MAEL gene contributes to bovine testicular development through the m5C-mediated splicing

Molecular quantitative trait loci in reproductive tissues impact male fertility in cattle

“…. Comparing our findings with similar-sized cohorts sampled at other developmental stages and the analysis of single-cell transcriptomes will reveal spatiotemporal transcriptomic changes in male reproductive tissue, such as those occurring during testicular development and puberty [42][43][44] .…”

Toward understanding and utilizing crop heterosis in the age of biotechnology

“… 11 How substitution of half the parental genome in the hybrid generates different degrees of leaf size heterosis was found to be attributable to a distinctly increased number of photosynthetic cells due to the expression upregulation degree of cell cycle genes, where the expression change in the F 1 hybrid with high-level heterosis was found to be optimized by superior heterozygous eQTL accumulation. 11 In maize, through a design of multiple linked F 1 populations containing 42,840 F 1 hybrids and genome-wide association study, an instance of epistasis was revealed where one recessive, deleterious maternal allele, Brachytic2 , repressed the favorable Ubiquitin3 locus in the maternal lines, while the paternal allele alleviated this repression and thus recovered plant height and ear weight in hybrids. 10 The genomic architectures of heterosis in rice, maize, wheat, and other crops were also characterized using the population-based method, 6 , 7 , 12 , 13 , 26 , 27 , 44 which uncovered many novel loci functioning in heterosis via dominance, overdominance, and epistasis.…”

“… 4 , 5 , 6 , 7 , 8 In particular, population genetic studies on heterosis performance variation provided new insights into the genetic basis of heterosis and the principles for parental selection in hybrid breeding. 9 , 10 Recent studies integrating new tools such as genome-wide and transcriptome-wide association studies, 10 , 11 , 12 , 13 , 14 long-read sequencing, 15 , 16 and three-dimensional (3D) genome scanning 17 have uncovered many genetic loci for genomics-based hybrid breeding. Strategies like gene regulatory network analysis 8 and single-cell transcriptome profiling 18 , 19 have dug out the key gene expression changes behind heterosis.…”

“…Studies using a unique design of half-sibling F 1 hybrid populations uncovered the underlying molecular regulatory basis and identified key genetic components. 10 , 11 In Arabidopsis , new information regarding heterosis regulation was obtained by association studies on the transcriptomic landscape of a half-sibling hybrid population using transcriptome-wide association studies and expression quantitative trait loci (eQTL). 11 How substitution of half the parental genome in the hybrid generates different degrees of leaf size heterosis was found to be attributable to a distinctly increased number of photosynthetic cells due to the expression upregulation degree of cell cycle genes, where the expression change in the F 1 hybrid with high-level heterosis was found to be optimized by superior heterozygous eQTL accumulation.…”

“… 10 , 11 In Arabidopsis , new information regarding heterosis regulation was obtained by association studies on the transcriptomic landscape of a half-sibling hybrid population using transcriptome-wide association studies and expression quantitative trait loci (eQTL). 11 How substitution of half the parental genome in the hybrid generates different degrees of leaf size heterosis was found to be attributable to a distinctly increased number of photosynthetic cells due to the expression upregulation degree of cell cycle genes, where the expression change in the F 1 hybrid with high-level heterosis was found to be optimized by superior heterozygous eQTL accumulation. 11 In maize, through a design of multiple linked F 1 populations containing 42,840 F 1 hybrids and genome-wide association study, an instance of epistasis was revealed where one recessive, deleterious maternal allele, Brachytic2 , repressed the favorable Ubiquitin3 locus in the maternal lines, while the paternal allele alleviated this repression and thus recovered plant height and ear weight in hybrids.…”