Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained infertility

Lee, Tsung-Hsien; Liu, Chung‐Hsien; Shih, Yung‐Feng; Tsao, Hui‐Mei; Huang, Chun‐Chia; Chen, Hsiu‐Hui; Lee, Maw‐Sheng

doi:10.1093/humrep/deq009

Cited by 107 publications

(82 citation statements)

References 31 publications

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“…Similarly, the changes in MMP already observed in cases of inflammation [9,35,40,47] were correlated with FSH, the main hormonal regulator of spermatogenesis; however, this relation did not exist with DNA denaturation, which is in agreement with the findings of Appasamy et al [1]. We have observed a highly significant correlation between dead, apoptotic and viable sperm, viable sperm with normal MMP, sperm with DNA denaturation and conventional sperm parameters, which is in agreement with the findings in the literature [3,6,27,41].…”

Section: Discussionsupporting

confidence: 92%

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Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Zorn

Golob

Ihan

et al. 2012

J Assist Reprod Genet

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Purpose To investigate the relationship between sperm apoptotic biomarkers and patient clinical characteristics, conventional sperm parameters and fertility potential. Material and methods Sperm analysis, phospholipid asymmetry, mitochondrial membrane potential (MMP) and DNA denaturation were assessed in 142 males of infertile couples. Seventy-three couples were allocated to the natural conception group, and 55 couples underwent IVF or ICSI. Results DNA denaturation correlated positively with age and negatively with testicular volume (TV). MMP correlated negatively with BMI and FSH and positively with TV. Normal viable sperm correlated positively with TV and negatively with age, BMI and FSH. DNA denaturation was associated with a significantly lower natural pregnancy rate (OR 5.4, p00.011). Conclusion Sperm apoptosis is related to male age, BMI, testicular volume and FSH. Among the apoptotic markers, only DNA denaturation has been found to predict natural pregnancy better than conventional sperm parameters.

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Section: Discussionsupporting

confidence: 92%

“…High quality non-apoptotic sperm, characterized by low levels of DNA damage, demonstrate high sperm capacitation [19], improved acrosome reaction [27] and oocyte penetration capacity [37].…”

Section: Discussionmentioning

confidence: 99%

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Zorn

Golob

Ihan

et al. 2012

J Assist Reprod Genet

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“…In fact, Annexin V staining revealed more viable cells in normozoospermic patients (Varum et al 2007) and seemed to correlate with sperm parameters in other studies (Shen et al 2002, Weng et al 2002. Importantly, the use of magnetic activated cell sorting (MACS) with Annexin V microbeads to select sperm reduced the percentage of altered cells (Lee et al 2010, Rawe et al 2010, Tavalaee et al 2012. On the other hand, the presence of activated caspases (the final step in apoptosis) has also been linked to poor sperm quality and lower fertilisation potential, possibly by affecting sperm DNA (Weng et al 2002, Grunewald et al 2008, Kotwicka et al 2008, both in the case of caspase 9 (activated by the mitochondrial pathway of apoptosis following cytochrome c release) and caspase 3 (activated by both apoptotic pathways).…”

Section: Apoptosismentioning

confidence: 80%

Mitochondria functionality and sperm quality

Amaral¹,

Lourenço²,

Marques³

et al. 2013

Reproduction

448

332

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Although mitochondria are best known for being the eukaryotic cell powerhouses, these organelles participate in various cellular functions besides ATP production, such as calcium homoeostasis, generation of reactive oxygen species (ROS), the intrinsic apoptotic pathway and steroid hormone biosynthesis. The aim of this review was to discuss the putative roles of mitochondria in mammalian sperm function and how they may relate to sperm quality and fertilisation ability, particularly in humans. Although paternal mitochondria are degraded inside the zygote, sperm mitochondrial functionality seems to be critical for fertilisation. Indeed, changes in mitochondrial integrity/functionality, namely defects in mitochondrial ultrastructure or in the mitochondrial genome, transcriptome or proteome, as well as low mitochondrial membrane potential or altered oxygen consumption, have been correlated with loss of sperm function (particularly with decreased motility). Results from genetically engineered mouse models also confirmed this trend. On the other hand, increasing evidence suggests that mitochondria derived ATP is not crucial for sperm motility and that glycolysis may be the main ATP supplier for this particular aspect of sperm function. However, there are contradictory data in the literature regarding sperm bioenergetics. The relevance of sperm mitochondria may thus be associated with their role in other physiological features, particularly with the production of ROS, which in controlled levels are needed for proper sperm function. Sperm mitochondria may also serve as intracellular Ca 2C stores, although their role in signalling is still unclear.

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“…Selection of high-quality spermatozoa via flow cytometry (Paasch et al 2007) magnetic sorting (Said et al 2008) or microfluidic sorting (Wu et al 2006) has proven useful for human ART (Lee et al 2010) and it is even possible to sort viable sperm cells that are responsive to capacitation from those that are not (de Vries et al 2003). Despite these possibilities, sorting is only routinely applied to separate X and Y chromosomebearing spermatozoa for the production of offspring with pre-selected sex.…”

Section: Sex-sortingmentioning

confidence: 99%

Sperm surface changes and physiological consequences induced by sperm handling and storage

2011

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Spermatozoa interact with their immediate environment and this contact remodels the sperm surface in preparation for fertilisation. These fundamental membrane changes will be critically covered in this review with special emphasis on the very specific surface destabilisation event, capacitation. This process involves very subtle and intricate modifications of the sperm membrane including removal of suppression (decapacitation) factors and changes in the lateral organisation of the proteins and lipids of the sperm surface. Processing of sperm for assisted reproduction (storage, sex-sorting, etc.) subjects spermatozoa to numerous stressors, and it is possible that this processing overrides such delicate processes resulting in sperm instability and cell damage. To improve sperm quality, novel mechanisms must be used to stabilise the sperm surface during handling. In this review, different types of membrane stress are considered, as well as novel surface manipulation methods to improve sperm stability.

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Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained infertility

Cited by 107 publications

References 31 publications

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Apoptotic sperm biomarkers and their correlation with conventional sperm parameters and male fertility potential

Mitochondria functionality and sperm quality

Sperm surface changes and physiological consequences induced by sperm handling and storage

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