We present high-resolution (0. ′′ 4) mid-infrared (mid-IR) polarimetric images and spectra of WL 16, a Herbig Ae star at a distance of 125 pc. WL 16 is surrounded by a protoplanetary disk of ∼ 900 AU in diameter, making it one of the most extended Herbig Ae/Be disks as seen in the mid-IR. The star is behind, or embedded in, the ρ Ophiuchus molecular cloud, and obscured by 28 magnitudes of extinction at optical wavelengths by the foreground cloud. Mid-IR polarization of WL 16, mainly arises from aligned elongated dust grains present along the line of sight, suggesting a uniform morphology of polarization vectors with an orientation of 33 • (East from North) and a polarization fraction of ∼ 2.0%. This orientation is consistent with previous polarimetric surveys in the optical and near-IR bands to probe large-scale magnetic fields in the Ophiuchus star formation region, indicating that the observed mid-IR polarization toward WL 16 is produced by the dichroic absorption of magnetically aligned foreground dust grains by a uniform magnetic field. Using polarizations of WL 16 and Elias 29, a nearby polarization standard star, we constrain the polarization efficiency, p 10.3 /A 10.3 , for the dust grains in the ρ Ophiuchus molecular cloud to be ≃ 1.0% mag −1 . WL 16 has polycyclic aromatic hydrocarbon (PAH) emission features detected at 8.6, 11.2, 12.0, and 12.7 µm by our spectroscopic data, and we find an anti-correlation between the PAH surface brightness and the PAH ionization fraction between the NW and SW sides of the disk.