MADS-box genes active in developing pollen cones of Norway spruce (Picea abies) are homologous to the B-class floral homeotic genes in angiosperms

Sundström, Jens F.; Carlsbecker, Annelie; Svensson, Mats; Svenson, Marie; Johanson, Urban; Theißen, Günter; Engström, Peter

doi:10.1002/(sici)1520-6408(1999)25:3<253::aid-dvg8>3.0.co;2-p

Cited by 109 publications

(111 citation statements)

References 47 publications

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“…Probes directed towards the AP3 homologue in P. abies, DAL13, produced signals in the microsporophylls of P. abies male cones both before and after apical meristem termination ( Fig. 2C and D), as expected from previous studies 7,14 Figure 3. DAL13 expression in male cones from P. abies.…”

supporting

confidence: 86%

“…The genetic mechanism that regulates reproductive development in gymnosperms and angiosperms by specifying male and female organ identity appears to be evolutionary conserved [7][8][9] despite that the two seed plant lineages separated 285 million years ago 10 . In A. thaliana the floral homeotic genes APETALA3 (AP3…”

Section: Resultsmentioning

confidence: 99%

“…In gymnosperms, which lack flowers and have their reproductive organs arranged in separate male and female cones (Fig 1A-C), genes homologous to AP3 and PI are specifically expressed in the pollen bearing organs of the male cone, the microsporophylls 7,14 . Hence, a similar set of homologous genes in both angiosperms and gymnosperms defines the pollen-bearing organs.…”

mentioning

confidence: 99%

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Non-radioactive <em>in situ</em> Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Karlgren

Carlsson

Gyllenstrand

et al. 2009

JoVE

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The high-throughput expression analysis technologies available today give scientists an overflow of expression profiles but their resolution in terms of tissue specific expression is limited because of problems in dissecting individual tissues. Expression data needs to be confirmed and complemented with expression patterns using e.g. in situ hybridization, a technique used to localize cell specific mRNA expression. The in situ hybridization method is laborious, time-consuming and often requires extensive optimization depending on species and tissue. In situ experiments are relatively more difficult to perform in woody species such as the conifer Norway spruce (Picea abies). Here we present a modified DIG in situ hybridization protocol, which is fast and applicable on a wide range of plant species including P. abies. With just a few adjustments, including altered RNase treatment and proteinase K concentration, we could use the protocol to study tissue specific expression of homologous genes in male reproductive organs of one gymnosperm and two angiosperm species; P. abies, Arabidopsis thaliana and Brassica napus. The protocol worked equally well for the species and genes studied. AtAP3 and BnAP3 were observed in second and third whorl floral organs in A. thaliana and B. napus and DAL13 in microsporophylls of male cones from P. abies. For P. abies the proteinase K concentration, used to permeablize the tissues, had to be increased to 3 g/ml instead of 1 g/ml, possibly due to more compact tissues and higher levels of phenolics and polysaccharides. For all species the RNase treatment was removed due to reduced signal strength without a corresponding increase in specificity. By comparing tissue specific expression patterns of homologous genes from both flowering plants and a coniferous tree we demonstrate that the DIG in situ protocol presented here, with only minute adjustments, can be applied to a wide range of plant species. Hence, the protocol avoids both extensive species specific optimization and the laborious use of radioactively labeled probes in favor of DIG labeled probes. We have chosen to illustrate the technically demanding steps of the protocol in our film.

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supporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Non-radioactive <em>in situ</em> Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Karlgren

Carlsson

Gyllenstrand

et al. 2009

JoVE

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“…genes were involved in specification of male reproductive development in the nonflowering ancestor of the angiosperms, similar to the role that homologs of these genes are thought to play in extant gymnosperms (49)(50)(51)(52). Within the angiosperms, this role in specification of male reproductive structures (stamens) has been retained, but different clades of plant species have deployed these genes independently [i.e., grasses and core eudicots (53)] to specify sterile organs within the flower and, in at least some instances, have evolved new protein motifs to support these new functions.…”

Section: Discussionmentioning

confidence: 68%

Functional divergence within the APETALA3/PISTILLATA floral homeotic gene lineages

Lamb

Irish

2003

Proc. Natl. Acad. Sci. U.S.A.

192

215

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Changes in homeotic gene expression patterns or in the functions of the encoded proteins are thought to play a prominent role in the evolution of new morphologies. The floral homeotic APETALA3 (AP3) and PISTILLATA (PI) genes encode MADS domain-containing transcription factors required to specify petal and stamen identities in Arabidopsis. We have previously shown that perianth expression of AP3 and PI homologs varies in different groups of angiosperms with diverse floral structures, suggesting that changes in expression may contribute to changing morphology. We have investigated the possibility that changes in the functions of the encoded gene products may also have played a role in the evolution of different floral morphologies. AP3 and PI are members of paralogous gene lineages and share extensive similarity along the length of the protein products. Genes within these lineages encode products with characteristic C-terminal motifs that we show are critical for functional specificity. In particular, the C terminus of AP3 is sufficient to confer AP3 functionality on the heterologous PI protein. Furthermore, we have shown that the evolution of the divergent AP3 C-terminal domain in the core eudicots is correlated with the acquisition of a role in specifying perianth structures. These results suggest that divergence in these sequence motifs has contributed to the evolution of distinct functions for these floral homeotic gene products.

