Magnesium promotes root nodulation through facilitation of carbohydrate allocation in soybean

Wen, Peng; Zhang, Lu Dan; Zhou, Zhigao; Chen, Fu; Chen, Zhi Chang; Liao, Hong

doi:10.1111/ppl.12730

Cited by 56 publications

(27 citation statements)

References 66 publications

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“…ARA in nodules was determined according to Peng et al (2018). Briefly, nodules were isolated with root segments left connected in order to prevent oxygen infiltration.…”

Section: Methodsmentioning

confidence: 99%

“…Ureide concentrations were determined according to Peng et al (2018). In short, basal regions of soybean shoots (2 cm above the roots) were cut and xylem sap from roots was collected with a micropipette for 1 h. The concentration of ureides in xylem sap was determined using colorimetric analysis of glyoxylate derivatives using a spectrophotometer reading at the wavelength of 525 nm (UV‐1780; Shimadzu).…”

Section: Methodsmentioning

confidence: 99%

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A VIT‐like transporter facilitates iron transport into nodule symbiosomes for nitrogen fixation in soybean

et al. 2020

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Effective legume-rhizobia symbiosis depends on efficient nutrient exchange. Rhizobia need to synthesize iron-containing proteins for symbiotic nitrogen fixation (SNF) in nodules, which depends on host plant-mediated iron uptake into the symbiosome.We functionally investigated a pair of vacuolar iron transporter like (VTL) genes, GmVTL1a/b, in soybean (Glycine max) and evaluated their contributions to SNF, including investigations of gene expression patterns, subcellular localization, and mutant phenotypes. Though both GmVTL1a/b genes were specifically expressed in the fixation zone of the nodule, GmVTL1a was the lone member to be localized at the tonoplast of tobacco protoplasts, and shown to facilitate ferrous iron transport in yeast. GmVTL1a targets the symbiosome in infected cells, as verified by in situ immunostaining. Two vtl1 knockout mutants had lower iron concentrations in nodule cell sap and peribacteroid units than in wild-type plants, suggesting that GmVTL1 knockout inhibited iron import into symbiosomes. Furthermore, GmVTL1 knockout minimally affected soybean growth under nonsymbiotic conditions, but dramatically impaired nodule development and SNF activity under nitrogen-limited and rhizobia-inoculation conditions, which eventually led to growth retardation.Taken together, these results demonstrate that GmVTL1a is indispensable for SNF in nodules as a transporter of ferrous iron from the infected root cell cytosol to the symbiosome.

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“…ARA in nodules was determined according to Peng et al (2018). Briefly, nodules were isolated with root segments left connected in order to prevent oxygen infiltration.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A VIT‐like transporter facilitates iron transport into nodule symbiosomes for nitrogen fixation in soybean

et al. 2020

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“…Suc was determined by the resorcinol method according to Peng et al (2018) using a Suc Assay Kit (Comin Biotechnology) as directed by the manufacturer. In brief, each sample was mixed with 1 mL of extract solution and heated at 80°C for 10 min.…”

Section: Suc Determinationmentioning

confidence: 99%

Magnesium Deficiency Triggers SGR–Mediated Chlorophyll Degradation for Magnesium Remobilization

et al. 2019

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Magnesium (Mg) is a relatively mobile element that is remobilized in plants under Mg-limited conditions through transport from old to young tissues. However, the physiological and molecular mechanisms underlying Mg remobilization in plants remain poorly understood. In this study, we investigated Mg remobilization in rice (Oryza sativa) as facilitated through a Mg dechelatase gene involved in chlorophyll degradation, STAY-GREEN (OsSGR). We first observed that mid-aged leaves of rice are more susceptible to Mg deficiency. Expression of OsSGR was specifically upregulated by Mg deficiency, and the response was more pronounced in mid-aged leaves. Knockout of OsSGR exhibited the stay-green phenotype, which hindered the mobility of Mg from mid-aged leaves to young developing leaves. This decline in Mg mobility was associated with inhibited growth of developing leaves in mutants under Mg-limited conditions. Furthermore, Mg deficiency enhanced reactive oxygen species (ROS) generation in mid-aged leaves. ROS levels, particularly hydrogen peroxide, in turn, positively regulated OsSGR expression, probably through chloroplast-to-nucleus signaling, which triggers chlorophyll degradation to protect mid-aged leaves from photodamage. Taken together, these results show that OsSGR-mediated chlorophyll degradation contributes to not only internal remobilization of Mg from mid-aged leaves to developing leaves, but also photooxidative protection of mid-aged leaves under Mg-limited conditions. ROS appear to act as feedback regulators of OsSGR expression to precisely govern chlorophyll degradation in mid-aged leaves where Mg and photosynthetic capacities are relatively high.

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“…Nodulation was also reported to be regulated by K and Mg in the soil. Several studies demonstrated that a higher Mg concentration elicited a greater nodule number in soybean plants [64] and that a Mg deficit induced poor nodulation in alfalfa (Medicago sativa) plants [65]. It was also stated that nodulation was inhibited under a low K supply shortly before the rhizobium invaded the root hairs [35,66].…”

Section: Root Nodulation Response To Soil N K Mg and S Based On Bimentioning

confidence: 99%

Effect of Biochar and Irrigation on the Interrelationships among Soybean Growth, Root Nodulation, Plant P Uptake, and Soil Nutrients in a Sandy Field

Egamberdieva

Wirth

et al. 2019

Sustainability

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To investigate the interrelationships among biochar, soil nutrients, and soybean plant growth in more detail, the root nodulation response of soybean (Glycine max L.) to biochar application was analyzed in a field study. We further examined the biochar effect on soil phosphatase activity to elucidate the relationships among biochar, phosphatase activity, and plant phosphorus uptake. Soybean was planted in a sandy field wherein the biochar and irrigation conditions were considered the two treatment factors. In our result, irrigation increased the pod number and plant height by 20.7% and 11.1%, respectively. Irrigation reduced the shoot and root dry matter content by 67.9% and 75.1%, respectively. The nodule number increased by 37% due to biochar addition under irrigated conditions. The soil carbon concentration was elevated by 13.4% with biochar application under rainfed conditions. Acid phosphomonoesterase (APM) was increased by 21.8% in the biochar-incorporated plots under the irrigated condition. Principal component analysis and redundancy analysis suggested that biochar application enhanced the relationships between the nodule number and soil potassium and magnesium concentrations. The correlation between soil sulfur content and nodule number was eliminated by biochar application. APM activity was associated with higher shoot and root phosphorus content and shoot dry weight after biochar application.

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Magnesium promotes root nodulation through facilitation of carbohydrate allocation in soybean

Cited by 56 publications

References 66 publications

A VIT‐like transporter facilitates iron transport into nodule symbiosomes for nitrogen fixation in soybean

A VIT‐like transporter facilitates iron transport into nodule symbiosomes for nitrogen fixation in soybean

Magnesium Deficiency Triggers SGR–Mediated Chlorophyll Degradation for Magnesium Remobilization

Effect of Biochar and Irrigation on the Interrelationships among Soybean Growth, Root Nodulation, Plant P Uptake, and Soil Nutrients in a Sandy Field

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