Magnetic levitating polymeric nano/microparticular substrates for three-dimensional tumor cell culture

Lee, Woong Ryeol; Oh, Kyung Taek; Park, So Young; Yoo, Na Young; Ahn, Yong Sik; Lee, Don Haeng; Youn, Yu Seok; Lee, Deok-Keun; Cha, Kyung-Hoi; Lee, Eun Seong

doi:10.1016/j.colsurfb.2011.02.021

Cited by 21 publications

(14 citation statements)

References 27 publications

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“…Several fields including 3-D cell culturing have adapted the magnetically driven platforms [98–103]. An ability to create 3-D tumor spheroids that mimic in vivo tumors by utilizing a magnetic cell levitation method has been demonstrated [104]. Magnetic iron oxide (Fe 3 O 4 )-encapsulated poly(lactic-co-glycolide)(PLGA) micro-particles or poly(L-lactic acid)- b -poly (ethylene glycol)-folate [PLLA- b -PEG-folate] nanoparticles were utilized as substrates for 3-D tumor cell culture.…”

Section: Technologies To Develop 3-d Cultures and Spheroidsmentioning

confidence: 99%

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Engineering cancer microenvironments for in vitro 3-D tumor models

et al. 2015

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The natural microenvironment of tumors is composed of extracellular matrix (ECM), blood vasculature, and supporting stromal cells. The physical characteristics of ECM as well as the cellular components play a vital role in controlling cancer cell proliferation, apoptosis, metabolism, and differentiation. To mimic the tumor microenvironment outside the human body for drug testing, two-dimensional (2-D) and murine tumor models are routinely used. Although these conventional approaches are employed in preclinical studies, they still present challenges. For example, murine tumor models are expensive and difficult to adopt for routine drug screening. On the other hand, 2-D in vitro models are simple to perform, but they do not recapitulate natural tumor microenvironment, because they do not capture important three-dimensional (3-D) cell–cell, cell–matrix signaling pathways, and multi-cellular heterogeneous components of the tumor microenvironment such as stromal and immune cells. The three-dimensional (3-D) in vitro tumor models aim to closely mimic cancer microenvironments and have emerged as an alternative to routinely used methods for drug screening. Herein, we review recent advances in 3-D tumor model generation and highlight directions for future applications in drug testing.

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Section: Technologies To Develop 3-d Cultures and Spheroidsmentioning

confidence: 99%

“…(iv) Optical image of KB tumor cell clusters (indicated by a white arrow) attached on the surface of magnetic PLGA microparticles. Reprinted by copyright permissions from [104]. (b) Human glioblastoma cells (lower arrow) mixed with magnetic Fe 3 O 4 -loaded containing hydrogel held at the air–medium interface by a magnet.…”

Section: Figurementioning

confidence: 99%

Engineering cancer microenvironments for in vitro 3-D tumor models

et al. 2015

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“…Using a large-radius magnet, it was observed that the shape of the cell pattern generated was ring-shaped and this pattern was maintained after the magnet was removed. The concept of magnetic levitation was also used in development of 3D tumor spheroids that mimic in vivo tumors for potential anticancer drug screening [119]. …”

Section: Tissue Engineering and Regenerative Medicinementioning

confidence: 99%

Magnetic Nanoparticle-Based Approaches to Locally Target Therapy and Enhance Tissue Regeneration In Vivo

et al. 2012

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Magnetic-based systems utilizing superparamagnetic nanoparticles and a magnetic field gradient to exert a force on these particles have been used in a wide range of biomedical applications. This review is focused on drug targeting applications that require penetration of a cellular barrier as well as strategies to improve the efficacy of targeting in these biomedical applications. Another focus of this review is regenerative applications utilizing tissue engineered scaffolds prepared with the aid of magnetic particles, the use of remote actuation for release of bioactive molecules and magneto–mechanical cell stimulation, cell seeding and cell patterning.

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“…For nanocarriers, which include liposomes, polymeric nanoparticles, and various types of nucleic acid vectors, the drug carrier typically is conjugated to folate, usually through a PEG linker to overcome steric hindrance surrounding the FR on the cell surface (64)(65)(66)(67)(68). Among macromolecules, folate has been conjugated to protein toxins (69), antibodies (70)(71)(72)(73)(74)(75), polymeric drug carriers (76)(77)(78)(79)(80), and prodrug converting enzyme (81). Clinical translation activities, however, have so far been focused on low molecular weight (MW) folate conjugates and have been carried out at Endocyte Inc.…”

Section: Folate Conjugates As Fr-targeted Therapeuticsmentioning

confidence: 99%

Folate receptor targeted drug delivery- from the bench to the bedside

Xie¹,

Zhou²,

Zhang³

et al. 2016

Eur J Bio Med Res

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Abstract:Targeted drug delivery is a promising strategy for improving tumor therapy. Folate receptor (FR) is an established ovarian cancer marker that is also frequently found to be overexpressed in other major epithelial tumors. Meanwhile, functional FR−β is expressed in myeloid leukemias and in inflammatory macrophages, including tumor-associated macrophages. FR based tumor targeted drug delivery has been a very active area of research and recent advancement of this technology towards clinical translation is highly significant.

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Magnetic levitating polymeric nano/microparticular substrates for three-dimensional tumor cell culture

Cited by 21 publications

References 27 publications

Engineering cancer microenvironments for in vitro 3-D tumor models

Engineering cancer microenvironments for in vitro 3-D tumor models

Magnetic Nanoparticle-Based Approaches to Locally Target Therapy and Enhance Tissue Regeneration In Vivo

Folate receptor targeted drug delivery- from the bench to the bedside

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