Macrophages influence gap junctional intercellular communication between smooth muscle cells in a co-culture model

Mensink, A.; Mul, Frederik P. J.; Wijk, Thea van der; Brouwer, Abraham; Koeman, J.H.

doi:10.1016/s1382-6689(98)00002-7

Cited by 2 publications

(2 citation statements)

References 44 publications

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“…This does not resemble adequately the in vivo situation when a time-resolved arrival of cells on the implant surface occurs and a specific spatial and time-dependent release and exchange of signaling molecules occurs. Other experimental settings used membranes to separate the different cell types spatially that no direct contact can occur even at later stages to study the role of released signaling molecules involved in the inflammatory reaction [22].…”

Section: Introductionmentioning

confidence: 99%

A macrophage/fibroblast co-culture system using a cell migration chamber to study inflammatory effects of biomaterials

2015

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Section: Introductionmentioning

confidence: 99%

A macrophage/fibroblast co-culture system using a cell migration chamber to study inflammatory effects of biomaterials

2015

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“…In turn, ROS mediate the expression of MMP2, the cleavage of caspase‐3, and partially of Cox‐2 expression in MCs. The interaction between macrophages and SMCs has also been studied in the physiopathology of atherosclerosis (52–55), an inflammatory disease (56). Indeed, macrophages have a deleterious effect in the arterial wall by inducing SMC apoptosis, as well as digestion of the extracellular matrix, leading to the rupture of atherosclerotic plaques (56).…”

Section: Discussionmentioning

confidence: 99%

Glutathione prevents preterm parturition and fetal death by targeting macrophage‐induced reactive oxygen species production in the myometrium

et al. 2015

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Preterm birth is an inflammatory process resulting from the massive infiltration of innate immune cells and the production of proinflammatory cytokines in the myometrium. However, proinflammatory cytokines, which induce labor in vivo, fail to induce labor-associated features in human myometrial cells (MCs). We thus aimed to investigate if reactive oxygen species (ROS) production could be the missing step between immune cell activation and MC response. Indeed, we found that ROS production is increased in the human preterm laboring myometrium (27% ROS producing cells, respectively, versus 2% in nonlaboring controls), with 90% ROS production in macrophages. Using LPS-stimulated myometrial samples and cell coculture experiments, we demonstrated that ROS production is required for labor onset. Furthermore, we showed that ROS are required first in the NADPH oxidase (NADPHox)-2/NF-kB-dependent macrophage response to inflammatory stimuli but, more importantly, to trigger macrophage-induced MCs transactivation. Remarkably, in a murine model of LPS-induced preterm labor (inducing delivery within 17 hours, with no pup survival), cotreatment with glutathione delayed labor onset up to 94 hours and prevented in utero fetal distress, allowing 46% pups to survive. These results suggest that targeting ROS production with the macrophage-permeable antioxidant glutathione could constitute a promising strategy to prevent preterm birth.-Hadi, T., Bardou, M., Mace, G., Sicard, P., Wendremaire, M., Barrichon, M., Richaud, S., Demidov, O., Sagot, P., Garrido, C., Lirussi, F. Glutathione prevents preterm parturition and fetal death by targeting macrophage-induced reactive oxygen species production in the myometrium. FASEB J. 29, 2653-2666 (2015). www.fasebj.org

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Macrophages influence gap junctional intercellular communication between smooth muscle cells in a co-culture model

Cited by 2 publications

References 44 publications

A macrophage/fibroblast co-culture system using a cell migration chamber to study inflammatory effects of biomaterials

A macrophage/fibroblast co-culture system using a cell migration chamber to study inflammatory effects of biomaterials

Glutathione prevents preterm parturition and fetal death by targeting macrophage‐induced reactive oxygen species production in the myometrium

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