1977
DOI: 10.1085/jgp.69.4.389
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Magnesium efflux in dialyzed squid axons.

Abstract: The efflux of Mg ++ from squid axons subject to internal solute control by dialysis is a function of ionized [Mg]l, [Nail, [ATP]I, and [Na]o. The efflux of Mg ++ from an axon with physiological concentrations of ATP, Na, and Mg inside into seawater is of the order of 2-4 pmol/cm2s but this efflux is strongly inhibited by increases in [Na~, by decreases in [ATP]t, or by decreases in [Na]o. The efflux of Mg ++ is largely independent of [Mg]i when ATP is at physiological levels, but in the absence of ATP reaches … Show more

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Cited by 33 publications
(20 citation statements)
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“…This suggests that the Ca2+/H+ antiporter has been eliminated during the solubilization-reconstitution step (only 10% of the initial protein content was recovered in these vesicles). In animal cells, it has been previously proposed that the same transporter may be responsible for both M$+ and Cai!+ transport (Mullins et al, 1977), but it appears now that Mg2+ needs its own, separate transport system (see Flatman, :!991, for a review).…”
Section: Discussionmentioning
confidence: 99%
“…This suggests that the Ca2+/H+ antiporter has been eliminated during the solubilization-reconstitution step (only 10% of the initial protein content was recovered in these vesicles). In animal cells, it has been previously proposed that the same transporter may be responsible for both M$+ and Cai!+ transport (Mullins et al, 1977), but it appears now that Mg2+ needs its own, separate transport system (see Flatman, :!991, for a review).…”
Section: Discussionmentioning
confidence: 99%
“…28 Mg, the only useful radioisotope of Mg 2+ has a very short half-life (21 h) and is currently being produced on only 1 day each year in the United States; 2.The use of squid axons is limited by their seasonal availability (3-4 months/year) and by the fact that, due to the fragility of the squid, the experiments have to be carried out at the place where they are captured (e.g., Marine Biological Laboratory, Woods Hole, MA); 3.Na ) (24, and see below). Thus, ability to carry out systematic studies of these fluxes is limited by their intrinsic low signal/noise ratio.…”
Section: Evidence Formentioning
confidence: 99%
“…The first one is technical and consists of overcoming the limitations to study ionic fluxes mediated by the Mg 2+ transporter, the second is scientific and consists of showing that, besides the electrochemical gradient of Na 28 Mg, experiments were designed to assess whether the Na/Mg exchanger, like other gradient-driven transport systems (e.g., Na/Ca exchange and Na-K-Cl cotransporter) (46,54), is able to operate in "reverse", i.e., can mediate a Mg o -dependent Na + efflux (i.e., Na i /Mg o exchange). Assessment of this possibility was initially accomplished in internally dialyzed squid giant axons.…”
Section: + Transport In Excitable Cellsmentioning
confidence: 99%
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