MafB, a target of microRNA-155, regulates dendritic cell maturation

Abstract

MafB is a member of bZip transcription factors that share similar basic region/leucine zipper DNA binding motifs and N-terminal activation domains. It is well known that MafB is highly expressed in macrophages and promotes differentiation of myeloid progenitors into macrophage. However, little is known about its function in dendritic cells. Here, we report that MafB, as a target of miR-155, which had been reported to be required for dendritic cell maturation and function, regulated dendritic cell maturation. MafB and miR-155were reversely correlated during DC maturation induced by LPS and forced expression of miR-155 reduced MafB expression. The luciferase reporter assay showed that MafB 3’UTR was directly targeted bymiR-155. In addition, knockdown of MafB promoted the phenotypic maturation of DC2.4 cells. Forced expression of MafB could significantly attenuate the phenotypic maturation of DC2.4 cells caused by overexpression of miR-155. Overall, our data demonstrates that MafB, inhibited by miR-155, was a negative regulator of DC maturation.

Single-cell immunophenotyping of the fetal immune response to maternal SARS-CoV-2 infection in late gestation

“… 42 Evidence of impaired cDC maturation was suggested by upregulation of ID1 , which antagonizes dendritic cell differentiation and antitumor immunity in mice, 43 as well as increased MAFB transcription, which suppresses cDC maturation. 44 cDCs from cases also demonstrated decreased expression of FOSB and many MHC II genes. 45 pDCs in cases also showed markers of immune activation, including upregulation of RELB , which promotes DC activation through RelB-p50 dimer, 46 upregulation of MHC class I and class II genes, and unfolded protein response activation, as shown by increased transcription of XBP1 47 (Supplemental Fig.…”

Expression level of fibroblast growth factor 5 (FGF5) in the peripheral blood of primary hypertension and its clinical significance

“…In recent years, with the continuous development of the whole gene sequencing and restriction fragment length polymorphism (RFLP) study, the genome-wide association studies (GWAS) based on single nucleotide polymorphism (SNP) chip technology provided a great progress for the study of pathogenesis of primary hypertension (Wellcome Trust Case Control Consortium, 2007, Levy et al, 2007). A large number of GWAS reported several hypertension susceptibility gene in primary hypertension patients from Europe, China, Japan and Korea, which laid the foundation for the study of the pathogenesis of hypertension (Hong et al, 2009, Ehret et al, 2008, Maruthamuthu and Kandasamy, 2016, Yang et al, 2016, Zhu et al, 2011). FGF5 is one of the fibroblast growth factors and the existing studies showed that it played an important role in the control of the animal hair growth process (Higgins et al, 2014, Kubelkova and Macela, 2015, Haitham, 2016, Chen et al, 2013).…”

Single-cell immunophenotyping of the fetal immune response to maternal SARS-CoV-2 infection in late gestation

“…2E) 38 . Evidence of impaired cDC maturation was suggested by upregulation of ID1, which antagonizes dendritic cell differentiation and antitumor immunity in mice 39 , as well as increased MAFB transcription, which suppresses cDC maturation 40 . cDCs from cases also demonstrated decreased expression of FOSB and many MHC II genes 41 .…”