This research presents for the first time a comprehensive study of charcoal directly related to the multiple burials interred in Tomb 2 of El Caño (Coclé province, Panama). This funerary context, which dates to between AD 880 to AD 1200, contained three different burial levels accompanied by substantial ceramic offerings and rich mortuary assemblages. The challenge of taxonomically identifying charcoal from tropical areas was addressed by combining standard procedures in tandem with chemical analysis (Py-GC-MS) of archaeological charcoal and fresh wood from the reference collection. Nine charcoal types were identified at the genus level: AVICENNIA SPP., RHIZOPHORA SPP., HANDRANTHUS/TABEBUIA, CF. COPAIFERA SPP., INGA SPP., GUETTARDA SPP., ROUPALA SPP., ALLOPHYLUS SPP. and CF. RYANIA SPP. Charcoal of CF. COPAIFERA SPP., ROUPALA SPP., GUETTARDA SPP., and probably HANDROANTHUS/TABEBUIA might be related to their use as firewood for producing smoke. The presence of Copaifera and Roupala indicates the selection of odorous woods for burning in the sahumerios. Diverse habitats, such as coastal mangroves, riverine and dry forests, were exploited to obtain wood, highlighting the complex management of wild plant resources developed by the hierarchical societies of the Isthmo-Colombian area.