Magnesium enhances dehydration tolerance in Schizosaccharomyces pombe by promoting intracellular 5′‐methylthioadenosine accumulation

Abstract

In the present study, we analysed metabolite features during the dehydration–rehydration process for two Schizosaccharomyces pombe strains with different viability rate, in order to determine whether metabolite contents were affected by the presence of magnesium during cell rehydration. The qualitative changes of the intracellular metabolites of both strains were determined by comparing the metabolic profiles of cells before dehydration, after rehydration in water or magnesium solution, and after 2‐hr inoculation of cells in complete medium. Our results suggest that changes of metabolites from the methionine salvage pathway, in particular 5′‐methylthioadenosine, triggered by the increasing intracellular magnesium content may participate in the dehydration tolerance of Sp97 cells.

Changes in Energy Status of Saccharomyces cerevisiae Cells During Dehydration and Rehydration

“…While losing the water content, yeasts synthesize the endogenous compounds, such as glycerol and trehalose [2]. A rapid change in the cellular energy metabolism reflects the higher energy demands required for surviving solutes synthesis against osmotic shock [19,20]. After ATP depletion, Marini et al [21] observed significant cytosolic compaction and extensive cytoplasmic reorganization, as well as the emergence of distinct membrane-bound and membraneless organelles.…”

“…This could be due to very active consumption of ATP by the processes of cell recovery. The efficiency of ATP hydrolysis depends on Mg 2+ ions, and these cations from the YPD medium could make ATP bioavailable [19] for many antioxidative systems to abrogate the resulting damage [2]. This could be one of the reasons why the cell reactivation in the YPD medium is more efficient than rehydration in water.…”