Magnetic mesoporous silica nanoparticles: Fabrication and their laccase immobilization performance

Wang, Rui; Chen, Guo; Yang, Liangrong; Liu, Chun-Zhao

doi:10.1016/j.biortech.2010.06.115

Cited by 183 publications

(86 citation statements)

References 35 publications

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“…This may have been caused by restricted mobility of the bound enzyme and reduced accessibility of the substrate to the active site. Similar higher K m for immobilized enzyme has been reported for silica immobilized laccase [34]. The K m for b-glucosidase immobilized on Eupergit C was ten times higher than that of the free enzyme [35].…”

Section: Kinetic Characterization Of the Immobilized Proteasesupporting

confidence: 79%

Immobilization of halophilic Bacillus sp. EMB9 protease on functionalized silica nanoparticles and application in whey protein hydrolysis

Sinha

Khare

2014

Bioprocess Biosyst Eng

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The present work targets the fabrication of an active, stable, reusable enzyme preparation using functionalized silica nanoparticles as an effective enzyme support for crude halophilic Bacillus sp. EMB9 protease. The immobilization efficiency under optimized conditions was 60%. Characterization of the immobilized preparation revealed marked increase in pH and thermal stability. It retained 80% of its original activity at 70 °C while t 1/2 at 50 °C showed a five-fold enhancement over that for the free protease. Kinetic constants K m and V max were indicative of a higher reaction velocity along with decreased affinity for substrate. The preparation could be efficiently reused up to 6 times and successfully hydrolysed whey proteins with high degree of hydrolysis. Immobilization of a crude halophilic protease on a nanobased scaffold makes the process cost effective and simple.

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Section: Kinetic Characterization Of the Immobilized Proteasesupporting

confidence: 79%

Immobilization of halophilic Bacillus sp. EMB9 protease on functionalized silica nanoparticles and application in whey protein hydrolysis

Sinha

Khare

2014

Bioprocess Biosyst Eng

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“…Silica nanoparticles are attractive candidates as inorganic, nanoscale (less than or equal to 100 nm in diameter) materials that have received considerable attention in recent years, particularly in medicine, due to their inert, biocompatible, biodegradable, thermally and chemically stable properties [1][2][3][4] as well as their inexpensive synthesis [5]. In particular, mesoporous silica nanoparticles (MSNPs) have received interest as carriers for many applications, including pharmaceutical drug delivery, gene therapy, enzyme immobilization, catalytic chemistry, ion exchange, biosensing and bioimaging [6][7][8][9][10][11][12]. Furthermore, the ordered pore network, high pore volume and surface area, and silanol-containing surface [13] as well as the biodegradation of silica in the natural environment into relatively harmless silicic acid by-products make this an attractive proposition.…”

Section: Introductionmentioning

confidence: 99%

Effective delivery of volatile biocides employing mesoporous silicates for treating biofilms

Chan

Cadena

Townley

et al. 2017

J. R. Soc. Interface.

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Nanoparticulate delivery of biocides has the potential to decrease levels of exposure to non-target organisms, and miminize long-term exposure that can promote the development of resistance. Silica nanoparticles are an ideal vehicle since they are inert, biocompatible, biodegradable, and thermally and chemically stable. Encapsulation of biocides within nanoparticulates can improve their stability and longevity and maximize the biocidal potential of hydrophobic volatile compounds. Herein, we have shown that the plant secondary metabolites allyl isothiocyanate and cinnamaldehyde demonstrated increased antimicrobial activity against Escherichia coli in planktonic form, when packaged into mesoporous silica nanoparticles. Furthermore, the biocide-loaded nanoparticles showed activity against Pseudomonas aeruginosa biofilms that have inherent resistance to antimicrobial agents. The delivery platform can also be expanded to traditional biocides and other non-conventional antimicrobial agents.

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“…The solution to this problem is to use immobilized enzymes that are more stable and therefore reusable. Enzyme immobilization, especially for decolorization of azo dyes, has been introduced using some polymeric carriers including polyamide 6,6 fibers , alginate/ gelatin blend with PEG (Ping et al 2008), porous poly (GMA/EGDMA) (Arica et al 2009), and silica (Wang et al 2010). However, some of these polymers have low mechanical strength, durability, and/or high cost.…”

Section: Introductionmentioning

confidence: 99%

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

Bankeeree¹,

Prasongsuk²,

Imai³

et al. 2016

BioResources

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In an attempt to find a more efficient technique for biodegradation of the recalcitrant Reactive Black 5 (RB-5) dye, a composite xylan-polyvinyl alcohol (xylan-PVOH) hydrogel was used to immobilize laccase from the white-rot fungus Trametes versicolor. Xylan was prepared from the black liquor of pulp and paper effluent, and it was esterified with citric acid prior to cross-linking with polyvinyl alcohol (PVOH). The optimum composition for the immobilized laccase bead formation consisted of 4% (w/v) modified xylan, 10% (w/v) PVOH, and 15 U.mL -1 crude laccase. The maximum decolorization of RB-5 (98.45  1.96 %) was obtained within the first cycle (6 h) at 40 °C. In the eighth cycle, the reused beads were able to decolorize 55.35  2.46 % of the RB-5. Moreover, the xylan-PVOH beads extended the optimum pH range of laccase activity from 6 to 10 and tolerated a temperature up to 10 °C higher than that of the free enzyme. These results suggest that the xylan-PVOH bead has great potential as the polymer matrix for enzyme immobilization, which has applications in wastewater treatment.

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Magnetic mesoporous silica nanoparticles: Fabrication and their laccase immobilization performance

Cited by 183 publications

References 35 publications

Immobilization of halophilic Bacillus sp. EMB9 protease on functionalized silica nanoparticles and application in whey protein hydrolysis

Immobilization of halophilic Bacillus sp. EMB9 protease on functionalized silica nanoparticles and application in whey protein hydrolysis

Effective delivery of volatile biocides employing mesoporous silicates for treating biofilms

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

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