.7 Macrophages

Zou

et al. 2021

Front. Immunol.

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“…Due to this characteristic, these cells are now known as double-edge component of immunity as well. Many studies have demonstrated that these cells can enhance the progression as well as control many infectious and tumor [1] diseases. Both peripheral and tissue macrophages together constitute the reticuloendothelium system which plays a major role in both sensing microbial antigens and their subsequent eradication [2].…”

Section: Introductionmentioning

confidence: 99%

Macrophage Polarization Is Decisive for Chronic Bacterial Infection-Induced Carcinogenesis

Wasson¹,

Kapoor²,

Garg³

et al. 2020

Macrophage Activation - Biology and Disease

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Macrophages are the special cells of the immune system and play both immunological and physiological role. One of the peculiar characteristics of macrophages is that they are double-edged and highly plastic component of immune system. Due to this characteristic, they are responsible for both progressions as well control of a variety of inflammatory, infectious and metabolic diseases and cancer. These are found in the body in three major phenotypes, which are known as M0 (also known as naïve); M1 (classically activated macrophages); and/or M2 (alternatively activated macrophages) at normal physiological conditions. We have been exploring macrophages in context of bacterial infection and previously demonstrated that M2 polarization of M1 effector alveolar macrophages during chronic/persistent Chlamydia pneumonia, Mycobacterium tuberculosis and Helicobacter pylori pathogens are decisive for the infection induced cancer development in host. Since chronic infection with these pathogens has been associated with adenocarcinoma, therefore, we feel that disruption of macrophage plasticity plays crucial role in the host for the development of cancer. On the basis of this, we propose that in such pathological conditions, management of M1/M2 imbalance is paramount for minimizing the risk of developing cancer by chronic and persistent infection.

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Macrophages directed approaches are paramount for effective cancer immunotherapies

Cited by 2 publications

References 25 publications

Comparative Proteomic Analysis of Polarized Human THP-1 and Mouse RAW264.7 Macrophages

Comparative Proteomic Analysis of Polarized Human THP-1 and Mouse RAW264.7 Macrophages

Macrophage Polarization Is Decisive for Chronic Bacterial Infection-Induced Carcinogenesis

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