Magnet-induced behavior of iron carbide (Fe7C3@C) nanoparticles in the cytoplasm of living cells

Alieva, Irina B.; Kireev, Igor I.; Rakhmanina, A. V.; Garanina, Anastasiia S.; Strelkova, Olga; Zhironkina, Oxana A.; Cherepaninets, V. D.; Davydov, V. A.; Khabashesku, Valéry N.; Агафонов, В.; Uzbekov, Rustem

doi:10.17586/2220-8054-2016-7-1-158-160

Cited by 6 publications

(5 citation statements)

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“…In our previous work we suggested that at least part of MNPs is localized inside the endosomes [ 16 , 18 ]. To confirm these observations we studied colocalization of cytoplasmic agglomerates of MNPs with endosomes immunostained for endosomal marker Rab5 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Magnetocontrollability of Fe7C3@C superparamagnetic nanoparticles in living cells

et al. 2016

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BackgroundA new type of superparamagnetic nanoparticles with chemical formula Fe7C3@C (MNPs) showed higher value of magnetization compared to traditionally used iron oxide-based nanoparticles as was shown in our previous studies. The in vitro biocompatibility tests demonstrated that the MNPs display high efficiency of cellular uptake and do not affect cyto-physiological parameters of cultured cells. These MNPs display effective magnetocontrollability in homogeneous liquids but their behavior in cytoplasm of living cells under the effect of magnetic field was not carefully analyzed yet.ResultsIn this work we investigated the magnetocontrollability of MNPs interacting with living cells in permanent magnetic field. It has been shown that cells were capable of capturing MNPs by upper part of the cell membrane, and from the surface of the cultivation substrate during motion process. Immunofluorescence studies using intracellular endosomal membrane marker showed that MNP agglomerates can be either located in endosomes or lying free in the cytoplasm. When attached cells were exposed to a magnetic field up to 0.15 T, the MNPs acquired magnetic moment and the displacement of incorporated MNP agglomerates in the direction of the magnet was observed. Weakly attached or non-attached cells, such as cells in mitosis or after cytoskeleton damaging treatments moved towards the magnet. During long time cultivation of cells with MNPs in a magnetic field gradual clearing of cells from MNPs was observed. It was the result of removing MNPs from the surface of the cell agglomerates discarded in the process of exocytosis.ConclusionsOur data allow us to conclude for the first time that the magnetic properties of the MNPs are sufficient for successful manipulation with MNP agglomerates both at the intracellular level, and within the whole cell. The structure of the outer shells of the MNPs allows firmly associate different types of biological molecules with them. This creates prospects for the use of such complexes for targeted delivery and selective removal of selected biological molecules from living cells.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-016-0219-4) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Magnetocontrollability of Fe7C3@C superparamagnetic nanoparticles in living cells

et al. 2016

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“…These objects can be used, for instance, as shells or cores of nanocapsules, providing the release of drugs, for example, under local electromagnetic influence. They can play the role of carriers of bioactive substances in surface functionalization [44-46, 48, 49, 51-56], or when the porous structure of nanoparticles and clusters is used, including non-magnetic and magnetic systems for delivery, therapy and diagnostics [60][61][62][63][64][65][66][67][68]. The use of carbon clusters and inorganic nanoparticles is associated with the problem of removing such materials from the body after they have performed their function, in order to reduce the xenobiotics load.…”

Section: Nanoscale Components Of Targeted Drug Delivery Systemsmentioning

confidence: 99%

Physicochemical and biochemical properties of the Keplerate-type nanocluster polyoxomolybdates as promising components for biomedical use

Ostroushko¹,

Grzhegorzhevskii²,

Medvedeva³

et al. 2021

Nanosystems: Phys. Chem. Math.

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The paper discusses the results of a research on physicochemical and biochemical properties of the Keplerate-type molybdenum-based nanocluster polyoxometalates (POMs), which show promise in the field of biomedicine as a means of targeted drug delivery, including the transport to immune privileged organs. POMs can be considered as components of releasing systems, including the long-acting ones with feedback (for controlling the drug active component release rate). POMs are promising drugs for the treatment of anemia. Also, the paper deals with the results of studies of POM effect on living systems at the molecular and cellular levels, at that of individual organs, and on the organism as a whole. The mechanism and kinetics of POM destruction and possibilities of stabilization, the oscillatory phenomena manifestation, the formation of POM conjugates with bioactive substances which can be released during the destruction of POM, with polymer components, and with indicator fluorescent dyes, as well as forecasts for further research, are considered.

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“…Nanoparticles of iron carbides Fe 7 C 3 and Fe 3 C, encapsulated into carbon shells, are characterized by high biocompatibility and can be controlled by an external magnetic field. Hence, they can be considered as new-type platforms for biomedical applications [1].…”

Section: Introductionmentioning

confidence: 99%

Thermal Transformations of Ferrocene Fe(C5H5)2 at a Pressure of 10 GPa and Temperatures up to 2200 K

Starchikov,

Zayakhanov,

Troyan

et al. 2024

Jetp Lett.

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Specific features of thermal transformations of ferrocene Fe(C5H5)2 at a pressure of 10 GPa upon laser heating to 2200 K have been investigated in diamond anvil cells. Maps of the temperature distribution on the sample during the heating have been obtained. The structure and properties of the transformation products have been studied by X-ray diffraction analysis, transmission electron microscopy, and Mössbauer spectroscopy. It is established that a characteristic feature of ferrocene transformations upon laser heating is the simultaneous formation of nanoparticles of iron (α-Fe) and iron carbide (Fe7C3) crystalline phases. The presence of α-Fe in the products of thermal transformations of ferrocene at high pressures has been observed for the first time. Possible mechanisms of the simultaneous formation of these nanoparticles during ferrocene transformations are discussed.

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Magnet-induced behavior of iron carbide (Fe7C3@C) nanoparticles in the cytoplasm of living cells

Cited by 6 publications

References 1 publication

Magnetocontrollability of Fe7C3@C superparamagnetic nanoparticles in living cells

Magnetocontrollability of Fe7C3@C superparamagnetic nanoparticles in living cells

Physicochemical and biochemical properties of the Keplerate-type nanocluster polyoxomolybdates as promising components for biomedical use

Thermal Transformations of Ferrocene Fe(C5H5)2 at a Pressure of 10 GPa and Temperatures up to 2200 K

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