Magnetic‐activated cell sorting before density gradient centrifugation improves recovery of high‐quality spermatozoa

Berteli, Thalita S.; Broi, Michele Gomes Da; Martins, W.P.; Ferriani, Rui Alberto; Navarro, Paula Andrea

doi:10.1111/andr.12372

Cited by 35 publications

(29 citation statements)

References 42 publications

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“…Cakar et al (2016) did not find significant differences in any of the analysis of sperm quality, including DNA fragmentation, by using AV-MACS, SU, DG, SU/AV-MACS, and DG/AV-MACS, perhaps due to the low sample size of the study. In contrast, Berteli et al (2017) found a better quality in terms of motility and DNA fragmentation in spermatozoa selected by AV-MACS followed by DCG than with the other combinations tested: DCG and AV-MACS alone or DCG followed by AV-MACS. Similar to these results, Zhang et al (2018) reported that the combination of DCG followed by AV-MACS in immotile sperm samples resulted in the recovery of a sperm population with lower DNA fragmentation compared to DGC alone.…”

Section: Sperm Selection Based On Membrane Characteristicsmentioning

confidence: 63%

Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes

Oseguera-López

Ruiz-Díaz

Ramos‐Ibeas

et al. 2019

Front. Cell Dev. Biol.

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Almost 50% of the infertility cases are due to male factors. Assisted reproductive technologies (ARTs) allow to overcome the incapacity of these patients' spermatozoa to fertilize the oocyte and produce a viable and healthy offspring, but the efficiency of the different techniques has still the potential to improve. According to the latest reports of the European Society of Human Reproduction and Embryology (ESHRE) and the Centers for Disease Control and Prevention of the United States (CDC), the percentages of deliveries per ART cycle in 2014 and 2016 were 21 and 22%, respectively. Among the reasons for this relatively low efficiency, the quality of the spermatozoa has been pointed out as critical, and the presence of high percentages of DNA-damaged spermatozoa in patients' ejaculates is possibly one of the main factors reducing the ARTs outcomes. Thus, one of the main challenges in reproductive medicine is to ensure the highest quality of the spermatozoa used in ARTs, and specifically, in terms of genetic integrity. The latest techniques for the preparation and selection of human spermatozoa are herein discussed focusing on those proven to improve one or several of the following parameters: sperm genetic integrity, fertilization capacity, embryo production, and in vitro survival, as well as pregnancy and delivery rates following in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). In addition, we discuss the potential of techniques developed in non-human mammals that could be further transferred to the clinic.

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Section: Sperm Selection Based On Membrane Characteristicsmentioning

confidence: 63%

Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes

Oseguera-López

Ruiz-Díaz

Ramos‐Ibeas

et al. 2019

Front. Cell Dev. Biol.

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“…As the correlation between DNA damage and apoptosis with the consequent externalisation of phosphatidylserine is well established (Muratori et al, 2003), it is no surprise that several studies reported that the spermatozoa retrieved with MACS have reduced levels of DNA fragmentation (Berteli, Da Broi, Martins, Ferriani, & Navarro, 2017;Lee et al, 2010;Rawe et al, 2009;Said et al, 2006;Zhang et al, 2018). In addition, the retrieved spermatozoa exhibit higher motility (Romany et al, 2014;Said et al, 2005Said et al, , 2006 and higher percentage of spermatozoa with normal morphology (Berteli et al, 2017;Zhang et al, 2018). Although with MACS protocol it is possible to obtain better spermatozoa, the published data concerning clinical outcomes in ART are still inconclusive.…”

Section: Magnetic-activated Cell Sortingmentioning

confidence: 99%

Sperm selection strategies and their impact on assisted reproductive technology outcomes

et al. 2020

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Assisted reproductive technologies (ART) revolutionised human fertility by providing treatment to patients previously considered incapable for reproductive purposes (Henkel & Schill, 2003). In 2017, it was estimated that approximately 6 million infants were born through ART since 1978, when the first baby produced by in vitro fertilisation (IVF) was born. It has been reported that about half of these births were in the past decade, highlighting the increasing use of these technologies, (Hunter, 2017). Initially, ART research was mainly focused on comprehending the factors that increase the likelihood of a successful pregnancy and

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“…MACS was used to separate sperm into 2 populations: an annexin V (-) fraction (spermatozoa without EPS) and an annexin V (+) fraction (spermatozoa with EPS) (de Vantéry Arrighi et al 2009;Grunewald et al 2009;Huang et al 2009;Buzzi et al 2010). This is a very simple, specific and rapid technique which is known to reduce a large proportion of apoptotic spermatozoa in the processed samples (Said et al 2006), and also to reduce the level of DNA fragmentation (Rawe et al 2009;Lee et al 2010;Herrero et al 2012;Delbes et al 2013;Nadalini et al 2014;Troya & Zorrilla 2015;Berteli et al 2017). As a consequence, spermatozoa that have undergone abortive apoptosis should be retained in the column when using annexin V coupled with MACS.…”

Section: Introductionmentioning

confidence: 99%

Decrease of spermatozoa with an unbalanced chromosome content after cell sorting in men carrying a structural chromosomal abnormality

et al. 2019

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Background: We showed that in men with a constitutional chromosomal abnormality, DNA fragmentation was significantly higher in chromosomally unbalanced spermatozoa than in spermatozoa with a normal or balanced chromosomal content. These results could be explained by a phenomenon already described in infertile men: abortive apoptosis. Objectives: To determine if magnetic-activated cell separation could select spermatozoa with lower levels of DNA fragmentation and unbalanced chromosome content in men carrying a structural chromosomal abnormality. Materials and methods: The spermatozoa of ten males with a chromosomal rearrangement were separated into two populations using MACS (annexin V (-) and annexin V (+) fractions), in order to study meiotic segregation by FISH, the percentage of spermatozoa with an externalization of phosphatidylserine (EPS) by annexin V staining and DNA fragmentation by TUNEL on the whole ejaculate and on selected spermatozoa in the same patient. Results: For all patients, the percentage of spermatozoa with EPS decreased in the annexin V (-) fraction and increased in the annexin V (+) fraction as compared to the frozen-thawed semen sample. The rates of DNA fragmentation were statistically much lower in the annexin V (-) fraction when compared to the rate before MACS for all but one patient. Conversely, we observed a statistically significant higher rate of DNA fragmentation in the annexin V (+) fraction for 6 patients. After MACS, there was a significant increase of normal/balanced spermatozoa in the fraction of annexin V (-) for all patients. Conversely, we observed a significant decrease in the fraction of annexin V (+) for seven patients. Discussion and Conclusions: MACS is a promising tool for increasing the selection of healthy spermatozoa, with a decrease in the number of spermatozoa with EPS, DNA fragmentation and chromosome unbalance, for use in assisted reproductive technologies such as ICSI for males with a chromosomal structural abnormality.

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Magnetic‐activated cell sorting before density gradient centrifugation improves recovery of high‐quality spermatozoa

Cited by 35 publications

References 42 publications

Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes

Novel Techniques of Sperm Selection for Improving IVF and ICSI Outcomes

Sperm selection strategies and their impact on assisted reproductive technology outcomes

Decrease of spermatozoa with an unbalanced chromosome content after cell sorting in men carrying a structural chromosomal abnormality

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