Magnetic enzyme membranes as active elements of electrochemical sensors. Specific amino acid enzyme electrodes

Magnetic enzyme membranes as active elements of electrochemical sensors. Lactose, saccharose, maltose bienzyme electrodes

“…The enzyme electrode is based on the transformation of kinetic phenomena (reaction rates) into absolute values &:a1 concentrations) through the diffusion-reaction coupling process. The manufacture of magnetic enzyme electrodes [6] allows convenient use of the active sensors. The pOZ electrode has some advantages, namely the specificity based on the selectivity of the gas permeable membrane and the linear relationship between the oxygen and the output of the electrode.…”

“…The procedure for the manufacture of magnetic enzyme membranes was described in the preceeding paper [6]. 10 mg of /3-galactosidase (Kyowa Hakko) or 16 mg of invertase (Rapidase, from Saccharomyces cerevisiae) or 10 mg of maltase (Sigma, from Aspergillus niger) with 40 1.U.…”

“…The magnetic enzyme membrane is maintained by the magnetic field on a pOZ electrode (E 5040 Radiometer) modified according to the design described in the preceeding paper [6]. Magnetic materials were purchased from La Radiotechnique.…”

“…Hervagault et al [4] and Lecoq et al [5] have published theoretical and experimental studies on protein.membranes bearing bienzyme systems. Enzyme electrodes were introduced in the preceeding paper [6] but the present one is the first dealing with bienzyme membranes as active elements of an electrochemical sensor. The results presented were obtained with a sensor based on a pOz electrode.…”

Improved nonporous magnetic supports for immobilized enzymes

“…The initial studies on magnetic supports for immobilized enzymes (IMEs), in this laboratory and elsewhere, were summarized by Munro et al ' Recently, Calvot et al 2 have prepared magnetic enzyme membranes, while Horisberger3 and Chaplin and Kennedy4 have studied the immobilization of enzymes to magnetite and Ni powders, respectively.…”