Magnetic assistance highly sensitive protein assay based on surface-enhanced resonance Raman scattering

Cover Picture: Dispersion of HiPco® and CoMoCAT® Single‐Walled Nanotubes (SWNTs) by Water Soluble Pyrene Derivatives—Depletion of Small Diameter SWNTs (Chem. Eur. J. 11/2010)

“…Immunoassays of antigens and Escherichia coli ( E. coli ) were carried out by combining antibody‐conjugated Au nanoparticles labeled with Raman reporters and antibody‐conjugated γ ‐Fe 2 O 3 @Au or Fe 3 O 4 @Au nanoparticles 5a, 6. A simple SERS‐based protocol for the detection of protein‐small molecule interactions was developed by combining avidin‐immobilized Fe 3 O 4 @Ag nanoparticles and Raman reporter‐labeled biotin 7. Although the nanoparticles with magnetic cores and noble metal shells may give strong SERS signals probably due to the generation of “hot spots” as well as magnetic separation, the noble metal shells ultimately cannot resist to high‐concentration salts under physiological conditions, which lead to aggregation and sediment of nanoparticles.…”

Optical-Based (Bio) Sensing Systems Using Magnetic Nanoparticles

“…These improvements can be achieved using MNPs or magnetic nanocomposites because of their unique properties such as strong superparamagnetic property, low toxicity, biocompatibility, easy preparation, and high adsorption ability. As outlined in Table 2, MNPs in SERS analysis have been used for the detection of proteins [65][66][67][68], cells [69][70][71], toxins [72], drugs [73,74], microorganisms [75][76][77][78], illegal additives [79,80], antigens [81,82], pesticides [83], and genes [84,85]. As mentioned above, gold (Au) nanostructures in the manufacture of SERS substrates are one of the most widely used metallic nanostructures for the plasmonic substrate.…”

A Review of Chinese Raman Spectroscopy Research Over the Past Twenty Years

“…It mainly focuses on the SERS of protein, and the preparation and application research upon biocompatible substrates to achieve protein label-free detection or magnetic-assisted highly sensitive detection. 26 In recent years, this group has been studying precious-metal-ordered microstructured nanoparticle self-assembly. The polymer is coated with avidin and is subsequently injected into the gold-decorated polymer using a buffer solution to block the areas without avidin not specifically adsorbed onto the substrate, and 1 mL of different concentrations of an Atto 610-Biotin solution was injected for specific recognition over a 10 minute period.…”