Magnetic antifouling material based ratiometric electrochemical biosensor for the accurate detection of CEA in clinical serum

Yang, Huiqing; Xu, Yan; Hou, Qianqian; Xu, Qingzhang; Ding, Caifeng

doi:10.1016/j.bios.2022.114216

Cited by 56 publications

(12 citation statements)

References 38 publications

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“…It is well-known that the concentration of Fc-labeled ssDNA is also one of the critical factors affecting the sensitivity of the biosensor [ [64] , [65] , [66] ]. An excess of Fc-labeled ssDNA may result in shedding from the SPCE and impact the electrochemical signal.…”

Section: Resultsmentioning

confidence: 99%

Ultrasensitive SARS-CoV-2 diagnosis by CRISPR-based screen-printed carbon electrode

Wang²,

Wu³

et al. 2022

Analytica Chimica Acta

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Section: Resultsmentioning

confidence: 99%

Ultrasensitive SARS-CoV-2 diagnosis by CRISPR-based screen-printed carbon electrode

Wang²,

Wu³

et al. 2022

Analytica Chimica Acta

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“…The following supporting information [4,[23][24][25][26][27][28][29] can be downloaded at: https://www.mdpi.com/article/10.3390/bios12070514/s1, Table S1: Optimization of the experimental conditions for the assembly and use of the amperometric aptasensor for CEA. ; Figure S1: Cyclic voltammograms for Apt-ADA/AuNF/GO/SPE before (a) and after sequential incubation with 20 pg/mL CEA (b) and HRP-CD (c), measured in 0.1 M KCl solution containing 5 mM K 3 [Fe(CN) 6 ]/ K 4 [Fe(CN) 6 ] (1:1).…”

Section: Discussionmentioning

confidence: 99%

Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor

et al. 2022

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Here we report a novel labeling strategy for electrochemical aptasensors based on enzymatic marking via supramolecular host–guest interactions. This approach relies on the use of an adamantane-modified target-responsive hairpin DNA aptamer as an affinity bioreceptor, and a neoglycoconjugate of β-cyclodextin (CD) covalently attached to a redox enzyme as a labeling element. As a proof of concept, an amperometric aptasensor for a carcinoembryonic antigen was assembled on screen-printed carbon electrodes modified with electrodeposited fern-like gold nanoparticles/graphene oxide and, by using a horseradish peroxidase-CD neoglycoenzyme as a biocatalytic redox label. This aptasensor was able to detect the biomarker in the concentration range from 10 pg/mL to 1 ng/mL with a high selectivity and a low detection limit of 3.1 pg/mL in human serum samples.

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“…Due to the merits of large specific surface area, good reducibility, and biocompatibility, MXenes have been combined with other materials to improve electrochemical sensing performances. Yang et al 67 constructed an aptamer sensor based on MXene to detect CEA with different concentrations. The construction steps of the sensor are shown in Figure 3a .…”

Section: Applications Of Electrochemical Biosensors In Leukemiamentioning

confidence: 99%

“…Inspired by these, some relevant biomolecules associated with severe HMs have been investigated by many research teams based on electrochemical biosensors. 2 , 8 , 65 – 68 Hence, we present a brief review of the state-of-the-art electrochemical biosensors based on 2D materials (graphene, TMDs, MXenes, g-C 3 N 4 , MOFs, or BP, etc) and their hybrid composites in the detection of HMs related biomolecules ( Scheme 1 ). In addition, current limitations and the prospect of using 2D materials-based materials electrochemical biosensors in HMs diagnosis are discussed, which may bring new opportunities for the design and development of high-performance 2D materials-based electrochemical biosensors in the HMs diagnosis field.…”

Section: Introductionmentioning

confidence: 99%

Applications of electrochemical biosensors based on 2D materials and their hybrid composites in hematological malignancies diagnosis

Sun

Huang

Wang

et al. 2022

Technol Cancer Res Treat

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Hematological malignancies encompass a wide variety of severe diseases that pose a serious threat to human health. Given the fact that hematological malignancies are difficult to treat due to their unpredictable and rapid deterioration and high rates of recurrence, growing attention has been paid to their early screening and diagnosis. However, developing a rapid and effective diagnostic tool featuring a noninvasive sampling technique is still extremely challenging. In recent years, novel nanomaterials-based electrochemical biosensors have attracted great interest because of such advantages as simple operation, low cost, fast response, etc. As a kind of rising nanomaterials, two-dimensional materials have excellent electronic and chemical properties, which have been proven to improve the performance of electrochemical biosensors. This review summarizes the applications of different types of electrochemical biosensors (nucleic acid sensors, immunosensors, aptamer biosensors, and cytosensors) based on two-dimensional materials in the detection of biological molecules related to hematological malignancies. Two-dimensional materials-based electrochemical biosensors designed for the diagnosis of leukemia could rapidly detect the target biomolecules at a trace level and show great merits such as wide linear range, low detection limit, high sensitivity, excellent selectivity, and cost-effectiveness. In addition, these biosensors have also achieved satisfactory results in the diagnosis of lymphoma and multiple myeloma. Thus, two-dimensional materials-based electrochemical biosensors are attractive for the early diagnosis of hematological malignancies in clinical practice. Nevertheless, more efforts are still required to further improve the performance of electrochemical biosensors. In this review, we propose the possible main concerns in the design of future two-dimensional materials-based electrochemical biosensors, involving the development of sensors for synchronous detection of diverse target biomolecules, the exploration of other superior two-dimensional materials, the simplification of the sensors fabrication process, the construction of new hybrid structures and how to avoid possible environmental issues.

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Magnetic antifouling material based ratiometric electrochemical biosensor for the accurate detection of CEA in clinical serum

Cited by 56 publications

References 38 publications

Ultrasensitive SARS-CoV-2 diagnosis by CRISPR-based screen-printed carbon electrode

Ultrasensitive SARS-CoV-2 diagnosis by CRISPR-based screen-printed carbon electrode

Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor

Applications of electrochemical biosensors based on 2D materials and their hybrid composites in hematological malignancies diagnosis

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