Magnetic metal-organic frameworks immobilized enzyme-based nano-biocatalytic systems for sustainable biotechnology

Bilal, Muhammad; Rashid, Ehsan Ullah; Munawar, Junaid; Iqbal, Hafiz M.N.; Cui, Jiandong; Zdarta, Jakub; Ashraf, S. Salman; Jesionowski, Teofil

doi:10.1016/j.ijbiomac.2023.123968

Cited by 28 publications

(9 citation statements)

References 167 publications

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“…Among the supports, magnetite (Fe 3 O 4 ) stands out as the most widely used MNP for enzyme immobilization 189 because of its cost-effectiveness, biocompatibility, low toxicity, large surface area because of small particle size, high magnetic susceptibility, high saturation magnetization, and superparamagnetic properties at room temperature. 189,194,199,200 Consequently, immobilization of enzymes on MNPs signicantly improves stability, catalytic performance, and reusability compared to pure enzymes. 189,200,201 2.2.…”

Section: Magnetic Propertiesmentioning

confidence: 99%

“…189,194,199,200 Consequently, immobilization of enzymes on MNPs signicantly improves stability, catalytic performance, and reusability compared to pure enzymes. 189,200,201 2.2. Superparamagnetism Among the properties of nanomaterials, superparamagnetism occurs when the magnetic material is reduced in size, such as between 10 nm and 150 nm in diameter 202,203 and presents a single-state domain in which the magnetic spins are aligned 202 (Fig.…”

Section: Magnetic Propertiesmentioning

confidence: 99%

“…189,194,199,200 Consequently, immobilization of enzymes on MNPs significantly improves stability, catalytic performance, and reusability compared to pure enzymes. 189,200,201…”

Section: Magnetic Nanomaterialsmentioning

confidence: 99%

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Advancements in enzyme immobilization on magnetic nanomaterials: toward sustainable industrial applications

Gama Cavalcante,

Dari,

Izaias da Silva Aires

et al. 2024

RSC Adv.

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Section: Magnetic Propertiesmentioning

confidence: 99%

Section: Magnetic Propertiesmentioning

confidence: 99%

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Advancements in enzyme immobilization on magnetic nanomaterials: toward sustainable industrial applications

Gama Cavalcante,

Dari,

Izaias da Silva Aires

et al. 2024

RSC Adv.

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“…Surface modification of nanomaterials is a key process that enhances their properties and functionality. , It involves the alteration of the surface chemistry and morphology of nanoparticles to improve their solubility, stability, and biocompatibility. , Therefore, the formation of nanomaterials is a valuable method in the field of nanoscale engineering, presenting numerous appealing concepts and opportunities in various scientific domains. Over the past few years, there has been an investigation into various types of meticulously arranged nanoscale configurations that can be linked to different classes of biomolecules, such as carbohydrates, lipids, proteins, and nucleic acids. − Some of the commonly used techniques for surface modification of nanomaterials include chemical functionalization, physical adsorption, and covalent binding. , …”

Section: Introductionmentioning

confidence: 99%

Surface Engineering of Superparamagnetic Graphene Oxide Nanosheet as a Chemically Tunable Platform for Facial Biofuel Production by Lipase

Landarani-Isfahani,

Arabi,

Rezaei

et al. 2024

ACS Appl. Bio Mater.