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“…Algunos autores han observado que los genes del grupo AG tienen una estructura intrón/exón particular (Tandre et al, 1998;Rutledge et al, 1998;Jager et al, 2003). Incluso, las mismas posiciones de los intrones se han descrito en genes MADS-box no pertenecientes a este grupo (Sundström et al, 1999), lo que sugiere que la estructura intrón/exón ha sido un rasgo muy conservado durante la evolución de los genes MIKC. (Jager et al, 2003).…”

Section: Mtagaunclassified

Aislamiento y caracterización de genes MADS-box en Medicago truncatula: duplicaciones génicas y subfuncionalización en el linaje euAGAMOUS

Serwatowska¹

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AGRADECIMIENTOSMi más sincero agradecimiento a todas las personas que de una manera u otra, de cerca o de lejos, me han apoyado durante los últimos nueve años.En primer lugar, quisiera agradecer a mis directores de Tesis, Dr. Luis Antonio Cañas Clemente y Dr. José Pío Beltrán Porter, por la oportunidad que me brindaron de realizar este trabajo y por la confianza depositada en mí.A José María Bellés, por tutelar este trabajo y apoyarme cuando lo necesité.A Kirankumar S. Mysore y Jiangqi Wen (Samuel Roberts Foundation, Oklahoma, USA)por facilitarnos las semillas de la línea NF4908 con inserción del retrotransposón Tnt1.A los Drs. Elisabeth I. Johansen, Ole S. Lund y Gabriela D. Constantin por dejarme ser parte de su laboratorio durante mi estancia en Frederiksberg, Dinamarca. Por formarme y ayudarme en el desarrollo de los experimentos VIGS presentados en esta Tesis.Quiero hacer constar mi agradecimiento a todo el personal del IBMCP (Administración y Servicios), cuya labor ha sido imprescindible para llevar a cabo el presente trabajo. En especial a Santiago y a Rafa, por su trato siempre afectuoso y amable.A Ana y a Majo por estar al pendiente tanto de mis Arabidopsis como de mí.A Marisol y a MD, por el aporte cultural en los almuerzos.Gracias a todos mis compañeros de laboratorio, a los de siempre y a los recién llegados, por su cariño y por soportarme cada día en las buenas y en las malas.A Edelín, Maricruz, Bego, Auri y Tere, sin cuyo trabajo el mío no habría sido posible.A Concha por sus ideas claras y por hacerme ver que mi trabajo es bueno. Gracias de corazón.Además, quiero agradecer a muchas personas que han llegado a formar parte de mi vida en estos últimos años y sin cuya amistad y apoyo no me habría sentido "en casa" estando del otro lado del mundo.Gracias a mis amigos, a los de aquí y a los de allá, por estar.A Mónica, por su amistad, su compañía y por ser tan buena. Espero que sigamos viviendo juntas muchos más momentos inolvidables.A Marina, por ser tan buena amiga y por los estupendos ratos pasados.A Lina, por la empatía en los malos tiempos que finalmente no fueron tan malos.A José Manuel y a Susana, por estar ahí.A Rosalía, Indalecio, Inés, Maricha, Jose, Jose Luis e Inesita por cuidarme y haberme acogido como una más de la familia.A mis padres por mostrarme el significado de la constancia y el trabajo bien hecho y por darme la oportunidad de llegar hasta aquí.A Roberto, porque sin ti no habría llegado.Y a Rysio. En definitiva, esto es para ti.El presente trabajo ha sido financiado por una beca-contrato Predoctoral para el Desarrollo de Tesis Doctoral en Líneas de Investigación con Interés para el SectorIndustrial, así como por la Ayuda Complementaria para la Realización de Estancias Breves en Centros de I+D y Empresas en España y en el Extranjero, concedidas por el CSIC.Ukochanym Rodzicom A Roberto R R E E S S Ú Ú M M E E N N E E S S Resúmenes Aislamiento y caracterización de genes MADS-box en Medicago truncatula: duplicaciones génicas y subfuncionalización en el linaje euAGAMOUSLas angiospermas p...

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MADS-box genes active in developing pollen cones of Norway spruce (Picea abies) are homologous to the B-class floral homeotic genes in angiosperms

Cited by 109 publications

References 47 publications

Non-radioactive <em>in situ</em> Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Non-radioactive <em>in situ</em> Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Functional divergence within the APETALA3/PISTILLATA floral homeotic gene lineages

Aislamiento y caracterización de genes MADS-box en Medicago truncatula: duplicaciones génicas y subfuncionalización en el linaje euAGAMOUS

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