Earth's gravity exerts relatively weak forces in the range of 10 -100 pN directly on cells in biological systems. Nevertheless, it biases the orientation of swimming unicellular organisms, alters bone cell differentiation, and modifies gene expression in renal cells. A number of methods of simulating different strength gravity environments, such as centrifugation, have been applied for researching the underlying mechanisms. Here, we demonstrate a magnetic force-based technique that is unique in its capability to enhance, reduce, and even invert the effective buoyancy of cells and thus simulate hypergravity, hypogravity, and inverted gravity environments. We apply it to Paramecium caudatum, a single-cell protozoan that varies its swimming propulsion depending on its orientation with respect to gravity, g. In these simulated gravities, denoted by f gm, Paramecium exhibits a linear response up to fgm ؍ 5 g, modifying its swimming as it would in the hypergravity of a centrifuge. Moreover, experiments from f gm ؍ 0 to ؊5 g show that the response is symmetric, implying that the regulation of the swimming speed is primarily related to the buoyancy of the cell. The response becomes nonlinear for f gm >5 g. At fgm ؍ 10 g, many paramecia ''stall'' (i.e., swim in place against the force), exerting a maximum propulsion force estimated to be 0.7 nN. These findings establish a general technique for applying continuously variable forces to cells or cell populations suitable for exploring their force transduction mechanisms.buoyancy ͉ force ͉ gravikinesis ͉ levitation ͉ microorganism