Magnetic bead-based chemiluminescence detection of sequence-specific DNA by using catalytic nucleic acid labels

Cao, Zhijuan; Li, Zengxi; Zhao, Yujie; Song, Yanyan; Lu, Jianzhong

doi:10.1016/j.aca.2005.10.048

Cited by 37 publications

(24 citation statements)

References 32 publications

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“…It should be noted that ABTS is not an ideal substrate because ABTS + forming on the oxidation of ABTS is not a stable compound and quickly disproportionates with the production of colorless compounds [26,27]. Of the above-mentioned methods, the CL method, which is based on PMDNAzyme-catalyzed oxidation of luminol with a generation of light, is the most sensitive [22][23][24].…”

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confidence: 98%

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Improved method for chemiluminescent determination of peroxidase-mimicking DNAzyme activity

Gribas

Zhao

Sakharov

2014

Analytical Biochemistry

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confidence: 98%

“…To determine the activity of PMDNAzyme, colorimetric, fluorimetric, and chemiluminescent (CL) methods are applied [20][21][22][23][24].…”

mentioning

confidence: 99%

Improved method for chemiluminescent determination of peroxidase-mimicking DNAzyme activity

Gribas

Zhao

Sakharov

2014

Analytical Biochemistry

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“…Paramagnetic or superparamagnetic particles/beads (MPs) represents promising tool in this field [44][45][46]. MPs, whose size is ranging from nm to mm, respond to external magnetic field and facilitate bioactive molecules binding because of their affinity for the MPs modified surface made of biologically components [47][48][49][50].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic tool based on the antibody‐modified paramagnetic particles for detection of 8‐hydroxy‐2′‐deoxyguanosine in urine of prostate cancer patients

et al. 2011

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Guanosine derivatives are important for diagnosis of oxidative DNA damage including 8-hydroxy-2'-deoxyguanosine (8-OHdG) as one of the most abundant products of DNA oxidation. This compound is commonly determined in urine, which makes 8-OHdG a good non-invasive marker of oxidation stress. In this study, we optimized and tested the isolation of 8-OHdG from biological matrix by using paramagnetic particles with an antibody-modified surface. 8-OHdG was determined using 1-naphthol generated by alkaline phosphatase conjugated with the secondary antibody. 1-Naphthol was determined by stopped flow injection analysis (SFIA) with electrochemical detector using a glassy carbon working electrode and by stationary electrochemical detection using linear sweep voltammetry. A special modular electrochemical SFIA system which needs only 10 μL of sample including working buffer for one analysis was completely designed and successfully verified. The recoveries in different matrices and analyte concentration were estimated. Detection limit (3 S/N) was estimated as 5 pg/mL of 8-OHdG. This method promises to be very easily modified to microfluidic systems as "lab on valve". The optimized method had sufficient selectivity and thus could be used for determination of 8-OHDG in human urine and therefore for estimation of oxidative DNA damage as a result of oxidation stress in prostate cancer patients.

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“…Paramagnetic or superparamagnetic particles/beads (MPs) represent promising tool for this purpose [29][30][31]. MPs, whose size is ranging from nanometer to millimeter, respond to external magnetic field and facilitate bioactive molecules binding because of their affinity for the MPmodified surface made of biological components [32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Isolation of metallothionein from cells derived from aggressive form of high‐grade prostate carcinoma using paramagnetic antibody‐modified microbeads off‐line coupled with electrochemical and electrophoretic analysis

et al. 2011

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Prostate cancer with altered zinc(II) cell metabolism is the second most frequently diagnosed cancer in developed countries. The alterations of zinc(II) metabolism can influence metabolism of other metal ions and can also be associated with the expression and translation of metal-binding proteins including metallothioneins. The aim of this article was to optimize immunoseparation protocol based on paramagnetic beads conjugated with protein G for the isolation of metallothionein. Isolated metallothionein was determined by differential pulse voltammetry Brdicka reaction and SDS-PAGE. Optimal conditions: antigen-binding time - 60 min, temperature - 70°C, and buffer composition and pH - acetate buffer, pH 4.3, were determined. Under the optimized conditions, lysates from 22Rv1 prostate cancer cells treated with various concentrations of cadmium(II) and copper(II) ions were analyzed. We observed strong correlation in all experimental groups and all lysate types (r>0.83 at p<0.041) between metallothionein concentration related to viability and concentration of copper(II) ions and cadmium(II) ions in medium. Moreover, the results were compared with standard sample preparation as heat treatment and SDS-PAGE analysis.

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Magnetic bead-based chemiluminescence detection of sequence-specific DNA by using catalytic nucleic acid labels

Cited by 37 publications

References 32 publications

Improved method for chemiluminescent determination of peroxidase-mimicking DNAzyme activity

Improved method for chemiluminescent determination of peroxidase-mimicking DNAzyme activity

Microfluidic tool based on the antibody‐modified paramagnetic particles for detection of 8‐hydroxy‐2′‐deoxyguanosine in urine of prostate cancer patients

Isolation of metallothionein from cells derived from aggressive form of high‐grade prostate carcinoma using paramagnetic antibody‐modified microbeads off‐line coupled with electrochemical and electrophoretic analysis

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