1985
DOI: 10.1016/s0021-9258(17)39618-7
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Magnetic circular dichroism studies of succinate dehydrogenase. Evidence for [2Fe-2S], [3Fe-xS], and [4Fe-4S] centers in reconstitutively active enzyme.

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Cited by 76 publications
(9 citation statements)
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“…MCD Spectroscopy. Low-temperature MCD measurements were carried out on the instrumentation previously described (Johnson et al, 1985). MCD spectra are corrected for natural CD and expressed at St ( < = eL -eR), which is the difference between the molar extinction coefficients of left and right circularly polarized light.…”
Section: Methodsmentioning
confidence: 99%
“…MCD Spectroscopy. Low-temperature MCD measurements were carried out on the instrumentation previously described (Johnson et al, 1985). MCD spectra are corrected for natural CD and expressed at St ( < = eL -eR), which is the difference between the molar extinction coefficients of left and right circularly polarized light.…”
Section: Methodsmentioning
confidence: 99%
“…The spin relaxation of cluster S-l decreases as cluster S-3 becomes reduced. Cluster S-3 is a trinuclear cluster which has a paramagnetic state when it is both oxidized and reduced (Johnson et al, 1985;Ackrell et al, 1984). Cluster S-3 in the oxidized state has a Kramer's S = 1/2 spin system, which is similar to many other reduced ferredoxins.…”
Section: Discussionmentioning
confidence: 99%
“…The structural genes for the Fp and the Ip subunit of Escherichia coli (sdhA and sdhB) (Wood et al, 1984; Darlinson & Guest, 1984) and B. subtilis (sdhB and sdhC) (Philips, Magnusson, Rutberg and Guest, unpublished observations) have been sequenced. The predicted amino acid sequences of Studies, principally with mitochondrial succinate dehydrogenase, have shown that there are three iron-sulfur clusters, designated as S-l, S-2, and S-3 in the enzyme (Ohnishi & Salerno, 1982; Beinert & Albracht, 1982; Johnson et al, 1985). Clusters S-l and S-3 can be reduced with succinate, but cluster S-2 can only be reduced with a strong reductant such as dithionite.…”
mentioning
confidence: 99%
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