Magnetic Cell Manipulation and Sorting

Zborowski, Maciej; Chalmers, Jeffrey J.; Lowrie, William G.

doi:10.1007/978-3-319-44139-9_2

Cited by 10 publications

(6 citation statements)

References 77 publications

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“…Such an observation demonstrates that the current method has a great advantage for cell manipulation over other magnetophoresis techniques. As we briefly reviewed in Section , so far most cell manipulation techniques based on the magnetophoresis principle have to first make the cell magnetic either by coating the cells with magnetic particles or ingesting magnetic nanoparticle into the cells . The addition of particles on cell surfaces or inside cells inherently changes the cell performance, and care needs to be paid to interpret the activity of the cells after manipulation or extra works has to be done in order to remove the magnetic particles from cells.…”

Section: Resultsmentioning

confidence: 99%

“…A majority of these manipulation methods are based on magnetophoresis, in which magnetic particles move toward position with stronger field strength in nonuniform B‐fields. Nonmagnetic particles/cells can be manipulated magnetically by tethering magnetic particles to cells or by using a specially designed magnetic structure to drive them . For example, by labeling HeLa cells with magnetic nanoparticles, Ho et al prepared magnetic multicellular spheroids and demonstrated cell separation by applying a B‐field, without the need of centrifugation .…”

Section: Introductionmentioning

confidence: 99%

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Manipulation of Single Cells Using a Ferromagnetic Nanorod Cluster Actuated by Weak AC Magnetic Fields

et al. 2018

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A unique noncontact single cell manipulation technique based on the actuation of magnetic nanorods (MNRs) or clusters (MCs) by nonuniform alternating magnetic fields (nuAMFs) is demonstrated. Compared to the actuation of MNRs/MCs by conventional magnetophoresis, the motion of MNRs/MCs actuated by nuAMFs can be tuned by additional parameters including the shape of MNRs/MCs and the frequency of the applied magnetic fields. The manipulation of a single cell by an actuated MNR/MC are divided into five stages, i.e., approaching, pushing, carrying, dragging, and releasing. The interactions between the MNR/MC and the cell in these stages are investigated in detail both experimentally and numerically. Other applications of cell manipulation, such as concentrating cells at target locations and accumulating MNRs/MCs onto a single cell, are also demonstrated. The single cell manipulation system is simple, low‐cost, and low‐power consumption, and helps advance the state‐of‐the‐art of single‐particle manipulation.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Manipulation of Single Cells Using a Ferromagnetic Nanorod Cluster Actuated by Weak AC Magnetic Fields

et al. 2018

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“…The magnetically responsive carrier will move to the area with the highest density of the lines. Ordinarily, all biological and organic materials are diamagnetic with low magnetic susceptibility and negative magnetization response [98,99]. Therefore, the delivery carriers modified with magnetic particles possessing a positive magnetization response and high magnetic susceptibility can be selectively localized by the non-uniform magnetic field [100].…”

Section: Physical Addressing and Release Of Encapsulated Drugs By External Stimuli In Vivo Principles And Safety Considerations 41 Remotementioning

confidence: 99%

Key Points in Remote-Controlled Drug Delivery: From the Carrier Design to Clinical Trials

Voronin

Abalymov

Svenskaya

et al. 2021

IJMS

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The increased research activity aiming at improved delivery of pharmaceutical molecules indicates the expansion of the field. An efficient therapeutic delivery approach is based on the optimal choice of drug-carrying vehicle, successful targeting, and payload release enabling the site-specific accumulation of the therapeutic molecules. However, designing the formulation endowed with the targeting properties in vitro does not guarantee its selective delivery in vivo. The various biological barriers that the carrier encounters upon intravascular administration should be adequately addressed in its overall design to reduce the off-target effects and unwanted toxicity in vivo and thereby enhance the therapeutic efficacy of the payload. Here, we discuss the main parameters of remote-controlled drug delivery systems: (i) key principles of the carrier selection; (ii) the most significant physiological barriers and limitations associated with the drug delivery; (iii) major concepts for its targeting and cargo release stimulation by external stimuli in vivo. The clinical translation for drug delivery systems is also described along with the main challenges, key parameters, and examples of successfully translated drug delivery platforms. The essential steps on the way from drug delivery system design to clinical trials are summarized, arranged, and discussed.

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“…The application of the external magnetic field is another reliable way of highly selective cell manipulation by the external force [155]. This is achievable since biological materials have a very low magnetic susceptibility, and thus, the cells labeled with magnetic particles or that having the intrinsic magnetic properties can be effectively isolated without the interference with the surrounding medium and objects [156]. Therefore, the essential prerequisite of the magnetic sorting is a high magnetic response of the isolated objects (or its absence in case of the negative sorting).…”

Section: Labeling By Magnetic Beadsmentioning

confidence: 99%

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Voronin

Kozlova

Verkhovskii

et al. 2020

IJMS

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Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection of rare pathogenic objects in patient blood. These objects can be circulating tumor cells, very rare during the early stages of cancer development, various microorganisms and parasites in the blood during acute blood infections. All of these rare diagnostic objects can be detected and identified very rapidly to save a patient’s life. This review outlines the main techniques of visualization of rare objects in the blood flow, methods for extraction of such objects from the blood flow for further investigations and new approaches to identify the objects automatically with the modern deep learning methods.

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Magnetic Cell Manipulation and Sorting

Cited by 10 publications

References 77 publications

Manipulation of Single Cells Using a Ferromagnetic Nanorod Cluster Actuated by Weak AC Magnetic Fields

Manipulation of Single Cells Using a Ferromagnetic Nanorod Cluster Actuated by Weak AC Magnetic Fields

Key Points in Remote-Controlled Drug Delivery: From the Carrier Design to Clinical Trials

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

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