Magnesium deficiency enhances oxidative stress and collagen synthesis in vivo in the aorta of rats

Shivakumar, K.; Kumar, Balawant

doi:10.1016/s1357-2725(97)00068-x

Cited by 44 publications

(23 citation statements)

References 25 publications

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“…Superoxide is known to stimulate collagen synthesis by hepatocytes and fibroblasts in vitro (9,17), and a prooxidant system also induces collagen gene expression and protein production in liver fat-storing cells (35). In addition, oxidative stress, as evidenced by increases in TBARS, stimulates collagen synthesis in the rat aorta in vivo (41). Because oxLDL stimulates reactive oxygen species production by SMCs (16), oxLDL may upregulate collagen production through a redox-sensitive pathway.…”

Section: Discussionmentioning

confidence: 99%

A comparison of oxidized LDL-induced collagen secretion by graft and aortic SMCs: role of PDGF

Absood¹,

Furutani²,

Kawamura³

et al. 2004

American Journal of Physiology-Heart and Circulatory Physiology

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Smooth muscle cells (SMCs) from prosthetic vascular grafts constitutively secrete higher levels of collagen than aortic SMCs. Lipid oxidation products accumulate in grafts, and we postulated that they stimulate SMC production of collagen. The effect of oxidized low-density lipoprotein (oxLDL) on type I collagen secretion by aortic and graft SMCs was compared. SMCs isolated from the canine thoracic aorta or Dacron thoracoabdominal grafts (n = 10) were incubated with native LDL or oxLDL (0-400 microg cholesterol/ml) for 72 h. Type I collagen in the conditioned medium was measured by ELISA. OxLDL increased collagen production by graft SMCs from 4.1 +/- 0.3 to 11.0 +/- 0.4 ng/microg DNA and by aortic SMCs from 2.3 +/- 0.1 to 3.5 +/- 0.2 ng/microg DNA. Native LDL had little effect. LY-83583, a superoxide generator, stimulated a dramatic increase in collagen secretion by graft SMCs and a smaller but significant elevation by aortic SMCs. OxLDL has been shown to increase PDGF production by graft SMCs, and PDGF can stimulate collagen production. Anti-PDGF antibody inhibited the increase in collagen production by graft SMCs that was stimulated by oxLDL, implicating PDGF as one mechanism of oxLDL-induced collagen production. Lipid oxidation products that accumulate in prosthetic vascular grafts can cause an oxidative stress that stimulates PDGF production by graft SMCs that in turn stimulates collagen production, contributing to the progression of intimal hyperplasia.

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Section: Discussionmentioning

confidence: 99%

A comparison of oxidized LDL-induced collagen secretion by graft and aortic SMCs: role of PDGF

Absood¹,

Furutani²,

Kawamura³

et al. 2004

American Journal of Physiology-Heart and Circulatory Physiology

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“…In another study, rats fed the MgD diet displayed an impaired redox capacity, marked by increased levels of thiobarbituric acid-reactive substances and oxysterols in the plasma as well as an increased susceptibility of RBC to freeradical- induced haemolysis in vitro [38]. High levels of thiobarbituric acid-reactive substances in the aorta of rats fed the Mg deficient diet correlated with a significant reduction in the activity of superoxide dismutase and catalase as well as an increase in the net fractional rates of collagen synthesis [39]. In mice, hypomagnesaemia led to a decrease in GSH concentration and lowered the activity of superoxide dismutase, glutathione reductase, and glutathione S-transferase in RBCs.…”

Section: Oxidative Stress and Magnesium Deficiencymentioning

confidence: 99%

Magnesium deficiency and oxidative stress: an update

Желтова¹,

Kharitonova²,

Iezhitsa³

et al. 2016

BioMed

167

102

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Magnesium deficiency (MgD) has been shown to impact numerous biological processes at the cellular and molecular levels. In the present review, we discuss the relationship between MgD and oxidative stress (OS). MgD is accompanied by increased levels of OS markers such as lipid, protein and DNA oxidative modification products. Additionally, a relationship was detected between MgD and a weakened antioxidant defence. Different mechanisms associated with MgD are involved in the development and maintenance of OS. These mechanisms include systemic reactions such as inflammation and endothelial dysfunction, as well as changes at the cellular level, such as mitochondrial dysfunction and excessive fatty acid production.

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“…Magnesium deficiency has been shown to produce vascular lesions in experimental animals (Rude, 1992) and intracellular calcium elevation, resulting in increased vascular tone and hypertension (Shivakvmar and Kumar, 1997). Several prospective studies have indicated that hypertension in diabetic patients takes place at a rate more than twice compared to the normal population.…”

Section: Introductionmentioning

confidence: 99%

Oral magnesium administration prevents vascular complications in STZ-diabetic rats

et al. 2005

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Magnesium deficiency enhances oxidative stress and collagen synthesis in vivo in the aorta of rats

Cited by 44 publications

References 25 publications

A comparison of oxidized LDL-induced collagen secretion by graft and aortic SMCs: role of PDGF

A comparison of oxidized LDL-induced collagen secretion by graft and aortic SMCs: role of PDGF

Magnesium deficiency and oxidative stress: an update

Oral magnesium administration prevents vascular complications in STZ-diabetic rats

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