Magnetic biosensor for the detection of Yersinia pestis

Meyer, M Cobas; Stehr, Matthias; Bhuju, Sabin; Krause, Hans‐Joachim; Hartmann, Markus; Miethe, Peter; Singh, Mahavir; Keusgen, Michael

doi:10.1016/j.mimet.2006.08.004

Cited by 78 publications

(46 citation statements)

References 20 publications

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“…Magnetic immunodetection has a significantly greater dynamic range than the ELISA, leading to saturation at higher analyte concentrations. Magnetic immunodetection thus achieves virus quantification over a wider concentration range than the ELISA, which is consistent with the results of magnetic immunoassays for the detection of human pathogens (20)(21)(22).…”

Section: Discussionsupporting

confidence: 82%

“…The magnetic immunodetection of GFLV was assessed using GFLV-infected plant material with capsid protein concentrations of 12 to 60 ng/ml for calibration. mAbFL 6 was immobilized on the polyethylene matrix of the immunofiltration column, and the biotinylated form was also linked to streptavidin-coated magnetic beads (21,22). The magnetic immunodetection method was compared with a standard double-antibody sandwich ELISA over the same concentration range (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…GFLVspecific antibodies were combined with magnetic nanoparticles to detect the virus and measure the infection grade. A similar method based on magnetic nanoparticles linked to specific antibodies has been developed for the detection and quantification of human pathogens (20)(21)(22)). An immunoassay approach was developed for the detection of bacterial and viral plant pathogens and achieves multiplex detection using different sets of fluorescence-coded microspheres (23).…”

Section: Potato Virus X (Pvx) and Tobacco Mosaic Virus (Tmv)mentioning

confidence: 99%

“…The functionalization of the outer shell allows the magnetic nanoparticles to be linked to specific antibodies, enabling the magnetic labeling of the corresponding antigens, as demonstrated with human pathogens (21,22). Here we used this technique for the first time to detect plant pathogens and achieved the rapid and quantitative detection of GFLV for phytosanitary applications.…”

Section: Potato Virus X (Pvx) and Tobacco Mosaic Virus (Tmv)mentioning

confidence: 99%

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Simple and Portable Magnetic Immunoassay for Rapid Detection and Sensitive Quantification of Plant Viruses

Rettcher

Jungk

Kühn

et al. 2015

Appl Environ Microbiol

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c Plant pathogens cause major economic losses in the agricultural industry because late detection delays the implementation of measures that can prevent their dissemination. Sensitive and robust procedures for the rapid detection of plant pathogens are therefore required to reduce yield losses and the use of expensive, environmentally damaging chemicals. Here we describe a simple and portable system for the rapid detection of viral pathogens in infected plants based on immunofiltration, subsequent magnetic detection, and the quantification of magnetically labeled virus particles. Grapevine fanleaf virus (GFLV) was chosen as a model pathogen. Monoclonal antibodies recognizing the GFLV capsid protein were immobilized onto immunofiltration columns, and the same antibodies were linked to magnetic nanoparticles. GFLV was quantified by immunofiltration with magnetic labeling in a double-antibody sandwich configuration. A magnetic frequency mixing technique, in which a two-frequency magnetic excitation field was used to induce a sum frequency signal in the resonant detection coil, corresponding to the virus concentration within the immunofiltration column, was used for high-sensitivity quantification. We were able to measure GFLV concentrations in the range of 6 ng/ml to 20 g/ml in less than 30 min. The magnetic immunoassay could also be adapted to detect other plant viruses, including Potato virus X and Tobacco mosaic virus, with detection limits of 2 to 60 ng/ml.

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Section: Discussionsupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Section: Potato Virus X (Pvx) and Tobacco Mosaic Virus (Tmv)mentioning

confidence: 99%

Section: Potato Virus X (Pvx) and Tobacco Mosaic Virus (Tmv)mentioning

confidence: 99%

See 2 more Smart Citations

Simple and Portable Magnetic Immunoassay for Rapid Detection and Sensitive Quantification of Plant Viruses

Rettcher

Jungk

Kühn

et al. 2015

Appl Environ Microbiol

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“…Meyer 43 , et al presented a magnetic biosensor for Yersinia pestis, which is the causative agent of the plague. It is a non-motile, gram-negative bacterium.…”

Section: Magnetic Nanoparticle-based Biosensorsmentioning

confidence: 99%

Nanoparticle-based Sensors

Khanna

2008

DSJ

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Nanoparticles exhibit several unique properties that can be applied to develop chemical and biosensors possessing desirable features like enhanced sensitivity and lower detection limits. Gold nanoparticles are coated with sugars tailored to recognise different biological substances. When mixed with a weak solution of the sugar-coated nanoparticles, the target substance, e.g., ricin or E.coli, attaches to the sugar, thereby altering its properties and changing the colour. Spores of bacterium labeled with carbon dots have been found to glow upon illumination when viewed with a confocal microscope. Enzyme/nanoparticle-based optical sensors for the detection of organophosphate (OP) compounds employ nanoparticle-modified fluorescence of an inhibitor of the enzyme to generate the signal for the OP compound detection. Nanoparticles shaped as nanoprisms, built of silver atoms, appear red on exposure to light. These nanoparticles are used as diagnostic labels that glow when target DNA, e.g., those of anthrax or HIV, are present. Of great importance are tools like gold nanoparticle-enhanced surface-plasmon resonance sensor and silver nanoparticle surface-enhanced portable Raman integrated tunable sensor. Nanoparticle metal oxide chemiresistors using micro electro mechanical system hotplate are very promising devices for toxic gas sensing. Chemiresistors comprising thin films of nanogold particles, encapsulated in monomolecular layers of functionalised alkanethiols, deposited on interdigitated microelectrodes, show resistance changes through reversible absorption of vapours of harmful gases. This paper reviews the state-of-the-art sensors for chemical and biological terror agents, indicates their capabilities and applications, and presents the future scope of these devices.

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