Macroporous biohybrid cryogels for co-housing pancreatic islets with mesenchymal stromal cells

Borg, Danielle J.; Welzel, Petra B.; Grimmer, Milauscha; Friedrichs, Jens; Weigelt, Marc; Wilhelm, Carmen; Prewitz, Marina; Stißel, Aline; Hommel, Angela; Kurth, Thomas; Freudenberg, Uwe; Bonifacio, Ezio; Werner, Carsten

doi:10.1016/j.actbio.2016.08.007

Cited by 42 publications

(39 citation statements)

References 52 publications

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“…Due to their interconnected, macroporous 3D structure, cryogels have been widely used in bioseparation‐related applications [1–10], tissue engineering [10–14], and other relevant bioengineering and sensor applications [15–17]. In particular, monolithic cryogels have been used as a new generation of chromatographic matrices for the separation of cells (mammalian, bacterial, and yeast), proteins, viruses, and plasmids [18–22].…”

Section: Introductionmentioning

confidence: 99%

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Sun

Feng

Zhong

et al. 2020

J of Separation Science

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A novel, facile, and robust strategy was proposed to increase the pore size and mechanical strength of cryogels. By mixing the monomers of acrylamide and 2‐hydroxyethyl methacrylate as the precursor, a monolithic copolymer cryogel with large interconnected pores and thick pore walls was prepared. Hydrogen bonding between the two monomers contributed to the entanglement and aggregation of the copolymers, thickening the pore walls and resulting in larger pore sizes. Analysis via mercury porosimetry demonstrated that the interconnected pore diameter of the copolymer cryogel ranged from 10‐350 µm, which was far larger than that of the cryogels from one monomer (10‐50 µm). Additionally, the thicker pore walls of the copolymer cryogel improved its mechanical strength. Affinity cryogels were prepared through covalent immobilization using Tris(hydroxymethyl)aminomethane as a coupling agent, and the affinity binding of lysozymes on Tris‐cryogel was evaluated by the Langmuir isothermal adsorption with the maximum adsorption capacity of 360 mg/g. Compared with that of the Tris‐cryogels produced from one monomer, the copolymer Tris‐cryogel exhibited higher adsorption capacity and lysozyme purity, when the chicken egg white solution flowed solely driven by gravity. This work provides a new avenue for designing and developing supermacroporous cryogels for bioseparation.

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Section: Introductionmentioning

confidence: 99%

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Sun

Feng

Zhong

et al. 2020

J of Separation Science

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“…We have utilized PEG-HEP matrices to determine the biochemical and biophysical cues necessary for the survival and morphogenesis of several cell types, including endothelial cells [16, 20, 22, 26], neurons [16], and pancreatic islets [28]. Therefore, we hypothesized that PEG-HEP hydrogels could be an exemplary matrix to study the interplay between the microenvironment and complex mammary epithelium multi-cellular morphogenesis in a 3D in vivo -like context.…”

Section: Resultsmentioning

confidence: 99%

Modular GAG-matrices to promote mammary epithelial morphogenesis in vitro

et al. 2017

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Matrix systems used to study complex three-dimensional (3D) cellular processes like mammary epithelial tissue morphogenesis and tumorigenesis ex vivo often require ill-defined biological components, which lead to poor reproducibility and a lack of control over physical parameters. In this study, a well-defined, tunable synthetic biohybrid hydrogel composed of the glycosaminoglycan heparin, star-shaped poly(ethylene glycol) (starPEG), and matrix metalloproteinase- (MMP-) cleavable crosslinkers was applied to dissect the biophysical and biochemical signals promoting human mammary epithelial cell (MEC) morphogenesis. We show that compliant starPEG-heparin matrices promote the development of polarized MEC acini. Both the presence of heparin and MMP-cleavable crosslinks are essential in facilitating MEC morphogenesis without supplementation of exogenous adhesion ligands. In this system, MECs secrete and organize laminin in basement membrane-like assemblies to promote integrin signaling and drive acinar development. Therefore, starPEG-heparin hydrogels provide a versatile platform to study mammary epithelial tissue morphogenesis in a chemically defined and precisely tunable 3D in vitro microenvironment. The system allows investigation of biophysical and biochemical aspects of mammary gland biology and potentially a variety of other organoid culture studies.

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“…Cryogels have played significant role in bioreactor systems, a noteworthy focus of tissue engineering, for the expansion and modification of various cells, as well as the production of signaling molecules (Hixon et al, 2017;Obregón et al, 2016;Martin et al, 2004). Specifically, cryogels have shown promise as vehicles for both the storage and transportation of cells (Vrana et al, 2012;Katsen-Globa et al, 2014;Borg et al, 2016). Beyond storage, cryogels also have the potential to be used as bioreactors, with the surface area-to-volume ratio proving ideal for culturing large quantities of cells Çimen & Denizli, 2012;Eichhorn et al, 2013).…”

Section: Bioreactormentioning

confidence: 99%

Three‐dimensional cryogels for biomedical applications

Razavi

Qiao

Thakor

2019

J Biomedical Materials Res

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Cryogels are a subset of hydrogels synthesized under sub‐zero temperatures: initially solvents undergo active freezing, which causes crystal formation, which is then followed by active melting to create interconnected supermacropores. Cryogels possess several attributes suited for their use as bioscaffolds, including physical resilience, bio‐adaptability, and a macroporous architecture. Furthermore, their structure facilitates cellular migration, tissue‐ingrowth, and diffusion of solutes, including nano‐ and micro‐particle trafficking, into its supermacropores. Currently, subsets of cryogels made from both natural biopolymers such as gelatin, collagen, laminin, chitosan, silk fibroin, and agarose and/or synthetic biopolymers such as hydroxyethyl methacrylate, poly‐vinyl alcohol, and poly(ethylene glycol) have been employed as 3D bioscaffolds. These cryogels have been used for different applications such as cartilage, bone, muscle, nerve, cardiovascular, and lung regeneration. Cryogels have also been used in wound healing, stem cell therapy, and diabetes cellular therapy. In this review, we summarize the synthesis protocol and properties of cryogels, evaluation techniques as well as current in vitro and in vivo cryogel applications. A discussion of the potential benefit of cryogels for future research and their application are also presented.

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Macroporous biohybrid cryogels for co-housing pancreatic islets with mesenchymal stromal cells

Cited by 42 publications

References 52 publications

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Modular GAG-matrices to promote mammary epithelial morphogenesis in vitro

Three‐dimensional cryogels for biomedical applications

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