Macrophages in human breast disease: a quantitative immunohistochemical study

Kelly, P. M.; Davison, R. S.; Bliss, Eleanor A.; McGee, J O

doi:10.1038/bjc.1988.36

Cited by 154 publications

(108 citation statements)

References 22 publications

Supporting

Mentioning

105

Contrasting

Unclassified

Order By: Relevance

“…By contrast, the release of IL-4 was only detected in blood samples from 50% Effect of medium conditioned by breast cancer lines on the secretory activity of NK cells For these experiments, NK cell enriched populations of PBMC were used (see above). Figure 3 illustrates (Kelly et al, 1988).…”

Section: Resultsmentioning

confidence: 99%

Human tumour-associated NK cells secrete increased amounts of interferon-γ and interleukin-4

Lorenzen

Lewis²,

McCracken³

et al. 1991

Br J Cancer

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Human tumour-associated NK cells secrete increased amounts of interferon-γ and interleukin-4

Lorenzen

Lewis²,

McCracken³

et al. 1991

Br J Cancer

Self Cite

View full text Add to dashboard Cite

“…2,3 However, in most malignant tumors the most prominent component of this leukocytic infiltrate are macrophages (often called tumor-associated macrophages or ''TAMs''), which in some instances, comprise up to 70% of the cell tumor mass. 4 These cells are an essential cellular component of the innate immune system and are derived from myeloid progenitor cells in the bone marrow. Throughout the lifespan of an individual these stem cells develop into promonocytes and are released into the circulation, where they then differentiate into monocytes.…”

Section: Monocyte Recruitment Into Tumorsmentioning

confidence: 99%

Macrophage migration and gene expression in response to tumor hypoxia

Murdoch

Lewis

2005

Intl Journal of Cancer

184

143

View full text Add to dashboard Cite

Monocytes are recruited into tumors from the circulation along defined chemotactic gradients and they then differentiate into tumor-associated macrophages (TAMs). Recent evidence has shown that large numbers of TAMs are attracted to and retained in avascular and necrotic areas, where they are exposed to tumor hypoxia. At these sites, TAMs appear to undergo marked phenotypic changes with activation of hypoxia-inducible transcription factors, dramatically upregulating the expression of a large number of genes encoding mitogenic, proangiogenic and prometastatic cytokines and enzymes. As a consequence, high TAMs density has been correlated with increased tumor growth and angiogenesis in various tumor types. Since hypoxia is a hallmark feature of malignant tumors and hypoxic tumor cells are relatively resistant to radio-and chemotherapy, these areas have become a target for novel forms of anticancer therapy. These include hypoxiatargeted gene therapy in which macrophages are armed with therapeutic genes that are activated by hypoxia-responsive promoter elements. This restricts transgene expression to hypoxic areas, where the gene product is then released and acts on neighboring hypoxic tumor cells or proliferating blood vessels. In this way, the responses of macrophages to tumor hypoxia can be exploited to deliver potent antitumor agents to these poorly vascularized, and thus largely inaccessible, areas of tumors. ' 2005 Wiley-Liss, Inc.

show abstract

“…Tumour associated macrophages (TAMs) are widespread in human breast carcinomas (Kelly et al, 1988;Volodko et al, 1998;Lin et al, 2001;Bingle et al, 2002a) and it has been suggested that they may play an important role in the regulation of tumour angiogenesis. In vitro studies have shown that TAMs secrete growth factors that are mitogenic for both tumour cells and endothelial cells, in addition to stimulating the activity of a number of proteolytic enzymes in breast cancer (O'Sullivan et al, 1993;Pyke et al, 1993;Lewis et al, 1995;Lewis et al, 2000).…”

mentioning

confidence: 99%

Macrophages promote angiogenesis in human breast tumour spheroids in vivo

et al. 2005

View full text Add to dashboard Cite

An in vivo model has been established to study the role of macrophages in the initiation of angiogenesis by human breast tumour spheroids in vivo. The extent of the angiogenic response induced by T47D spheroids implanted into the dorsal skinfold chamber in nude mice was measured in vivo and compared to that induced by spheroids infiltrated with human macrophages prior to implantation. Our results indicate that the presence of macrophages in spheroids resulted in at least a three-fold upregulation in the release of vascular endothelial growth factor (VEGF) in vitro when compared with spheroids composed only of tumour cells. The angiogenic response measured around the spheroids, 3 days after in vivo implantation, was significantly greater in the spheroids infiltrated with macrophages. The number of vessels increased (macrophages vs no macrophages 3471.9 vs 2672.5, Po0.01), were shorter in length (macrophages vs no macrophages 11674.92 vs 13676.52, Po0.008) with an increased number of junctions (macrophages vs no macrophages 1470.93 vs 1171.25, Po0.025) all parameters indicative of new vessel formation. This is the first study to demonstrate a role for macrophages in the initiation of tumour angiogenesis in vivo.

show abstract

Macrophages in human breast disease: a quantitative immunohistochemical study

Cited by 154 publications

References 22 publications

Human tumour-associated NK cells secrete increased amounts of interferon-γ and interleukin-4

Human tumour-associated NK cells secrete increased amounts of interferon-γ and interleukin-4

Macrophage migration and gene expression in response to tumor hypoxia

Macrophages promote angiogenesis in human breast tumour spheroids in vivo

Contact Info

Product

Resources

About