Magnetic anisotropy and spin fluctuations in the paramagnetic dimer : Mössbauer spectroscopy and ab initio calculations

Cianchi, L.; Giallo, F. Del; Moretti, Paolo; Spina, G.; Mancini, M.; Caneschi, Andréa; Lantieri, Marco

doi:10.1016/j.jmmm.2007.09.015

Cited by 1 publication

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The spectra were simulated and fit using WMoss (SeeCo) with Lorentzian doublets. The fits are anisotropic as a result of the clusters' paramagnetism affecting the relaxation time of the transitions (58,59).…”

Section: Methodsmentioning

confidence: 99%

A [3Fe-4S] cluster is required for tRNA thiolation in archaea and eukaryotes

Liu

Vinyard

Reesbeck

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The sulfur-containing nucleosides in transfer RNA (tRNAs) are present in all three domains of life; they have critical functions for accurate and efficient translation, such as tRNA structure stabilization and proper codon recognition. The tRNA modification enzymes ThiI (in bacteria and archaea) and Ncs6 (in archaea and eukaryotic cytosols) catalyze the formation of 4-thiouridine (s 4 U) and 2-thiouridine (s 2 U), respectively. The ThiI homologs were proposed to transfer sulfur via cysteine persulfide enzyme adducts, whereas the reaction mechanism of Ncs6 remains unknown. Here we show that ThiI from the archaeon Methanococcus maripaludis contains a [3Fe-4S] cluster that is essential for its tRNA thiolation activity. Furthermore, the archaeal and eukaryotic Ncs6 homologs as well as phosphoseryl-tRNA (Sep-tRNA):Cys-tRNA synthase (SepCysS), which catalyzes the Sep-tRNA to Cys-tRNA conversion in methanogens, also possess a [3Fe-4S] cluster similar to the methanogenic archaeal ThiI. These results suggest that the diverse tRNA thiolation processes in archaea and eukaryotic cytosols share a common mechanism dependent on a [3Fe-4S] cluster for sulfur transfer.iron-sulfur cluster | thionucleosides | tRNA modification | CTU1

show abstract

Section: Methodsmentioning

confidence: 99%