Magnesium Sulfate as an Alternative In Vitro Anticoagulant for the Measurement of Platelet Parameters?

Mannuß, Steffen; Schuff‐Werner, P.; Dreißiger, Katrin; Kohlschein, Peter

doi:10.1093/ajcp/aqw066

Cited by 22 publications

(25 citation statements)

References 33 publications

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“…The platelet counts, except in the heparin anti-coagulated samples, were similar, but, in contrast to the Na 2 EDTA and K 3 EDTA samples, the platelet counts in the citrate samples tended to decrease over time in some samples [19]. McShine and colleagues state that the MPV is around 6-13% lower in citrated samples as compared to the respective EDTA samples [55], which is in accordance with the observation of Dastjerdi et al, who show that the mean MPV measured from citrated samples was 0.66 fL (9%) lower than from the respective EDTA samples when measured within 1 h after sampling [56], and Mannuß et al, who found a difference of 1.32 fL (13.5%) after 180 min [8]. [58].…”

Section: Impact Of In Vitro Anticoagulation On Platelet Count and Mpvsupporting

confidence: 88%

“…After their detachment from megakaryocytes, platelets are the smallest cellular constituent of human blood. Under physiological conditions, their number is about 150,000 to 450,000 per μL of blood, and the mean platelet volume (MPV) is between 5 fL and 13 fL, depending on the applied method of measurement, the in vitro anticoagulant and the time delay between blood sampling and measurement [6,[8][9][10].…”

Section: Introductionmentioning

confidence: 99%

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Influence of different methods and anticoagulants on platelet parameter measurement

Mannuß

2020

Journal of Laboratory Medicine

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Platelets are the smallest and perhaps the most versatile components of human blood. Besides their role in coagulation and the maintenance of vascular integrity, they are involved in many physiological processes, ranging from immune response and leukocyte recruitment to the production of antimicrobial peptides and immune-suppressive factors like TGF-β. These versatile abilities make platelets interesting for researchers from different disciplines. However, beside profound investigation into platelets’ physiological role, there is a need for correct, standardized and thus reproducible quantification of platelet parameters. Mean platelet volume (MPV) is a widespread prognostic marker for several conditions, such as, acute coronary syndrome, chronic kidney disease and liver cirrhosis. Platelet activation is regarded as a marker for inflammatory processes, for example in autoimmune diseases such as type-1 diabetes, systemic lupus erythematosus and rheumatoid arthritis. The monitoring of platelet function is relevant for patients receiving antiplatelet medication. Platelet parameter measurement is affected by the choice of in vitro anticoagulant, the measurement technology and the time delay after sampling. This review focuses on the pre-analytical variability that arises as a result of the use of different in vitro anticoagulants and analyzer technologies when determining platelet parameters, since, even approximately 180 years after the discovery of platelets, there is still no standardized procedure.

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Section: Impact Of In Vitro Anticoagulation On Platelet Count and Mpvsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Influence of different methods and anticoagulants on platelet parameter measurement

Mannuß

2020

Journal of Laboratory Medicine

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“…As a result, these methods do not agree with each other. Sample storage time is another parameter which influences MPV [75]. Although not all studies stated sample storage time in their investigation, MPV was significantly higher than controls in both ≤120 min and >120 min groups.…”

Section: Discussionmentioning

confidence: 86%

Platelet count and mean volume in acute stroke: a systematic review and meta-analysis

Sadeghi

Sándor

Zsóri³

et al. 2019

Platelets

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Changes of mean platelet volume (MPV) and platelet count (PC) could be a marker or a predictor of acute stroke (AS). We conducted a systematic review and meta-analysis of the published literature on the reporting of MPV and PC in AS. Studies were included in accordance with Patient Population or Problem, Intervention, Comparison, Outcomes, and Setting framework. The PRISMA strategy was used to report findings. Risk of bias was assessed with the Newcastle-Ottawa Scale. We included 34 eligible articles retrieved from the literature. PC was significantly lower in AS patients [standardized mean difference (SMD) = − 0.30, (95% CI: − 0.49 to − 0.11), N = 2492, P = .002] compared with controls (N = 3615). The MPV was significantly higher [SMD = 0.52 (95% CI: 0.28-0.76), N = 2739, P < .001] compared with controls (N = 3810). Subgroup analyses showed significantly lower PC in both ischemic stroke (Difference SMD = −0.18, 95% CI: −0.35-0.01) and hemorrhagic stroke (−0.94, −1.62 to −0.25), but only samples by citrate anticoagulant showed significantly lower result for patients compared to controls (−0.36, −0.68 to −0.04). Ischemic stroke patients had higher MPV (0.57, 0.31-0.83), and samples by Ethylenediaminetetraacetic acid (EDTA) anticoagulant showed significantly higher result for patients compared to controls (0.86, 0.55--1.17). PC and MPV appeared to be significantly different between patients with AS and control populations. MPV was significantly higher in ischemic stroke and PC was significantly lower in both ischemic and hemorrhagic strokes. These characteristics might be related to AS and associated with it. It is advisable to pay attention to elapsed time between phlebotomy and hematology analysis, anticoagulant and hemocytometer types in AS. Systematic review registration: This meta-analysis is registered on the International Prospective Register of Systematic Reviews (PROSPERO) under registration number CRD42017067864 (https:// www.crd.york.ac.uk/prospero/display_record.php?RecordID=67864).

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“…Mean platelet volume is routinely measured in blood samples anticoagulated with tripotassium-ethylenediaminetetraacetic acid (K3EDTA), collected for complete blood count. MPV increases in K3EDTA samples with time, due to platelet swelling [6][7][8][9][10][11] : As a consequence, the timing of MPV measurement after blood sampling should be standardized to allow accurate comparison of the values obtained in the same laboratory and across different laboratories. Lack of standardization of this important pre-analytical variable, in combination with different performances of hematology analyzers explain the wide interlaboratory variability of MPV normal ranges.…”

Section: In Vitro Variations Of Mean Platelet Volume Over Time In Blomentioning

confidence: 99%

“…8,12,13 With the aim of reducing the pre-analytical variability associated with K3EDTAinduced platelet swelling, anticoagulants different from K3EDTA have been tested and proposed as potentially useful for assessing platelet indices. 9,14 To identify the ideal pre-analytical variables for measuring MPV, we compared its variation over time in blood samples collected in 4 different anticoagulants from the same normal subjects and patients with inherited or acquired thrombocytopenias. The mean platelet count (±SD) was 252 × 10 9 /L (±64) in blood donors and 75 × 10 9 /L (±35) in thrombocytopenic subjects.…”

Section: In Vitro Variations Of Mean Platelet Volume Over Time In Blomentioning

confidence: 99%

In vitro variations of Mean platelet volume over time in blood samples collected in different anticoagulants from thrombocytopenic patients and healthy subjects

Podda

Casazza

Soru

et al. 2017

Int J Lab Hematology

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Magnesium Sulfate as an Alternative In Vitro Anticoagulant for the Measurement of Platelet Parameters?

Abstract: Magnesium anticoagulation might be advantageous for more reliable MPV measurements. Although platelet count is underestimated when the impedance method is used, the platelet count reveals similar results when measured by the fluorescent optical method.

Cited by 22 publications

References 33 publications

Influence of different methods and anticoagulants on platelet parameter measurement

Influence of different methods and anticoagulants on platelet parameter measurement

Platelet count and mean volume in acute stroke: a systematic review and meta-analysis

In vitro variations of Mean platelet volume over time in blood samples collected in different anticoagulants from thrombocytopenic patients and healthy subjects

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