Magnetic field inhibits isolated lymphocytes' proliferative response to mitogen stimulation

Abstract

We aimed to find out how the exposure of isolated lymphocytes to a pulsed magnetic field (MF) affected their in vitro proliferative response to mitogenic stimulation. Cells were exposed to MF of various intensities (0.3, 0.6, and 1.2 T) at a constant frequency of 30 Hz, for a period of 60, 180, and 330 s. Then, the proliferative response of splenocytes was induced by optimal concentrations of concanavalin A (Con A; mitogenic toward T cells), bacterial lipopolysaccharide (LPS; mitogenic toward B cells), or pokeweed mitogen (PWM; mitogenic toward both populations). We found that the exposure of lymphocytes to the MF profoundly inhibited their proliferative response to mitogens. The suppressive action of the MF on B and T cell proliferation was intensified when a cooperative response of those two lymphocyte populations was simultaneously induced by PWM. The inhibitory effect of MF depended on the exposure time and MF intensity. Prolonged exposure and/or a stronger intensity of the MF weakened its inhibitory influence on the response of lymphocyte to mitogenic stimulation. The data show that an exposure to MF may influence the activity of lymphocytes in their response to mitogenic stimuli.

A static magnetic field attenuates lipopolysaccharide-induced neuro-inflammatory response via IL-6-mediated pathway

“…Although their report indicated that the central nervous system organs exhibited endotoxin tolerance, clinical application of endotoxin tolerance for controlling neuro-inflammation remains unavailable, because it is very difficult to control the location and concentration of low-level LPS injection accurately in the brain. To overcome this problem and to halt degenerative disorders such as Alzheimer's, Parkinson's and Huntington's diseases, transcranial magnetic stimulation (TMS) with nonionizing radiation (frequencies from 0 to 300 GHz) was recently proposed to control the progression of neurodegeneration (Brehmer et al, 2012;Medina & Túnez, 2010;Roman et al, 2002Roman et al, , 2005. It was reported that TMS reduced oxidative damage to brain tissue induced by reactive oxygen species (ROS) resulting in a reduction of disease symptoms (Medina & Túnez, 2010;Tasset et al, 2012Tasset et al, , 2013Túnez et al, 2006).…”

Common behaviors alterations after extremely low-frequency electromagnetic field exposure in rat animal model

“…Several research were carried out to study the influence of whole body exposure to ELF-EMF on human and animal health. Roman et al (2005) reported the reduction in cell proliferation under exposure to ELF. Researchers also identify an increase in the concentration of Na + , K + and osmolarity after irradiated with ELF, and suggest that there is a relationship between these factors and inhibition of cell growth.…”

Reversible Effect of Magnetic Fields on Human Lymphocyte Activation Patterns: Different Sensitivity of Naive and Memory Lymphocyte Subsets

“…In our study, cell proliferation was evaluated using an anti-Ki-67 monoclonal antibody (24). The Ki-67 molecule is the most widely used marker for proliferation in clinical practice; it is expressed in all active phases of the cell cycle and is absent in quiescent cells.…”