Magnetic-graphene oxide based molecular imprinted polymers for selective extraction of glycoprotein at physiological pH

Recent Advances in Quartz Crystal Microbalance Biosensors Based on the Molecular Imprinting Technique for Disease-Related Biomarkers

“…The non-covalent imprinting technique overcomes the limitations of the covalent imprinting method, which requires a difficult desorption process with a hard eluent through non-covalent interactions. Moreover, the surface imprinting technique provides a fast adsorption/elution rate, good stability, and an easy separation characteristic [121][122][123][124].…”

A selectivity‐enhanced colloidal crystal array for ribavirin sensing based on the synergistic effect of covalent and non‐covalent interactions

“…Molecular imprinting is a well‐known technique for the preparation of tailor‐made recognition materials by copolymerizing functional monomers in the presence of template molecules. Molecularly imprinted polymers (MIPs) have been widely used in the fields of solid phase extraction, biosensors, catalysis and drug delivery, owing to their distinguished advantages of specific recognition ability, good stability and a simple preparation process 29–33 . In view of their satisfactory specificity and high physicochemical stability, MIPs have been widely used in biosensors 29,34,35 .…”

“…Molecularly imprinted polymers (MIPs) have been widely used in the fields of solid phase extraction, biosensors, catalysis and drug delivery, owing to their distinguished advantages of specific recognition ability, good stability and a simple preparation process. [29][30][31][32][33] In view of their satisfactory specificity and high physicochemical stability, MIPs have been widely used in biosensors. 29,34,35 To this end, if the molecular imprinting and the CCAs are combined, a novel colorimetric sensor with excellent sensitivity and selectivity can be constructed.…”

Boronate Avidity Assisted by Dendrimer-like Polyhedral Oligomeric Silsesquioxanes for a Microfluidic Platform for Selective Enrichment of Ubiquitination and Glycosylation

“…Boronate affinity materials have become a powerful tool for the selective separation and enrichment of low-abundance glycoproteins and glycopeptides , since they rely on the well-known specific interaction of the boronic acid moiety with 1,2 or 1,3- cis -diol-containing compounds . By the selective capture of the cis -diol containing compounds under basic conditions and acidic elution, they can purify glycoproteins or glycopeptides with a mechanism like immunoaffinity. , Recently, boric acid-functionalized titania nanomaterials detected 26 glycopeptides successfully from horseradish peroxidase (HRP) digest with the detection limit of glycopeptides as low as 5 fmol . The binding strength and minimum extraction concentration of boric acid-functionalized magnetic nanoparticles to glycoproteins have reached 10 –5 –10 –10 M and 2 × 10 –14 M, respectively. , However, the complex between boronic acid and cis -diol groups are characterized by an affinity from 3 to 6 orders of magnitude in a dissociation constant (10 –1 < K d < 10 –4 M) lower than immunoaffinity interactions ( K d < 10 –7 M), , resulting in an undesired affinity and limited adsorption capacity of traditional boron affinity materials for application to real samples.…”

“…9 By the selective capture of the cis-diol containing compounds under basic conditions and acidic elution, they can purify glycoproteins or glycopeptides with a mechanism like immunoaffinity. 10,11 Recently, boric acidfunctionalized titania nanomaterials detected 26 glycopeptides successfully from horseradish peroxidase (HRP) digest with the detection limit of glycopeptides as low as 5 fmol. 12 The binding strength and minimum extraction concentration of boric acidfunctionalized magnetic nanoparticles to glycoproteins have reached 10 −5 −10 −10 M and 2 × 10 −14 M, respectively.…”