Macroporous, protein-containing films cast from water-in-oil emulsions featuring a block-copolymer

Abstract

Patterned biohybrid films show great potential for application in biosensing, bioconversion, and biochips. Their preparation generally involves a multistep procedure including patterned film fabrication and biomolecule immobilization. Here, we report a one-step approach to well-ordered protein-containing films by the self-assembly of a reverse emulsion (water-in-oil), which was stabilized by an amphiphilic block copolymer polystyrene-block-poly(2-hydroxyethyl methacrylate) (PS-b-PHEMA). We investigated the effects of the solvent, polymer concentration, PHEMA chain length, and substrate on the surface morphologies. The merging of the as-formed emulsion droplets, the formation and refilling of the gaps between the pores, and the hierarchical fine structures surrounding the pores were observed. Dynamic evolution results revealed the formation process of the films. Onestep preparation of protein-containing films was achieved by introducing proteins into the reverse emulsion droplets. The protein pattern was further enhanced by the reduced-pressure method, which largely confined the capillary flow. This method is versatile to various water-soluble functional substances and opens a straightforward route for functional honeycomb-patterned porous films.

Porous Polymeric Films from Microbubbles Generated Using a T-Junction Microfluidic Device

“…However, the resulting scaffolds contained a wide pore size distribution. The ability to incorporate biomolecules (such as enzymes) into the porous films produced by multi-step methods, such as breath figure (8), can be greatly limited, since organic solvents (such as dichloromethane and carbon disulfide) are involved in the preparation process (9), which often damage the biomolecules. On the other hand, the more simplified (one-step) methods, such as the conventional casting of films (3) produce porous films with non-controlled pore sizes (10).…”

Fabrication of honeycomb films from highly functional dendritic structures: electrostatic force driven immobilization of biomolecules

“…In contrast, others incorporated the biomolecule into the polymeric solution and prepared the films by casting. 25 The latter led, in the reported procedures, to a homogeneous distribution of protein over the entire material. However, in some cases, a particular biomolecule distribution as well as adjusting the biomolecule density present at the surface are key aspects that require to be controlled.…”

Construction of ordered porous polymer film and functionality of pore structure via microemulsion template method

“…寻着乳 液多孔材料的思路, 本课题组 [82] [82] Figure 4 SEM images viewed from top surface (a) and cross section (b) of EuSiW 11 /PMMA hybrid film [82] 形成的图案结构并不受影响, 而功能化物质则在水 滴挥发后保留在孔洞内, 将在下文讨论. [82] Figure 7 (Color online) (a) SEM image and EDX analysis of Eu-SiW 11 -incorporated porous PMMA film on glass substrate for element distribution of C (b), Si (c), and W (d) [82] Zhang等人 [83] 片, 其他均为光学显微镜结果 [83] Figure 8 (Color online) Micrographs of honeycomb films prepared from PS-b-PHEMA 60 reverse emulsion in CS 2 (a, b) and CH 2 Cl 2 (c, d) [83] [82] Figure 9 (Color online) CLSM images of EuW 10 patterned PMMA film before (a) and after encountering the immersion in hemoglobin-Cy3 (b) and BSA-FITC aqueous solutions (c) with the same concentration for 30 min [82] 图 10 孔膜孔洞内表面修饰、负载和释放胰岛素聚集体的化学过程示意图 [86]…”