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In this research, we utilized an efficient approach to synthesize superparamagnetic graphene oxide (SPGO) rapidly in a one-pot method using microwave irradiation of graphene oxide (GO), urea, and Fe(III) ion. Tannic acid (TA) was introduced to the surface of SPGO through a straightforward and eco-friendly process. Methods were devised to furnish GO nanosheets and modify their surfaces with TA in an environmentally friendly manner. Two series of nanosheets, namely, SPGO/TA-COOH and SPGO/TA-IM, were engineered on the surface and used for immobilizing lipase enzyme. Through various analytical tools, the unique biocatalysts SPGO/TA-COOH/L and SPGO/TA-IM/L were confirmed. These biocatalysts exhibited enhanced stability at high temperatures and pH levels compared with free lipase. They also demonstrated prolonged storage stability and reusability over four months and seven cycles, respectively. Furthermore, the catalytic activity of immobilized lipase showed minimal impairment based on kinetic behavior analysis. The kinetic constants of SPGO/TA-IM/L were determined as V max = 0.24 mM min −1 , K m = 0.224 mM, and k cat = 0.8 s −1 . Additionally, the efficiency of biocatalysts for biodiesel production from palmitic acid was studied, focusing on various reaction parameters, such as temperature, alcohol to palmitic acid molar ratio, water content, and lipase quantity. The esterification reaction of palmitic acid with methanol, ethanol, and isopropanol was tested in the presence of SPGO/TA-COOH/L and SPGO/TA-IM/L, and the corresponding esters were obtained with a yield of 30.6−91.6%.

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“…It is necessary to focused primarily on recent trends in green enzyme evolution and immobilization biotechnology around the potential industrial applications of enzymes in various sectors of the modern industry.To solve these problems, enzyme immobilization approaches have been adopted as parallel or mutually auxiliary strategies for improving performance of enzyme. Recent reports show efforts on improving both enzymatic activity and stability through immobilization (Bilal et al, 2023). The major issue for obtaining improved biocatalysts using in industrial biotechnology is how to remold enzyme with mild, simple, and effective methods, especially in the actual complex catalytic environment.…”

mentioning

confidence: 99%

Editorial: Enzyme immobilization technologies and their biomanufacturing applications

Cui,

Ocsoy,

Mahmoud

et al. 2023

Front. Bioeng. Biotechnol.

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Editorial on the Research Topic Enzyme immobilization technologies and their biomanufacturing applicationsWith the looming apprehension of climate change, extensive environmental deterioration and mass extinctions, the transition to a greener, environmentally friendly, and sustainable production of liquid fuels and platform chemicals has become imperative. Biotechnological processing by enzymes has been used widely in a wide range of industrial sectors including chemicals, pharmaceuticals, food and feed, detergents, pulp and paper, textiles, energy, materials, and polymers. From the last several years ligninolytic enzymes find applications in numerous industrial processes (Sheldon et al., 2020;Wu et al., 2021). However, their lower catalytic efficiencies and operational stabilities limit their practical and multipurpose applications in various sectors of the current industrial processes (Ren et al., 2019;Feng et al., 2022). It is necessary to focused primarily on recent trends in green enzyme evolution and immobilization biotechnology around the potential industrial applications of enzymes in various sectors of the modern industry.To solve these problems, enzyme immobilization approaches have been adopted as parallel or mutually auxiliary strategies for improving performance of enzyme. Recent reports show efforts on improving both enzymatic activity and stability through immobilization (Bilal et al., 2023). The major issue for obtaining improved biocatalysts using in industrial biotechnology is how to remold enzyme with mild, simple, and effective methods, especially in the actual complex catalytic environment. With the rapid development of chemistry, computer, materials and other disciplines, more and more methods have been used to optimize the design of immobilized enzyme. Enzyme immobilization is growing rapidly and will become a powerful norm in bio-catalysis with much controlled features, such as selectivity, specificity, stability, resistivity, induce activity, reaction efficacy, muti-usability, improved mass transfer efficiency, high catalytic turnover, optimal yield, ease in recovery, and cost-effectiveness. In addition, enzyme immobilization strategies for complex enzyme processes such as multi-enzyme catalysis and non-aqueous enzyme catalysis should be proposed as soon as possible (Ren et al., 2019).

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Magnetic metal-organic frameworks immobilized enzyme-based nano-biocatalytic systems for sustainable biotechnology

Cited by 28 publications

References 167 publications

Advancements in enzyme immobilization on magnetic nanomaterials: toward sustainable industrial applications

Advancements in enzyme immobilization on magnetic nanomaterials: toward sustainable industrial applications

Surface Engineering of Superparamagnetic Graphene Oxide Nanosheet as a Chemically Tunable Platform for Facial Biofuel Production by Lipase

Editorial: Enzyme immobilization technologies and their biomanufacturing applications

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