In this study, we aimed at optimizing the parameters which govern the separation efficiency in the magnetic filtration of magnetizable dispersed particles from a water medium. Response surface methodology (RSM) was used to determine the influence of the process parameters. Also, the optimal processing conditions were determined in order to reduce the external magnetic field strength, diameter of the filter matrix elements, and filter length to a safe level. A three-level central composite design (CCRD) involving the variables, such as external magnetic field strength (148-282 kA/m), diameter of the filter elements (0.005-0.011 m), and the filter length (0.01-0.10 m) was developed for this purpose. Data obtained from the RSM was subjected to the analysis of variance (ANOVA) using a second-order polynomial equation, which provided the optimized process conditions as 298 kA/m for external magnetic field strength, 0.0015 m for diameter of the filter elements, and 0.095 m for the filter length. The separation efficiency was optimized for magnetic filtration of micron sized particles that can be magnetized in an external magnetic field and the value was found to be 97%.